Journals / Turkish Journal of Fisheries and Aquatic Sciences / 2016 / Cilt: 16 - Sayı: 4

Rus Mersin Balığının, Acipencer gueldenstaedtii Brandt ve Ratzenburg, 1833, Yaşamlarının İlk Dönemlerindeki Proteaz Aktivitesi Üzerine Bir Ön Çalışma

A Preliminary Study on Protease Activity of Russian Sturgeon, Acipencer gueldenstaedtii Brandt and Ratzenburg, 1833, at Early Life Stages

Pages
1025–1029
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Additionally, dual combinations between fish meal and soybean meal/soybean protein concentration with the ration of three to one (3:1) was moderate (26.38 and 22.13 %), whereas blood meal extremely produced a 97.28% inhibitory ratio

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The fish feed industry continues to researches for optimum diet demands for candidate species culture.
📊
Key Findings & Data:In the first step, feeding with live prey and transition period to artificial feed significantly affected the daily amount of protease in digestive system (P<0.05).
💡
Conclusion & Impact:Additionally, dual combinations between fish meal and soybean meal/soybean protein concentration with the ration of three to one (3:1) was moderate (26.38 and 22.13 %), whereas blood meal extremely produced a 97.28% inhibitory ratio
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The fish feed industry continues to researches for optimum diet demands for candidate species culture. For this purpose, in vitro analyze methods may be more efficient than in vivo assays. This study includes two different stages; the first one is about the effect of commercial feeding protocol on protease enzyme alteration and the second one is about the inhibitory effects of different protein sources on early life proteases of Russian sturgeon, A. gueldenstaedtii, juveniles. In the first step, feeding with live prey and transition period to artificial feed significantly affected the daily amount of protease in digestive system (P<0.05). In the second part, some protein sources used in micro diets were tested in vitro for examination of their possible inhibitory effects on the proteases of Russian sturgeon larvae. The minimum inhibitory effect was obtained from fish meal (15.44%), but however, soybean protein concentration, soybean meal, corn gluten and rice bran inhibited the proteases significantly higher than FM and its combinations (63.55, 71.81, 72.24, and 80.77%, respectively). Additionally, dual combinations between fish meal and soybean meal/soybean protein concentration with the ration of three to one (3:1) was moderate (26.38 and 22.13 %), whereas blood meal extremely produced a 97.28% inhibitory ratio

Özet

Balık yemi endüstrisi, aday türlerin optimum rasyon ihtiyacını belirlemek için araştırmalarına devam etmektedir. Bu amaçla yapılan in vitro analiz metotları, in vivo metotlara göre daha verimli olabilir. Bu çalışma iki farklı çalışmadan oluşmaktadır; bunlardan birincisi ticari besleme protokolünün proteaz enzimlerinin değişimi üzerine olan etkisini, ikinci çalışma ise farklı protein kaynaklarının Rus mersini, A. gueldenstaedtii, jüvenillerinin erken dönem proteaz aktivitiesi üzerine olan inhibisyon etkisini belirlemeyi amaçlamıştır. İlk çalışmada, canlı yem ile besleme ve karma yeme geçiş periyodunun, sindirim sistemindeki günlük proteaz miktarını istatistiki olarak etkilediği tespit edilmiştir (P<0.05). İkinci çalışmada ise, mikro yemlerde kullanılan bazı protein kaynakları, Rus mersini jüvenillerinin proteaz enzimlerini olası inhibe edici etkilerini belirlemek için in vitro olarak test edilmiştir. En düşük inhibisyon etkisi balık ununda (%15.44) gözlemlenmiş, ancak buna karşın soya protein konsatresi, soya unu, mısır glüten ve pirinç kepeği proteaz enzimlerini balık unu ve diğer hammaddelerle yaptığı kombinasyonlardan istatistiki açıdan daha yüksek oranda inhibe etmiştir (sırasıyla % 63.55, 71.81, 72.24 ve 80.77). Buna ek olarak, balık ununun soya unu/soya protein konsantresi ile 1:3 oranındaki kombinasyonları kabul edilebilir seviyede inhibe edici bulunurken (%26.38 ve 22.13), kan ununun aşırı seviyede inhibe edici olduğu tespit edilmiştir (%97.28)

