Journals / Turkish Journal of Pharmaceutical Sciences / 2017 / Cilt: 14 - Sayı: 2

Evaluation of Antioxidant Activities and Phenolic Compounds of Scorzonera latifolia (Fisch. & Mey.) DC. Collected from Different Geographic Origins in Turkey

Türkiye'nin Farklı Coğrafik Bölgelerinden Toplanmış Scorzonera latifolia (Fisch. & Mey.) DC. Örneklerinin Fenolik Bileşiklerinin ve Antioksidan Aktivitelerinin Değerlendirilmesi

Pages
179–184
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

MDA level was detected as the lowest with treatment of S. latifolia Bayburt root sample (4.41 nmol/mL).Conclusion: According to the antioxidant activity results, no significant difference was observed in the antioxidant potential between the samples collected from different locations except for S. latifolia collected from the Kars region.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: The chemical composition of plants is considered to be affected by many parameters.
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Key Findings & Data:In the present study, evaluation of antioxidant activity potentials of Scorzonera latifolia (Fisch. & Mey.) DC. aerial parts and roots, which were collected from different regions of Turkey, was aimed.Materials and Methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and measurement of malondialdehyde (MDA) levels were used for determining antioxidant capacities of the tested extracts.
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Conclusion & Impact:MDA level was detected as the lowest with treatment of S. latifolia Bayburt root sample (4.41 nmol/mL).Conclusion: According to the antioxidant activity results, no significant difference was observed in the antioxidant potential between the samples collected from different locations except for S. latifolia collected from the Kars region.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: The chemical composition of plants is considered to be affected by many parameters. Therefore, the region where the samples are collected is likely to have an influence on the composition of phenolic compounds, so that their biological activities. In the present study, evaluation of antioxidant activity potentials of Scorzonera latifolia (Fisch. & Mey.) DC. aerial parts and roots, which were collected from different regions of Turkey, was aimed.Materials and Methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and measurement of malondialdehyde (MDA) levels were used for determining antioxidant capacities of the tested extracts. In order to observe variations in the chemical composition of the investigated samples qualitatively as well as quantitatively, high performance liquid chromatography analyses were performed. Results: Quantitative analysis showed that the amounts of chlorogenic acid and hyperoside in plants vary according to the regions where the samples were collected. As a result aerial parts of the S. latifolia collected from the Kars region have found to contain higher amount of chlorogenic acid (1246.78±3.20 ?g/g) as well as hyperoside (652.32±2.48 ?g/g) than other samples. The highest DPPH radical scavenging activity was determined with the IC value of 1.036 mg/mL for S. latifolia aerial parts of Kayseri sample. MDA level was detected as the lowest with treatment of S. latifolia Bayburt root sample (4.41 nmol/mL).Conclusion: According to the antioxidant activity results, no significant difference was observed in the antioxidant potential between the samples collected from different locations except for S. latifolia collected from the Kars region.

Özet

Amaç: Bitkilerin kimyasal içeriği pek çok parametreden etkilenmektedir. Bitkinin toplandığı bölge içerdiği fenolik bileşiklerin kompozisyonunu ve dolayısıyla aktivitelerini etkilemektedir. Bu çalışmada, Türkiye'nin farklı bölgelerinden toplanan Scorzonera latifolia (Fisch & Mey.) DC. kök ve toprak üstü kısımlarının antioksidan etki potansiyallerinin ölçülmesi amaçlanmıştır. Gereç ve Yöntemler: Test edilen ekstrelerin antioksidan kapasiteleri 1,1-difenil-2-pikrilhidrazil (DPPH) radikal süpürücü etkileri ve malondialdehit (MDA) seviyesi üzerine etkilerinin ölçülmesiyle tespit edilmiştir. Araştırma konusu olan örneklerin kimyasal içeriğindeki değişiklikleri incelemek için kalitatif ve kantitatif yüksek performanslı sıvı kromatografisi analizleri yapılmıştır. Bulgular: Kantitatif analiz sonuçları bitkilerde tespit edilen klorojenik asit ve hiperozit miktarlarının toplandığı bölgeye göre değiştiğini göstermiştir. S. latifolia Kars örneğinin diğer örneklere göre daha yüksek hiperozit (652.32±2.48 µg/g) ve klorojenik asit (1246.78±3.20 µg/g) içeriğine sahip olduğunu ortaya koymuştur. En yüksek DPPH radikal süpürücü etki 1.036 mg/mL IC50 değeri ile S. latifolia Kayseri örneğinde gözlenmiştir. En düşük MDA seviyesi ise S. latifolia Bayburt kök örneğinde (4.41 nmol/mL) belirlenmiştir. Sonuç: Antioksidan aktivite sonuçları değerlendirildiğinde, farklı lokasyonlardan toplanan örnekler arasında, S. latifolia Kars kök örneği haricinde belirgin bir farklılık tespit edilememiştir.

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Cite This Article

ACIKARA, Ö. B., ÖZ, B. E., ATEŞ, F. B., ÇİTOĞLU, G. S., NEBİOĞLU, S. (2017). Evaluation of Antioxidant Activities and Phenolic Compounds of Scorzonera latifolia (Fisch. & Mey.) DC. Collected from Different Geographic Origins in Turkey. Turkish Journal of Pharmaceutical Sciences, Cilt: 14 - Sayı: 2, 179–184. https://ajindex.com/articles/403670

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