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Journals / Experimed / 2021 / Cilt: 11 - Sayı: 2

The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos

Sodyum Lauril Sülfatın Gelişmekte Olan Zebra Balığı Embriyolarında Biyouyumluluğu

Simge MEŞELİ
Gül KAPLAN
Derya CANSIZ
Ünsal Veli ÜSTÜNDAĞ
İsmail ÜNAL
Ebru EMEKLİ ALTURFAN
Funda YANIKOĞLU
Dilek TAĞTEKİN
Journal
Experimed
Pages
67–72
Publication Date
2021-08-25
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: It may be suggested that LPO is an early indicator of exposure to SLS during embryogenesis and further studies are re-quired to confirm this finding.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: As an anionic surfactant, sodium lauryl sulphate (SLS), is used as an emulsifying agent in toothpastes as well as many dif-ferent household cleaning products.
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Key Findings & Data:Material and Method: Zebrafish embryos that were dividing nor-mally and which had spherical shapes were chosen and they were ex-posed to SLS both in low and high concentrations in well plates for 72 hours.
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Conclusion & Impact:Conclusion: It may be suggested that LPO is an early indicator of exposure to SLS during embryogenesis and further studies are re-quired to confirm this finding.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Sodyum lauril sülfat · diş macunu · lipit peroksi-dasyon

Abstract

Objective: As an anionic surfactant, sodium lauryl sulphate (SLS), is used as an emulsifying agent in toothpastes as well as many dif-ferent household cleaning products. Many toothpastes contain SLS and it is responsible for the formation of foam when brush-ing. However SLS may also irritate sensitive teeth and gums. Lip-id peroxidation (LPO) is defined as an autoxidation process that is induced through the attack of free oxygen radicals leading to oxidative stress in the pathophysiology of various diseases. LPO causes the formation of highly reactive products including differ-ent aldehydes, ketones, and alkanes serving as biomarkers of LPO. In our study we aimed to expose zebrafish embryos to SLS and de-termine LPO in SLS exposed zebrafish embryos. Material and Method: Zebrafish embryos that were dividing nor-mally and which had spherical shapes were chosen and they were ex-posed to SLS both in low and high concentrations in well plates for 72 hours. Rates of mortality and hatching were determined. The levels of malondialdehyde were evaluated using the Yagi's method as the end products of LPO in the form of thiobarbituric acid reactive substances. Results: Our findings showed LPO increased significantly in both low SLS (p<0.05) and high dose SLS (p<0.05) exposed zebrafish embryos when they were compared to the control group. Conclusion: It may be suggested that LPO is an early indicator of exposure to SLS during embryogenesis and further studies are re-quired to confirm this finding.

Özet

Amaç: Sodyum lauril sülfat (SLS), ev temizlik ürünleri ve diş macun-larında emülsifiye edici temizlik maddesi olarak kullanılan anyonik bir yüzey aktif maddedir. Birçok diş macunu fırçalarken köpük oluşumuna yol açan SLS içerir. Ancak SLS hassas diş ve diş etlerini tahriş edebilir. Lipid peroksidasyonu (LPO), çeşitli hastalıkların patofizyo-lojisinde oksidatif strese yol açan serbest radikallerin saldırısı ile başlatılan bir otooksidasyon sürecidir. LPO, LPO'nun biyobelirteçleri olarak görev yapan farklı aldehitler, ketonlar, alkanlar dahil olmak üzere reaktif ürünlerin oluşumuna neden olur. Çalışmamızın amacı, SLS'ye maruz kalan zebra balığı embriyolarında oluşan LPO'yu değerlendirmektir.Gereç ve Yöntem: Normal olarak bölünen küresel embriyolar, 72 saat boyunca plaka kuyucuklarında düşük ve yüksek dozda SLS'ye maruz bırakılmıştır. Mortalite ve kuluçkadan çıkma oranları belir-lenmiştir. Tiyobarbitürik asit reaktif maddeler olarak LPO'nun son ürünü olan malondialdehit (MDA) düzeyini belirlemek için Yagi yöntemi kullanılmıştır.Bulgular: Bulgularımız, hem düşük SLS (p<0,05) hem de yüksek doz SLS’ye (p<0,05) maruz kalan zebra balığı embriyolarında kont-rol grubu ile karşılaştırıldığında LPO'nun önemli ölçüde arttığını göstermiştir.Sonuç: LPO'nun embriyogenez sırasında SLS'ye maruz kalmanın erken bir göstergesi olduğu öne sürülebilir ve bu bulguyu doğru-lamak için farklı çalışmaların yapılması gerektiği düşünülmektedir.

