SavedAdd your journal

Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 2014 / Cilt: 25 - Sayı: 1

Detection of bovine Partuberculosis with ELISA and fecal examination

ELISA ve fekal bakteriyoskopi ile sığırlarda Paratuberküloz prevalansının belirlenmesi

A.ebru BORUM
Sertan ÇATIK
Zafer MECİTOĞLU
Gülşah DEMİR
Mihriban ÜLGEN
Sezgin ŞENTÜRK
Pages
1–5
Publication Date
2014-12-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The prevalence of paratuberculosis in dairy cows, Ziehl-Neelsen staining with 4.59% in fecal samples, 31.80% positive by the ELISA test were determined.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, 305 between 4-8 years on a farm in Holstein Fresian dairy cows in Afyon were used.
📊
Key Findings & Data:In two of these animals with chronic diarrhea were present and cows did not respond to treatment.
💡
Conclusion & Impact:The prevalence of paratuberculosis in dairy cows, Ziehl-Neelsen staining with 4.59% in fecal samples, 31.80% positive by the ELISA test were determined.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Sezgin ŞENTÜRK · Mihriban ÜLGEN · Zafer MECİTOĞLU

Abstract

In this study, 305 between 4-8 years on a farm in Holstein Fresian dairy cows in Afyon were used. In two of these animals with chronic diarrhea were present and cows did not respond to treatment. Clinical studies, hematological parameters were determined, and blood serum and fecal samples were taken in other cattle in the herd. Mycobacterium avium subsp. Paratuberculosis (MAP) in serum samples was determined using ELISA. The prevalence of paratuberculosis in dairy cows, Ziehl-Neelsen staining with 4.59% in fecal samples, 31.80% positive by the ELISA test were determined.

Özet

Bu çalışmada Afyon ve civarındaki çiftliklerden 4-8 yaş arasındaki 305 Holstein Fresian sütçü sığırlar kullanılmıştır. Bu sığırların iki tanesinde kronik diare şikayeti mevcuttu ve yapılan tedaviye cevap vermemiştir. Sürüdeki diğer sığırlarda klinik inceleme yapılmış, hematolojik parametrelere bakılmış, teşhis amaçlı dışkı ve kan serumu örnekleri alınmıştır. Mycobacterium avium subsp. paratuberculosis (MAP) serum örneklerinde ELISA yöntemi kullanılarak tespit edildi. Süt sığırlannda paratuberküloz’un prevalansı, dışkı örneklerinde; Ziehl-Neelsen boyama ile %4.59, ELISA testi ile ise %31.80 oranında pozitif tespit edilmiştir

🔖

Cite This Article

BORUM, A., ÇATIK, S., MECİTOĞLU, Z., DEMİR, G., ÜLGEN, M., ŞENTÜRK, S. (2014). Detection of bovine Partuberculosis with ELISA and fecal examination. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 25 - Sayı: 1, 1–5. https://ajindex.com/articles/406381

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Abendan N, Sevilla IA, Prieto JM, Garrido JM, Juste RA, Alonso-Hearn M, (2013). Mycobacterium aviumsubspeciesparatuberculosis isolates from sheep and goats Show reduced persistence in bovine macrophages than cattle,bison, deer and wild boar strains regardless of genotype. Vet Microbiol. 163, 325-334.
  2. Antognoli MC, Hirst HL, Garry FB, Salman MD, (2007). Immune Response to and Faecal Shedding of Mycobacterium avium ssp. paratuberculosis in Young Dairy Calves, and the Association Between Test Results in the Calves and the Infection Status of their Dams. Zoonoses Public Hlth. 54, 152-159. Collins MT, and Sockett DC, (1993a). Accuracy and economics of USDA-Licensed enzyme-linked immunosorbent assay for bovine paratuberculosis. J Am Vet Med Assoc. 203, 1456-1463.
  3. Collins MT, Sockett DC, Ridge F, (1993b). Evaluation of a commercial enzyme-linked immunosorbent assay for Johne’s disease. J Clin Microbiol. 5, 52-55.
  4. Collins MT, Gardner IA, Garry FB, Roussel AJ, Wells JS, (2006). Consensus recommendations on diagnostic testing for the detection of paratuberculosis in cattle in the United States. JAVMA-J AM VET MED A 229(12), 1912-1919.
  5. Çetinkaya B, Erdoğan HM, Morgan KL, (1997). Risk factors for Bovine Paratuberculosis. II. The multiple analysis of risk ractors for Bovine Paratuberculosis. Turk J Vet Anim Sci. 21, 303-306.
  6. Çetinkaya B, Muz A, Ertaş HB, Öngör H, Sezen İY, Gülcü HB, (2000). Süt ineklerinde Paratüberküloz Prevalansının Polimeraz Zincir Reaksiyonu (PZR) ile Saptanması. Turk J Vet Anim Sci. 24, 371-379.
  7. De Kantor IN, Weyer KE, (2004). 1998-last update,laboratory services in tuberculosis control, part II. Dieguez FJ, Gonzalez AM, Menendez S, Vilara MJ, Sanjuana ML, Yus E, Arnai I, (2009). Evaluation of four commercial serum ELISAs for detection of Mycobacterium avium subsp. paratuberculosis infection in dairy cows. Vet J. 180(2), 2231-235. Fujiki A, (2001). Direct smear examination. In: TB Bacteriology Examination to Stop TB. The Research Institute of Tuberculosis, ed. Fujiki A, Japan International Cooperation Agency JINNOU Co. 7. Garcia RR, Perez-de-la-Lastra JM, Vicente J, Ruiz- Fons F, Garrido JM, Gortazar C, (2008). Large-scale ELISA testing of Spanish red deer for paratuberculosis. Vet Immunol Immunopathol.124, 75-81.
  8. Gasteiner J, Wenzli H, Fuchs K, Jark U, Baumgartner W, (1999). Serological Cross-sectional Study of Paratuberculosis in Cattle in Austria. J Vet Med. B 46, 457-466.
  9. Gilardoni LR, Paolıcchi FA, Mundo SL. (2012). Bovine paratuberculosis