SavedAdd your journal

Journals / Turkish Journal of Pharmaceutical Sciences / 2010 / Cilt: 7 - Sayı: 3

Ratlarda radyasyonla indüklenen lens epitel doku hasarma karşı verapamil’in koruyucu etkilerinin değerlendirilmesi

Evaluation of protective effects of verapamil against lens epithelial tissue injury induced by irradiation in rats

Kadir BATÇIOĞLU
Haldun Ş. ERKAL
Meltem SERİN
A. Burçin UYUMLU
Neslihan YÜCEL
Battal YILDIRIM
BASRİ SATILMIŞ
Pages
267–272
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, we can suggest that verapamil as a protectant agent against radiation induced lens epithelial tissue injury.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, we investigated the possible protectant effects of verapamil against lens epithelial tissue injury induced by irradiation in rats.
📊
Key Findings & Data:Forty female Wistar Albino rats were divided into four groups (n=10).
💡
Conclusion & Impact:In conclusion, we can suggest that verapamil as a protectant agent against radiation induced lens epithelial tissue injury.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, we investigated the possible protectant effects of verapamil against lens epithelial tissue injury induced by irradiation in rats. Forty female Wistar Albino rats were divided into four groups (n=10). Group 1 received 10 mg/kg verapamil and underwent 20 Gy irradiation. Group 2 received no verapamil and underwent 20 Gy irradiation. Group 3 received 10 mg/kg verapamil and underwent sham irradiation. Group 4 received no verapamil and underwent sham irradiation. The lens tissues were dissected and measured malondialdehyde levels in all groups. The mean malondialdehyde levels (nmol/mg prt) were determined as 5,59 for Group 1, 7,04 for Group 2, 3,95 for Group 3 and 3,25 for Group 4. In conclusion, we can suggest that verapamil as a protectant agent against radiation induced lens epithelial tissue injury.

Özet

Bu çalsmada, ratlarda radyasyonla indüklenen lens epitel hücre hasarna karsi verapamilin olası koruyucu etkisini inceledik. Kırk adet dişi Wistar Albino türü rat dört guruba ayrıldı (n=10). Gurup 1’e 20 Gy dozda radyasyon ve 10 mg/kg verapamil uygulandı. Gurup 2’ye 20 Gy radyasyon uygulandı ve verapamil verilmedi. Gurup 3’e 10 mg/kg dozda verapamil verildi, yalancı radyasyon uygulandı. Gurup 4’e verapamil verilmedi ve yalancı radyasyon uygulandı. Tüm guruplarda lens dokular ç ikarldı ve malondialdehit (MDA) ölçümleri yapıldı. Ortalama MDA düzeyleri (nmol/mg prt); Gurup 1 için 5.59, Gurup 2 için 7.04, Gurup 3 için 3.95 ve Gurup 4 için 3.25 olarak belirlendi. Sonuç olarak, radyasyonla indüklenen lens epitel hücre hasarna karsi verapamili koruyucu bir ajan olarak önerebiliriz.

🔖

Cite This Article

BATÇIOĞLU, K., ERKAL, H. Ş., SERİN, M., UYUMLU, A. B., YÜCEL, N., YILDIRIM, B., SATILMIŞ, B. (2010). Ratlarda radyasyonla indüklenen lens epitel doku hasarma karşı verapamil’in koruyucu etkilerinin değerlendirilmesi. Turkish Journal of Pharmaceutical Sciences, Cilt: 7 - Sayı: 3, 267–272. https://ajindex.com/articles/403796

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Ertekin, M.V., Koçer. I., Karslioğlu, I., Taysi, S., Gepdiremen, A., Sezen, O., “Effects of oral Gingko biloba supplementation on cataract formation and oxidative stress occuring in lenses of rats exposed to total cranium radiotherapy” Jpn. J. Ophthalmol., 48, 5, 499-502, 2004
  2. Orhan Deliorman, D., Ergun, F., Yesilada, E., Tsuchiya, K., Takaishi, Y., Kawazoe, K., “Antioxidant activity of two flavanol glycosides from Cirsium hypoleucum DC. through bioassay-guided fractionation” Turk J. Pharm. Sci., 4, 1, 1-14, 2007
  3. Halliwell, B., Gutteridge, J.M., “Oxygen toxicity, oxygen radicals, transition metals and disease” Biochem. J., 219, 1-14, 1984
  4. Colkesen, A., Aydin, A., Isimer, A., Orhan, I., Sener, B., “Comparative free radical scavenging capacity of the seed extracts obtained from the white and red grape berries used wine-making in Turkey” Turk J. Pharm. Sci., 3, 3, 177-85, 2006
  5. Iwig, M., Glaesser, D., Fass, U., Struck, H.G., “ Fatty acid cytotoxicity to human lens epithelial cells” Exp. Eye Res. 79: 689-704, 2004
  6. Bhuyan, K.C., Master, R.W., Coles, R.S., Bhuyan, D.K., “ Molecular mechanisms of cataractogenesis: evidence of phospholipid malondialdehyde adduct in human senile cataract” Mech. Ageing Dev. 34, 289-296, 1986
  7. Cengiz, M., Gurkaynak, M., Atahan, I.L., Kilic, K., Totan, Y., “The effect of verapamil in the prevention of radiation-induced cataract” Int.J. Radiat. Oncol. Biol. Phys., 43, 623-626, 1999
  8. Mihara, M., Uchiyama, M., “ Determination of malonaldehyde precursor in tissues by thiobarbituric acid test” Anal. Biochem., 86, 271-278, 1978
  9. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., “ Protein measurement with the folin phenol reagent” J. Biol. Chem., 193, 265-275, 1951
  10. Johar, S.R., Rawal, U.M., Jain, N.K., “Vasavada AR. Sequential effects of ultraviolet radiation on the histomorphology, cell density and antioxidative status of the lens epithelium: An in vivo study” Photochem. Photobiol. 78, 306-11, 2003
  11. Comporti, M., “ Lipid peroxidation: Biopathological significance” Mol. Aspects Med., 14, 199-207, 1993
  12. Srivastava, A.K., Goel, U.C., Bajaj, S., Singh, K.J., Dwivedi, N.C., Tandon, M.P.,“Visual evoked responses in ethambutol induced optic neuritis” J. Assoc. Physicians Ind., 45, 847-849, 1997
  13. Sanderson, J., Marcantonio, J.M., Duncan, G., “Calcium ionophore induced proteolysis and cataract: Inhibition by cell permeable calpain antagonists” Biochem. Biophys. Res. Commun., 218, 893-901, 1996