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Cite This Article

MEŞELİ, S., KAPLAN, G., CANSIZ, D., ÜSTÜNDAĞ, Ü. V., ÜNAL, İ., ALTURFAN, E. E., YANIKOĞLU, F., TAĞTEKİN, D. (2021). The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos. Experimed, Cilt: 11 - Sayı: 2, 67–72. https://ajindex.com/articles/386290

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References (10)

  1. Magny R, Auzeil N, Olivier E, Kessal K, Regazzetti A, Dutot M, et al. Lipidomic analysis of human corneal epithelial cells exposed to ocular irritants highlights the role of phospholipid and sphin-golipid metabolisms in detergent toxicity mechanisms. Biochimie 2020
  2. [CrossRef] google scholar 2. Bondi CAM, Marks JL, Wroblewski LB, Raatikainen HS, Lenox RS, Gebhardt KE. Human and Environmental Toxicity of Sodium Lau-ryl Sulfate (SLS): Evidence for Safe Use in Household Cleaning Products. Environ Health Insights 2015
  3. [CrossRef] google scholar 3. Cosmetic Ingredient Review (CIR). Final report on the safety as-sessment of sodium lauryl sulfate and ammonium lauryl sulfate. Int J Toxicol 1983
  4. 2(7): 1-34. google scholar 4. Robinson VC, Bergfeld WF, Belsito DV, Hill RA, Klaassen CD, Marks JG, et al. Final report of the amended safety assessment of sodium lauryl sulfate and related salts of sulfated ethoxylated alcohols. Int J Toxicol 2010
  5. 29(4): 151S-61S. google scholar 5. Proctor & Gamble (P&G). Safety Data Sheets. Accessed August 19, 2015. Available from: URL: http://www.pgprod-uctsafety.com/ productsafety. google scholar 6. Seventh Generation. Material Safety Data Sheets. Accessed Au-gust 17, 2015. Available from: URL: http://www.sev- enthgenera-tion.com/material-safety-data-sheets. google scholar 7. Lippert F. An introduction to toothpaste its purpose, history and ingredients. Van Loveren C. Toothpastes. 23. Basel: Karger
  6. p. 1-14. google scholar 8. Jenkins S, Andy M, Newcombe R. Triclosan and sodium lauryl sul-phate mouth rinses effects on salivary bacterial counts. J Clin Peri-odontol 1991
  7. 18(2):140-8. [CrossRef] google scholar 9. Herlofson BB, Barkvoll P. Oral Mucosal desquamation caused by two toothpaste detergents in an experimental model. Eur J Oral Sci 1996
  8. 104(1): 21-6. google scholar 10. Material Safety Data Sheet: Sodium Chloride. Accessed August 20, 2015. Available from: URL: http://www.sciencelab.com/msds. php?msdsId. google scholar 11. Product Bulletin: Sodium Lauryl Sulfate. Stepan Company, North-field, Illinois
  9. Available from: URL: https://www.stepan.com google scholar 12. Material Safty Data Sheet: STEPANOL WA-EXTRA K. Stephan Com-pany, Northfield, Illinois
  10. Available from: URL: https://www. stepan.com google scholar 13. OECD Screening Information Data Set (SIDS). Sodium Dodecyl Sulfate. August 19, 2015. Available from: URL:http://www.chem. unep.ch/irptc/sids/OECDSIDS/151213.htm google scholar 14