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Journals / Gıda / 2016 / Cilt: 41 - Sayı: 6

DETECTION OF CRONOBACTER SPP. IN POWDERED INFANT FORMULA BY PCR

BEBEK MAMALARINDA DİREKT PCR YÖNTEMİ İLE CRONOBACTER SPP. TESPİTİ

Gökçe Polat YEMİŞ
İbrahim ÇAKIR
Kadir HALKMAN
Journal
Gıda
Pages
411–418
Publication Date
2016-12-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The result showed thatthe modified PCR based technique described in this study was found more rapid and sensitive than theconventional methods for detection of Cronobacter spp. from infant formula

📌 3 Key Takeaways

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Objective & Hypothesis:Cronobacter spp. causes meningitis, sepsis, and necrotizing enterocolitis in neonates and infants.Cronobacter spp. infections have been epidemiologically associated with consumption of reconstitutedpowdered infant formula.
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Key Findings & Data:Detection limit of PCR assay for Cronobacter spp. was determined to be 102CFU/mL directly and 1 CFU/mL after 4-h of enrichment step in infant formula.
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Conclusion & Impact:The result showed thatthe modified PCR based technique described in this study was found more rapid and sensitive than theconventional methods for detection of Cronobacter spp. from infant formula
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Cronobacter spp. · bebek maması · izolasyon

Abstract

Cronobacter spp. causes meningitis, sepsis, and necrotizing enterocolitis in neonates and infants.Cronobacter spp. infections have been epidemiologically associated with consumption of reconstitutedpowdered infant formula. In this study, the modified PCR method for the rapid detection of Cronobacterspp. were performed. Detection limit of PCR assay for Cronobacter spp. was determined to be 102CFU/mL directly and 1 CFU/mL after 4-h of enrichment step in infant formula. The result showed thatthe modified PCR based technique described in this study was found more rapid and sensitive than theconventional methods for detection of Cronobacter spp. from infant formula

Özet

Cronobacter spp., bebeklerde ve çocuklarda hayati tehlike yaratan menenjit, sepsis ve nekrotizanenterokolit enfeksiyonlarının önemli bir sebebidir. Çeşitli klinik vakalarda Cronobacter spp. enfeksiyonuile bebek maması tüketimi epidemiyolojik olarak ilişkili bulunmuştur. Bu araştırmada bebek mamasındapatojenin hızlı tanısı için direkt PCR yöntemi üzerine modifikasyon çalışmaları yapılmıştır. Bebekmamasında direkt PCR yöntemi ile 102KOB/mL düzeyinde ve 4 saatlik ön zenginleştirme sonrasındauygulanan PCR yöntemi ile 1 KOB/mL seviyesinde Cronobacter spp. belirlenebilmiştir. Cronobacterspp. tespitinde uygulanan PCR yönteminin kültürel belirleme yöntemlerine oranla daha hızlı ve dahaduyarlı olduğu saptanmıştır

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Cite This Article

YEMİŞ, G. P., ÇAKIR, İ., HALKMAN, K. (2016). DETECTION OF CRONOBACTER SPP. IN POWDERED INFANT FORMULA BY PCR. Gıda, Cilt: 41 - Sayı: 6, 411–418. https://ajindex.com/articles/39348

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References (9)

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