Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 2008 / Cilt: 19 - Sayı: 1

The Diagnosis of Bacterial Kidney Disease (BKD) From Cultured Rainbow Trout (Oncorhynchus Mykiss)

Kültürü Yapılan Gökkuşağı Alabalıklarında (Oncorhynchus Mykiss) Bakteriyel Böbrek Hastalığı (BKD)’nın teşhisi

Pages
1–8
Publication Date
2008-06-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In our laboratories, Bacterial Kindey Disease could be diagnosed rapidly by conventional isolation and identification with DFAT and nested-PCR on rainbow trout.

📌 3 Key Takeaways

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Objective & Hypothesis:In this project, totally 360 samples from 5 rainbow trout farms on Kesikköprü barrage lake and aseptically 120 kidney tissues about 6-12 months rainbow trout from one rainbow trout farm from 2005 to 2007 were collected for isolation and identification of R.salmoninarum;.
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Key Findings & Data:Ovarian (coelemic) fluids were collected from 120 mature females of fish farming on Safranbolu.
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Conclusion & Impact:In our laboratories, Bacterial Kindey Disease could be diagnosed rapidly by conventional isolation and identification with DFAT and nested-PCR on rainbow trout.
🔬Design:Research Article
📍Scope:2005 · 360 samples
⚙️Variables:Etlik Veterinerlik Mikrobiyoloji Dergisi · Kültür · Gökkuşağı Balıkları

Abstract

In this project, totally 360 samples from 5 rainbow trout farms on Kesikköprü barrage lake and aseptically 120 kidney tissues about 6-12 months rainbow trout from one rainbow trout farm from 2005 to 2007 were collected for isolation and identification of R.salmoninarum;. Ovarian (coelemic) fluids were collected from 120 mature females of fish farming on Safranbolu. KDM-2 medium was used for isolation from samples. R.salmoninarum was not isolated on totally 600 samples. DFAT and nested- PCR results of all samples were negative. As a result Bacterial Kindey Disease was not detected in Ankara and Safranbolu. In our laboratories, Bacterial Kindey Disease could be diagnosed rapidly by conventional isolation and identification with DFAT and nested-PCR on rainbow trout.

Özet

Bu projede Renibacterium salmoninarum izolasyon ve identifikasyonu için, 2005-2007 yılları arasında, Kesikköprü Baraj Göleti’nde yer alan 5 adet alabalık işletmesinden toplam 360 adet; Safranbolu’da yer alan 1 adet alabalık işletmesinden 120 adet 6-12 aylık alabalıklardan böbrek örnekleri aseptik koşullarda alındı. Safranbolu’da yer alan 1 adet alabalık işletmesinde 120 adet olgun dişi balıklardan ovaryum sıvısı uygun koşullarda alındı. Toplanan örneklerden izolasyon için KDM-2 medium kullanıldı. Toplam 600 adet örnekten hiç R.salmoninarum izolasyonu olmadı. Aynı örneklerden DFAT ve nested-PCR testleri yapıldı. Örneklerin hiçbirinde etkene rastlanılamadı. Sonuç olarak Ankara ve Safranbolu’da Bakteriyel Böbrek Hastalığı saptanamamıştır. Laboratuarımızda alabalıklarda konvansiyonel izolasyon ve identifikasyon yanında DFAT ve nested-PCR gibi testlerle Bakteriyel Böbrek Hastalığı kısa sürede teşhis edilebilir hale gelmiştir.

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Cite This Article

ÖZKÖK, S., ŞEN, S., ÜN, H. (2008). The Diagnosis of Bacterial Kidney Disease (BKD) From Cultured Rainbow Trout (Oncorhynchus Mykiss). Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 19 - Sayı: 1, 1–8. https://ajindex.com/articles/406392

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