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Journals / Gıda / 2019 / Cilt: 44 - Sayı: 3

ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES

LISTERIA MONOCYTOGENES SPESİFİK FAJLARIN İZOLASYONU

Pinar ŞANLIBABA
Başar UYMAZ TEZEL
Journal
Gıda
Pages
463–471
Publication Date
2019-05-15
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

None of the phages had identical host ranges.

📌 3 Key Takeaways

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Objective & Hypothesis:It was aimed to isolate, purify and determine host ranges of lytic phages that infect Listeria monocytogenes in this study.
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Key Findings & Data:Out of 68 samples screened, 4 positive isolates were recovered from feces, food processing waste water, fisheries waste water, and fish samples.
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Conclusion & Impact:None of the phages had identical host ranges.
🔬Design:Research Article
📍Scope:68 samples
⚙️Variables:Listeria monocytogenes · Faj · İzolasyon

Abstract

It was aimed to isolate, purify and determine host ranges of lytic phages that infect Listeria monocytogenes in this study. Out of 68 samples screened, 4 positive isolates were recovered from feces, food processing waste water, fisheries waste water, and fish samples. Recovery status of the L. monocytogenes phage was found to be 5.88%. To determine host ranges of phages, soft agar overlay plaque assay was used. While eleven L. monocytogenes strains showed resistant to all four isolated phages, 24 strains were sensitive. The plaque sizes of the 4 phages against 24 L. monocytogenes strains ranged from 0.4 and 2.6 mm. None of the phages had identical host ranges.

Özet

Bu çalışmada Listeria monocytogenes'i enfekte eden litik fajların izole edilmesi, saflaştırılması ve konakçı etkinliklerinin belirlenmesi amaçlanmıştır. Toplamda 68 örnek taranmış ve dışkı, gıda işleme atık suları, balıkçılık atık suları ve balık örneklerinden 4 pozitif izolat elde edilmiştir. Faj varlığı bakımından taranan örneklerin % 5.88’inde L. monocytogenes fajı izole edilmiştir. Faj konakçı etkinliğinin belirlenmesi amacıyla, çift tabaka agar yöntemi kullanılmıştır. 11 L. monocytogenes suşu denenen dört faja karşı dirençli bulunurken, 24 suşta ise duyarlılık saptanmıştır. Dört fajın 24 L. monocytogenes suşuna karşı oluşturduğu faj plak çapı ise 0.4 ve 2.6 mm arasında değişmiştir. Hiçbir faj birbiri ile aynı konakçı etkinliği göstermemiştir.

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Cite This Article

ŞANLIBABA, P., TEZEL, B. U. (2019). ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES. Gıda, Cilt: 44 - Sayı: 3, 463–471. https://ajindex.com/articles/39111

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References (5)

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