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Journals / Turkish Journal of Pharmaceutical Sciences / 2007 / Cilt: 4 - Sayı: 1

Biyolojik aktiviteyle yönlendirilen fraksiyonlama ile Cirsium hypoleucum dc.’dan elde edilen iki flavonol glikozitin antioksidan aktivitesi

Antioxidant activity of two flavonol glycosides from Cirsium hypoleucum dc. through bioassay-guided fractionation

DİDEM DELİORMAN ORHAN
FATMA ERGÜN
ERDEM YEŞİLADA
Kochiro TSUCHIYA
Yoshihisa TAKAISHI
Kazuyoshi KAWAZOE
Pages
1–14
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Flavonoid 1 showed more potent antioxidant activity than flavonoid 2

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The flowers, roots and stems of Cirsium species are used as a folk remedy in Anatolia.
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Key Findings & Data:The bioassay guided fractionation of methanol extract led to the isolation flavonoid 1 and 2 from n–butanol fr.
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Conclusion & Impact:Flavonoid 1 showed more potent antioxidant activity than flavonoid 2
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Elektron spin rezonans spektroskopi · Bitkiler · tıbbi

Abstract

The flowers, roots and stems of Cirsium species are used as a folk remedy in Anatolia. In order to evaluate antioxidant activity of Cirsium hypoleucum which is an endemic plant, superoxide radical (hypoxanthine-xanthine oxidase system) and free radical scavenging activities (DPPH) using in vitro electron spin resonance (ESR) spectrometry of the different extracts, fractions and compounds obtained by Bioassay Guided Fractionation (BAGF) technique were studied. The bioassay guided fractionation of methanol extract led to the isolation flavonoid 1 and 2 from n–butanol fr. The structures of these compounds were elucidated using UV, IR, MS, 1H- and 13C-NMR techniques as quercetin-3-O-rutinoside (rutin) (1) and kaempferol-3-O-rutinoside (nicotiflorin) (2). Flavonoid 1 showed more potent antioxidant activity than flavonoid 2

Özet

Cirsium türlerine ait çiçekler, dallar ve kökler Anadolu’da halk ilacı olarak kullanılmaktadır. Endemik bir bitki olan Cirsium hypoleucum’un antioksidan aktivitesini değerlendirmek için, biyolojik aktivite ile yönlendirilen fraksiyonlama (BAYF) tekniği ile elde edilen ekstre, fraksiyon ve bileşiklerin süperoksit radikali (hipoksantin-ksantin oksidaz sistemi) ve serbest radikal süpürücü aktiviteleri (DPPH) in vitro elektron spin rezonans (ESR) tekniği ile incelenmiştir. Metanol ekstresinin biyolojik aktivite yönlendirmeli fraksiyonlanması neticesinde n-butanol fraksiyonundan flavonoit 1 ve 2 izole edilmiştir. Bu bileşiklerin yapıları UV, IR, MS, 1H- ve 13C-NMR teknikleri kullanılarak kersetin-3-O-rutinozit (rutin) (1) ve kemferol-3-O-rutinozit (nikotiflorin) (2) olarak tayin edilmiştir. Rutin, nikotiflorine göre daha güçlü bir antioksidan etki göstermiştir.

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Cite This Article

ORHAN, D. D., ERGÜN, F., YEŞİLADA, E., TSUCHIYA, K., TAKAISHI, Y., KAWAZOE, K. (2007). Biyolojik aktiviteyle yönlendirilen fraksiyonlama ile Cirsium hypoleucum dc.’dan elde edilen iki flavonol glikozitin antioksidan aktivitesi. Turkish Journal of Pharmaceutical Sciences, Cilt: 4 - Sayı: 1, 1–14. https://ajindex.com/articles/403889

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