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Journals / Erciyes Tıp Dergisi / 2012 / Cilt: 34 - Sayı: 3

Fitohemaglütinin ile uyarılmış insan T-lenfositlerindeki agnor motiflerinin T-lenfosit alt grupları ile karşılaştırmalı olarak incelenmesi

Comparative studies of the agnors motifs in phytohemagglutinin-stimulated human T-lymphocytes with T-lymphocyte subgroups

Hamiyet Dönmez ALTUNTAŞ
Zuhal HAMURCU
Halil DEMİRTAŞ
Nazmiye BİTGEN
Figen ÖZTÜRK
Pages
132–136
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found that the different types of NOR motifs formed with AgNOR staining are not related to the T- lymphocyte subgroup.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Using of argyrophilic nucleolar organiser region (AgNOR) patterns, instead of the fluorescent T-lymphocyte markers, in specification of sub-groups of T-lymphocyte was researched in the study.
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Key Findings & Data:Results: As we expected, the CD8 staining is not observed in a single style of the nucleolar organiser region (NOR) motif, but rather occurred in the forms of different kinds of nucleolus.
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Conclusion & Impact:It was found that the different types of NOR motifs formed with AgNOR staining are not related to the T- lymphocyte subgroup.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Using of argyrophilic nucleolar organiser region (AgNOR) patterns, instead of the fluorescent T-lymphocyte markers, in specification of sub-groups of T-lymphocyte was researched in the study. For this, it was aimed to indicate that AgNOR pattern of each T-lymphocyte sub-group remains stable in a stage of cell cycle stimulated by phytohaemagglutinin (PHA) and that these patterns are different from each other. Material and Methods: Peripheral blood samples of four healthy volunteers were cultured according to whole blood and/or isolated blood culture methods. The slides were stained by using AgNOR and immunohistochemical staining methods. Results: As we expected, the CD8 staining is not observed in a single style of the nucleolar organiser region (NOR) motif, but rather occurred in the forms of different kinds of nucleolus. It was found that the different types of NOR motifs formed with AgNOR staining are not related to the T- lymphocyte subgroup. Conclusion: It was concluded that the NOR motifs are not specific to sub-groups of T-lymphocytes. Further investigations are needed to support our results.

Özet

Amaç: Bu çalışmada, T-lenfosit alt tiplerinin tanımlanmasında flöresanlı, T-lenfosit belirleyicileri yerine, gümüşle boyanabilen nükleer organize edici bölge (AgNOR) motiflerinin kullanımı araştırıldı. Bunun için, fitohemagulütinin (PHA) ile uyarılan T-lenfositlerde, alt tiplerinden her birinde oluşan AgNOR motifinin, belirli bir hücre döngüsü aşamasında sabit kaldığının ve bu motiflerin alt tipler arasında farklı olduğunun gösterilmesi amaçlandı. Gereç ve Yöntemler: Dört sağlıklı gönüllünün periferal kan örnekleri, tam kan ve/veya izole kan kültürü tekniklerine göre kültüre edildi. Hazırlanan preparatlar, AgNOR ve immünohistokimyasal boyama yöntemleri ile boyandı. Bulgular: Beklendiği gibi tek bir nükleer organize edici bölge (NOR) motifinde CD8 boyamanın görülmediği, aksine birden farklı çeşitteki çekirdekçik şekillerinde de CD8le boyanmanın gerçekleştiği, dolayısıyla AgNOR boyama ile oluşan değişik tipteki NOR motifleri ile T-lenfosit alt gruplarının bağlantılı olmadığı bulundu. Sonuç: NOR motiflerinin T-lenfosit alt gruplarına spesifik olmadığı sonucuna varılmıştır. Sonuçlarımızın desteklenmesi için konuyla ilgili başka çalışmaların yapılması gerekmektedir.

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Cite This Article

ALTUNTAŞ, H. D., HAMURCU, Z., DEMİRTAŞ, H., BİTGEN, N., ÖZTÜRK, F. (2022). Fitohemaglütinin ile uyarılmış insan T-lenfositlerindeki agnor motiflerinin T-lenfosit alt grupları ile karşılaştırmalı olarak incelenmesi. Erciyes Tıp Dergisi, Cilt: 34 - Sayı: 3, 132–136. https://ajindex.com/articles/502461

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References (17)

  1. Comings DE. Arrangement of chromatin in the nucleus. Hum Genet 1980; 53(2): 131-43
  2. Ferguson M, Ward DC. Cell cycle dependent chromosomal movement in pre-mitotic human T-lymphocyte nuclei. Chromosoma 1992; 101(9): 557-65
  3. Derenzini M, Farabegoli F, Pession A, Novello F. Spatial redistribution of ribosomal chromatin in the fibrillar centre of human circulating lymphocytes after stimulation of transcription. Exp Cell Res 1987; 170(1): 31-41
  4. Trere D. AgNOR staining and quantification. Micron 2000; 31(2): 127-31
  5. Hernandez-Verdun D. Nucleolus: from structure to dynamics. Histochem Cell Biol. 2006; 125(1-2): 127-37
  6. Demirtas H, Imamoglu N, Donmez H, Cucer N, Yilmaz A, Candemir Z. Condensed chromatin surface and NORs surface enhancement in mitogen-stimulated lymphocytes of Down syndrome patients. Ann Genet 2001; 44(2): 77-82
  7. Imamoglu N, Demirtas H, Donmez-Altuntas H, Ilten A. Higher NORsexpression in lymphocyte of trisomy 21 babies/children: in vivo evaluation. Micron 2005; 36(6): 503-7
  8. Demirtas H, Candemir Z, Cucer N, İmamoğlu N, Donmez H, Bokesoy I. Essay on the nucleoli survey by the and - satellite DNA probes of the acrocentric chromosomes in mitogen-stimulated human lymphocyte. Ann Genet 2000; 43(2): 61-8
  9. Strouboulis J, Wolffe AP. Functional compartimentalization of the nucleus. J Cell Sci 1996; 109(Pt 8): 1991-2000
  10. Cremer T, Kreth G, Koester H, Fink RH, Heintzmann R, Cremer M, et al. Chromosome territories, interchromatin domain compartment, and nuclear matrix: an integrated view of the functional nuclear architecture. Crit Rev Eukaryot Gene Expr 2000; 10(2): 179-212
  11. Sun HB, Shen J, Yokota H. Size-dependent positioning of human chromosomes in interphase nuclei. Biophysical J 2000; 79(1): 184-90
  12. Wang RF. Functional control of regulatory T cell and cancer immotherapy. Seminars in Cancer Biology 2006; 16(2): 106-14
  13. Ostrand-Rosenberg S. CD4+ T lymphocytes: a critical component of antitumor immunity. Cancer Invest 2005; 23(5): 413-9
  14. Gerloni M, Zanetti M. CD4 T cells in tumor immunity. Springer Semin Immunopathol 2005; 27(1): 37-48
  15. Knutson KL, Disis ML. Augmenting T helper cell immunity in cancer. Curr Drug Targets Immune Endocr Metabol Disord 2005; 5(4): 365-71
  16. Fehervari Z, Sakaguchi S. CD4+ regulatory cells as a potential immunotherapy. Philos Trans R Soc Lond B Biol Sci 2005; 360(1461): 1647-61
  17. Lopez M, Aguilera R, Perez C, Mendoza AN. The role of regulatory T Lymphocytes in the induced