SavedAdd your journal

Journals / Turkish Journal of Medical Sciences / 2013 / Cilt: 43 - Sayı: 5

Influence of alpha lipoic acid on epithelial apoptosis in experimental periodontitis

Adem KARA
Sümeyra AKMAN
Tevfik DEMİRCİ
Taner ARABACI
Pages
747–755
Publication Date
2013-05-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Immunohistochemical results showed that apoptotic activity decreased in the periodontitis group. 8-OHdG reactivity increased in the ALA-administered group.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Alpha lipoic acid (ALA) is a powerful antioxidant.
📊
Key Findings & Data:Materials and methods: Thirty male Wistar rats were randomly divided into 3 groups: control (without ligature), PED (ligature + placebo, saline; oral gavage), and ALA+PED (ligature + alpha lipoic acid at 50 mg/kg/day in saline; oral gavage).
💡
Conclusion & Impact:Immunohistochemical results showed that apoptotic activity decreased in the periodontitis group. 8-OHdG reactivity increased in the ALA-administered group.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Experimental periodontitis · apoptosis · alpha lipoic acid

Abstract

Alpha lipoic acid (ALA) is a powerful antioxidant. Its antiinflammatory effects and apoptotic mechanism have recently been studied in many diseases. This study aimed to investigate the effects of ALA on apoptotic activity in the treatment of periodontitis. Materials and methods: Thirty male Wistar rats were randomly divided into 3 groups: control (without ligature), PED (ligature + placebo, saline; oral gavage), and ALA+PED (ligature + alpha lipoic acid at 50 mg/kg/day in saline; oral gavage). Histological and biochemical analyses were performed on the mandibular first molars of sacrificed rats. Results: Biochemical myeloperoxidase activity in gingivo-mucosal tissue was measured. For immunohistochemical evaluation, tissue sections were stained with Bax, Bcl-2, proliferating cell nuclear antigen (PCNA), and 8-OHdG antibodies by streptavidin-biotin-peroxidase staining. Immunohistochemical results showed that apoptotic activity decreased in the periodontitis group. 8-OHdG reactivity increased in the ALA-administered group. Conclusion: ALA exhibited proapoptotic and antiinflammatory effects on gingivo-mucosal epithelium in the periodontitis.

Özet

Alpha lipoic acid (ALA) is a powerful antioxidant. Its antiinflammatory effects and apoptotic mechanism have recently been studied in many diseases. This study aimed to investigate the effects of ALA on apoptotic activity in the treatment of periodontitis. Materials and methods: Thirty male Wistar rats were randomly divided into 3 groups: control (without ligature), PED (ligature + placebo, saline; oral gavage), and ALA+PED (ligature + alpha lipoic acid at 50 mg/kg/day in saline; oral gavage). Histological and biochemical analyses were performed on the mandibular first molars of sacrificed rats. Results: Biochemical myeloperoxidase activity in gingivo-mucosal tissue was measured. For immunohistochemical evaluation, tissue sections were stained with Bax, Bcl-2, proliferating cell nuclear antigen (PCNA), and 8-OHdG antibodies by streptavidin-biotin-peroxidase staining. Immunohistochemical results showed that apoptotic activity decreased in the periodontitis group. 8-OHdG reactivity increased in the ALA-administered group. Conclusion: ALA exhibited proapoptotic and antiinflammatory effects on gingivo-mucosal epithelium in the periodontitis.

🔖

Cite This Article

KARA, A., AKMAN, S., DEMİRCİ, T., ARABACI, T. (2013). Influence of alpha lipoic acid on epithelial apoptosis in experimental periodontitis. Turkish Journal of Medical Sciences, Cilt: 43 - Sayı: 5, 747–755. https://ajindex.com/articles/398196

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Chapple ILC. Role of free radicals and antioxidants in the pathogenesis of the inflammatory periodontal diseases. J Clin Pathol-Cl Mol 1996; 49: M247–M55. Katsuragi H, Ohtake M, Kurasawa I, Saito K. Intracellular production and extracellular release of oxygen radicals by PMNs and oxidative stress on PMNs during phagocytosis of periodontopathic bacteria. Odontology 2003; 91: 13–8.
  2. Sakallioglu U, Aliyev E, Eren Z, Aksimsek G, Keskiner I, Yavuz U. Reactive oxygen species scavenging activity during periodontal mucoperiosteal healing: An experimental study in dogs. Arch Oral Biol 2005; 50: 1040–6.
  3. Koulouri O, Lappin DF, Radvar M, Kinane DF. Cell division, synthetic capacity and apoptosis in periodontal lesions analysed by in situ hybridisation and immunohistochemistry. J Clin Periodontol 1999; 26: 552–9.
  4. Canakci CF, Cicek Y, Yildirim A, Sezer U, Canakci V. Increased levels of 8-hydroxydeoxyguanosine and malondialdehyde and its relationship with antioxidant enzymes in saliva of periodontitis patients. European J of Dentistry 2009; 3: 100. Keleş GC, Çetinkaya BÖ, Şimşek SB, Köprülu D, Kahraman H. The role of periodontal disease on acute phase proteins in patients with coronary heart disease and diabetes. Turk J Med Sci 2007; 37: 39–44.
  5. Borges I, Moreira EAM, Filho WD, de Oliveira TB, da Silva MBS, Fröde AS. Proinflammatory and oxidative stress markers in patients with periodontal disease. Mediat Inflamm 2007; 2007: 45794 Liles WC. Apoptosis - Role in infection and inflammation. Curr Opin Infect Dis 1997; 10: 165–70.
  6. Zamzami N, Marchetti P, Castedo M, Decaudin D, Macho A, Hirsch T et al. Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell-death. J Exp Med 1995; 182: 367–77.
  7. Zamzami N, Susin SA, Marchetti P, Hirsch T, GómezMonterrey I, Castedo M et al. Mitochondrial control of nuclear apoptosis. J Exp Med 1996; 183: 1533–44.
  8. Lucas H, Bartold P, Dharmapatni A, Holding CA, Haynes DR. Inhibition of apoptosis in periodontitis. J Dental Research 2010; 89: 29–33. Voehringer DW. BCL-2 and glutathione: alterations in cellular redox state that regulate apoptosis sensitivity. Free Radical Bio Med 1999; 27: 945–50.
  9. Buttke TM, Sandstrom PA. Oxidative stress as a mediator of apoptosis. Immunology today 1994; 15: 7. Packer L, Kraemer K, Rimbach G. Molecular aspects of lipoic acid in the prevention of diabetes complications. Nutrition 2001; 17: 888–95.
  10. Packer L, Witt EH, Tritschler HJ. Alpha-lipoic acid