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Journals / Turkish Journal of Gastroenterology / 2002 / Cilt: 13 - Sayı: 2

Gastrik biyopsi örneklerinde Helicobacter pylori'nin "nested" polimeraz zincir reaksiyonu ve hızlı ürea testi ile belirlenmesi

Detection of Helicobacter pylori using nested polymerase chain reaction and rapid urease test in gastric biopsy

Dönmez Hamiyet ALTUNTAŞ
Kadri GÜVEN
Pages
94–97
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Conclusions: Our results indicate that the nested polymerase chain reaction is more specific and sensitive than the rapid urease test for detecting Helicobacter pylori in gastric biopsy samples.

📌 3 Key Takeaways

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Objective & Hypothesis:Background/aims: The purpose of this study was to compare nested polymerase chain reaction and rapid urease test findings in the diagnosis of Helicobacter pylori infection.
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Key Findings & Data:Methods: Two gastric biopsy specimens were obtained from each of 64 patients and polymerase chain reaction and rapid urease test were performed.
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Conclusion & Impact:Conclusions: Our results indicate that the nested polymerase chain reaction is more specific and sensitive than the rapid urease test for detecting Helicobacter pylori in gastric biopsy samples.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Duyarlılık ve özgüllük · Polimeraz zincir reaksiyonu · Helicobacter pylori

Abstract

Background/aims: The purpose of this study was to compare nested polymerase chain reaction and rapid urease test findings in the diagnosis of Helicobacter pylori infection. Methods: Two gastric biopsy specimens were obtained from each of 64 patients and polymerase chain reaction and rapid urease test were performed. DNA extraction was followed by amplification with two primer pairs from the urease A gene of Helicobacter pylori genome. Results: Fourty two patients (65.6%) had a positive rapid urease test result while 22 (34.4%) had a negative result for Helicobacter pylori. Sixty of 64 patients (93.8 %) had a positive result with the nested polymerase chain reaction method. Four patients with negative nested polymerase chain reaction results also had negative rapid urease test results. All of the eighteen patients with rapid urease test-negative results were positive with nested polymerase chain reaction. The number of nested polymerase chain reaction-positive patients (93.8%) was significantly higher than rapid urease test-positive (65.6%) and first round polymerase chain reaction-positive (53.1%) patients (P< 0.001). Conclusions: Our results indicate that the nested polymerase chain reaction is more specific and sensitive than the rapid urease test for detecting Helicobacter pylori in gastric biopsy samples.

Özet

Amaç: Altmışdört gastrik biyopsi örneğinde nested polimeraz zincir reaksiyonu ve hızlı ureaz testi yöntemleri kullanılarak Helicobacter pylori varlığı değerlendirildi. Bu çalışmanın amacı, Helicobacter pylori enfeksiyonunun teşhisinde nested polimeraz zincir reaksiyonu ve hızlı üreaz test sonuçlarını karşılaştırmaktır. Yöntem: "Nested" polimeraz zincir reaksiyonu ve hızlı ureaz testi için ayrı ayrı olmak üzere, tüm hastalardan iki gastrik biyopsi örneği alınmıştır. DNA izolasyonunu takiben Helicobacter pylori genomunun ureaz A genine uygun iki primer çiftiyle amplifikasyon yapılmıştır. Bulgular: Hızlı ureaz testi sonucuna göre, Helicobacter pylori için 42 hasta pozitif (%65.6) ve 22 hasta da negatif (%34.4) bulunmuştur. 64 hastanın altmışı (%93.8), "nested" polimeraz zincir reaksiyonu metodu ile pozitif bulunmuştur. "Nested" polimeraz zincir reaksiyonu ile negatif olan dört hasta hızlı ureaz test ile de negatiftir. Hızlı ureaz testi ile negatif olan 18 hastanın hepsi, "nested" polimeraz zincir reaksiyonu ile pozitif bulunmuştur. "Nested" polimeraz zincir reaksiyonu -pozitif hastalar (%93.8), hızlı ureaz test-pozitif (%65.6) ve ilk tur polimeraz zincir reaksiyonu -pozitif (%53.1) hastalara göre önemli derecede yüksektir (P< 0.001). Sonuç: Sonuçlarımız, gastrik biyopsi örneklerinde Helicobacter pylori'nin belirlenmesi için hızlı ureaz teste göre "nested"polimeraz zincir reaksiyonu metodunun daha spesifik ve duyarlı olduğunu göstermektedir.

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Cite This Article

ALTUNTAŞ, D. H., GÜVEN, K. (2002). Gastrik biyopsi örneklerinde Helicobacter pylori'nin "nested" polimeraz zincir reaksiyonu ve hızlı ürea testi ile belirlenmesi. Turkish Journal of Gastroenterology, Cilt: 13 - Sayı: 2, 94–97. https://ajindex.com/articles/410439

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