SavedAdd your journal

Journals / Turkish Journal of Fisheries and Aquatic Sciences / 2013 / Cilt: 13 - Sayı: 2

Humik asitin kahverengi alabalık solungaç ve kaslarında kadmiyum toksisitesine karşı histopatolojik ve biyokimyasal etkisi

Histopathological and biochemical effects of humic acid against cadmium toxicity in brown trout gills and muscles

GONCA ALAK
MUHAMMED ATAMANALP
AHMET TOPAL
HARUN ARSLAN
ERTAN ORUÇ
SERDAR ALTUN
Pages
315–320
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results suggest that humic acid may counteract the cadmium toxicity in muscles tissue in histopathologic aspect.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:It was biochemically and histopathologically investigated whether humic acid (HA) has protective effects on cadmium (Cd) toxicity on muscle and gills of brown trout (Salmo trutta fario Linnaeus, 1792).
📊
Key Findings & Data:The brown trout were exposed to cadmium (2 ppm) and/or humic acid (5 ppm).
💡
Conclusion & Impact:These results suggest that humic acid may counteract the cadmium toxicity in muscles tissue in histopathologic aspect.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

It was biochemically and histopathologically investigated whether humic acid (HA) has protective effects on cadmium (Cd) toxicity on muscle and gills of brown trout (Salmo trutta fario Linnaeus, 1792). The brown trout were exposed to cadmium (2 ppm) and/or humic acid (5 ppm). For this purpose, levels of glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA) was investigated in muscle and gills tissues of brown trout. The activities of GPx and SOD in the tissues of fish exposed to Cd was significantly lower than the control groups (P<0.05). MDA levels did not increase in the groups exposed to cadmium (P>0.05). Hovewer, humic acid did not affect biochemical damage in cadmium group. Cd caused a significant increase in histopathological changes in muscle and gills tissues, but histopathological changes were lower in the muscle tissue of Cd+HA group. These results suggest that humic acid may counteract the cadmium toxicity in muscles tissue in histopathologic aspect.

Özet

Kadmiyum toksisitesine karşı humik asitin koruyucu etkisi biyokimyasal ve histopatolojik olarak kahverengi alabalık (Salmo trutta fario Linnaeus, 1792) solungaç ve kas dokularında araştırılmıştır. Balıklar kadmiyum ve/veya humik asite (2ppm Cd, 5 ppm humik asit) maruz bırakılmışlardır. Bu amaçla kahverengi alabalıkların kas ve solungaç dokularında glutatyon peroksidaz (GPX), süperoksit dismutaz (SOD), malondialdehit (MDA) düzeyleri incelenmiştir. Cd’a maruz kalan gruplarda GPX ve SOD enzim aktiviteleri kontrol grubuna kıyasla düşük değerler vermiştir (P 0.05). Bununla beraber kadmiyum uygulanan gruplarda humik asit biyokimyasal zararı önlemede etkili olmamıştır. Cd kas ve solungaç dokularında histopatolojik olarak önemli artışlara neden olurken, Cd+HA’in birlikte uygulandığı grupta kas dokusu daha az etkilenmiştir. Bu sonuçlara göre, humik asit kas dokusunda histopatolojik olarak koruyucu etki gösterebilir

🔖

Cite This Article

ALAK, G., ATAMANALP, M., TOPAL, A., ARSLAN, H., ORUÇ, E., ALTUN, S. (2013). Humik asitin kahverengi alabalık solungaç ve kaslarında kadmiyum toksisitesine karşı histopatolojik ve biyokimyasal etkisi. Turkish Journal of Fisheries and Aquatic Sciences, Cilt: 13 - Sayı: 2, 315–320. https://ajindex.com/articles/408085

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Alak, G., Atamanalp, M., Topal, A., Arslan, H., Kocaman, E.M., Oruç, E. 2013. Effect of sub-lethal lead toxicity on the histopathological and antioxidant enzyme activity of rainbow trout (Oncorhynchus mykiss). Fresenius Environmental Bulletin, 22: 733-738.
  2. Allen, P. 1995. Chronic accumulation of cadmium in the edible tissues of Oreochromis aureus (Steindachner): modification by mercury and lead. Archives of Environmental Contamination and Toxicology, 29: 8- 14 Amdur, M.O., Doull, J., Curtis, M.D. and Kloasen, C.D. 1991. Cassarett and Doull's Toxicology. Pergamon, New York/Oxford Anderson, D. 1996. Antioxidant defences against reactive oxygen species causing genetic and other damage. Mutation Research, 350: 103-108
  3. Andersson, C., Abrahamson, A., Brunstrom, B. and Orberg, J. 2010. Impact of humic substances on EROD activity in gill and liver of three-spined sticklebacks (Gasterosteus aculeatus). Chemosphere, 81: 156-160.
  4. Ates, B., Orun, I., Talas, Z.S., Durmaz, G. and Yilmaz, I. 2008. Effects of sodium selenite on some biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) exposed to Pb2+ and Cu2+. Fish Physiology Biochemistry, 34:53- 59. Aydın, A., Alak, G., Kocaman, E.M. and Atamanalp, M. 2012. Effects of Carboxin on Glutathione-S- Transferase Enzyme Activite in Rainbow Trout (Oncorhynchus mykiss). Journal of Animal and Veterinary Advances, 11(15): 2716-2720.
  5. Besirovic, H., Alic, A., Prasovic, S., Drommer W. 2010. Histopathological Effects of Chronic Exposure to Cadmium and Zinc on Kidneys and Gills of Brown Trout (Salmo trutta fario) Turkish Journal of Fisheries and Aquatic Sciences, 10: 255-262.
  6. Bingöl, S.A. and Kocamiş, H., 2010. The Gene Expression Profile by RT-PCR and Immunohistochemical Expression Pattern of Catalase in the Kidney Tissue of Both Healthy and Diabetic Mice. Kafkas Univ Vet Fak Derg, 16 (5): 825-834,
  7. Bradford, M.M. 1976. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochemistry, 72: 248-254.
  8. Cankaya, M., Hernandez, A.M., Ciftci, M., Beydemir, S., Ozdemir, H., Budak, H., Gulcin, I., Comakli, V., Emircupani, T., Ekinci, D., Kuzu, M., Jiang, O., Eichele, G. and Kufrevioglu, O.I. 2007. An analysis of expression patterns of genes encoding proteins with catalytic activities. Genomics, 8: 232-248.
  9. Ceyhun, S.B., Şenturk, M., Ekinci, D., Erdogan, O., Ciltas, A. and Kocaman, E.M. 2010. Deltamethrin attenuates antioxid