AI Summary & Key Takeaways
AI InsightNisin resistance levels in recombinant LL175, LL176 and LL178 strains were increased to 300 IU $mL^ {-1}$, 350 IU $mL^ {-1}$ and 500 IU mL1levels, respectively, however it was decreased to 100 IU $mL^ {-1}$ in recombinant strain LL177.
📌 3 Key Takeaways
Abstract
In this study, expression of nisin resistance (nisFEG ve nisI), regulation (nisRK) and regulation/resistance (nisRKFEG) genes in lacticin 481 producer strain Lactococcus lactis subsp. lactis MBLL9, was investigated. Lacticin 481 production levels for recombinant strains expressing nisFEG (LL175), nisI (LL176), nisRK (LL177), nisRKFEG (LL178) genes and control strain (MBLL9) were determined as 80.000 AU $L^ {-1}$, 80.000 AU $L^ {-1}$, 40.000 AU $L^ {-1}$, 40.000 AU $L^ {-1}$ and 80.000 AU $L^ {-1}$, respectively. Nisin resistance levels in recombinant LL175, LL176 and LL178 strains were increased to 300 IU $mL^ {-1}$, 350 IU $mL^ {-1}$ and 500 IU mL1levels, respectively, however it was decreased to 100 IU $mL^ {-1}$ in recombinant strain LL177.
Özet
Bu çalışmada, nisin dirençlilik (nisFEG ve nisI), regülasyon (nisRK) ve regülasyon/dirençlilik (nisRKFEG) genlerinin laktisin 481 üreticisi Lactococcus lactis subsp. lactis MBLL9 suşundaki ifadesi araştırıldı. nisFEG (LL175), nisI (LL176), nisRK (LL177), nisRKFEG (LL178) genlerini ifade eden rekombinant suşların ve kontrol suşun (MBLL9) laktisin 481 üretiminin, sırasıyla 80.000 AU $L^ {-1}$, 80.000 AU $L^ {-1}$, 40.000 AU $L^ {-1}$, 40.000 AU $L^ {-1}$ ve 80.000 AU $L^ {-1}$ düzeylerinde olduğu tespit edildi. Rekombinant suşlarda nisin dirençliliğin, LL175, LL176 ve LL178 suşlarında sırasıyla 300 IU $mL^ {-1}$, 350 IU $mL^ {-1}$ ve 500 IU $mL^ {-1}$ düzeylerine yükseldiği, LL177 suşunda ise 100 IU $mL^ {-1}$ düzeyine düştüğü saptandı.