🔖

Cite This Article

GÖKÇEK, C. K., SZABÓ, T. (2016). Rus Mersin Balığının, Acipencer gueldenstaedtii Brandt ve Ratzenburg, 1833, Yaşamlarının İlk Dönemlerindeki Proteaz Aktivitesi Üzerine Bir Ön Çalışma. Turkish Journal of Fisheries and Aquatic Sciences, Cilt: 16 - Sayı: 4, 1025–1029. https://ajindex.com/articles/407623

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (7)

  1. Alarcon, F. J., Moyano, F. J., Diaz, M., Fernandez-Diaz, C., Yufera, M., 1999. Optimization of the protein fraction of microcapsules used in feeding of marine fish larvae using in vitro digestibility techniques. Aquaculture Nutrition, 2095.1999.00093.x doi: 10.1046/j.1365 Alptekin, C., Gökçek, K., 2016. Balık Yemlerinde Kullanılan Farklı Protein Kaynaklarının Sibirya Mersini (Acipencer baerii Brandt 1869) Jüvenillerinin Proteaz Aktivitesi Üzerine Etkileri. Yunus Araştırma Bülteni, doi:10.17693/yunus.32866 Babaei, S. S., Kenari, A. A., Nazari, R., Gisbert, E., 2011. Developmental changes of digestive enzymes in Persian Sturgeon (Acipencer percius) during larval ontogeny. doi:10.1016/j.aquaculture.2011.04.032 318: 138-144.
  2. Bradford, M. M. 1976. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dry binding. Anal. Biochem., 72, 248-254.
  3. Cahu, C. L. and Zambonino, J. L., 1995. Maturation of the pancreatic and intestinal digestive functions in sea bass (Dicentrarchus labrax): effect of weaning with different protein sources. Fish Physiol. Biochem., 14: 431-437.
  4. Cho, C.Y. 1987. La energia en la nutricion de los peces. In: Nutricion en Acuicultura, vol. II (ed. By de la Monteros Espinosa nad Lanarta U.), pp. 197-244.
  5. CAICYT, Madrid, Spain. Dimes, L. E., Haard, N. F., Dong, F. M., Rasco, B. A., Forster, J. P., Fairgrieve, W. T., Arndt, R., Hardy, R. W., Barrows, F. T., Higs, D. A., 1994. Estimation of protein digestibility. II. In vitro assay of protein in salmonid feeds. Comp. Biochem. Physiol. 108A: 363- 370. doi:10.1016/0300-9629(94)90107-4
  6. Ezquerra, J. M., 1997. Enzyme technology in aquaculture: in vitro evaluation of enzymatic digestibility of dietary proteins by pH-stat for the optimization of diets for cultured Penaeus vannamei. Ph.D Thesis, CIBNOR, La Paz, Mexico. Faulk, C. K., Benninghoff, A. D., Holt, G. J., 2007. Ontogeny of the gastrointestinal tract and selected digestive enzyme in cobia Rachycentron canadum (L.). J. Fish Biol. 70:567-583. doi: 10.1111/j.1095- 8649.2007.01330.x Furne, M., Hidalgo, M. C., Lopez, A., Garcia- Gallego, M., Morales, A. E., Domezain, A., Domezaine, J., Sanz, A., 2005. Digestive enzyme activities in Adriatic sturgeon Acipencer naccarii and rainbow trout Oncorhynchus Aquaculture, doi:10.1016/j.aquaculture.2005.05.017 study. 391-398.
  7. Garcia-Carreno, F. L., 1996. Proteinase inhibitors. Trends Food Sci. Technol., 7: 197-204. doi:10.1016/0924- 2244(96)10023-6 Gawl