SavedAdd your journal

Journals / Turkish Journal of Obstetrics and Gynecology / 2017 / Cilt: 14 - Sayı: 3

Micronuclei frequencies in lymphocytes and cervical cells of women with polycystic ovarian syndrome

Polikistik over sendromlu kadınlarda lenfositler ve servikal hücrelerde mikronükleus frekansları

Rengin KARATAYLI
AYŞE GÜL ZAMANİ
KAZIM GEZGİNÇ
Ebru TUNCEZ
Sema SOYSAL
Fikriye KARANFİL
AYNUR ACAR
M. Selman YILDIRIM
Pages
151–155
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The difference regarding micronucleus frequencies in cervical cells was statistically significant between the groups (p=0.032).Conclusion: Although study group is small, our study results support that there is an increased micronucleus frequency in cervical exfoliated cells of PCOS patients; this is a determinant of genetic hazard in the disease.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: The aim of this study was to determine micronucleus (MN) frequencies in exfoliated cervical cells and peripheral blood lymphocytes of women with polycystic ovarian syndrome (PCOS).Materials and Methods: Fifteen patients with PCOS and 11 healthy control patients were included in the study.
📊
Key Findings & Data:There was no statistically significant difference between the groups regarding micronucleus frequency in peripheral blood lymphocytes (p=0.239).
💡
Conclusion & Impact:The difference regarding micronucleus frequencies in cervical cells was statistically significant between the groups (p=0.032).Conclusion: Although study group is small, our study results support that there is an increased micronucleus frequency in cervical exfoliated cells of PCOS patients; this is a determinant of genetic hazard in the disease.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this study was to determine micronucleus (MN) frequencies in exfoliated cervical cells and peripheral blood lymphocytes of women with polycystic ovarian syndrome (PCOS).Materials and Methods: Fifteen patients with PCOS and 11 healthy control patients were included in the study. Cervical smears and peripheral blood were collected from all patients. Specimens were analyzed for MN frequencies and compared between the groups. In addition to MN, other nuclear anomalies connected with both genotoxicity and cytotoxicity were evaluated.Results: The MN frequencies in cervical smear and peripheral blood lymphocytes were compared in patients with PCOS and normal controls. There was no statistically significant difference between the groups regarding micronucleus frequency in peripheral blood lymphocytes (p=0.239). The mean MN scores in exfoliated cervical cells of patients with PCOS and normal controls were 1.19±0.57 and 0.74±0.34, respectively. The difference regarding micronucleus frequencies in cervical cells was statistically significant between the groups (p=0.032).Conclusion: Although study group is small, our study results support that there is an increased micronucleus frequency in cervical exfoliated cells of PCOS patients; this is a determinant of genetic hazard in the disease.

Özet

Amaç: Bu çalışmanın amacı, polikistik over sendromlu (PKOS) kadınların eksfoliyatif servikal hücrelerinde ve periferik kan lenfositlerinde mikronükleus (MN) frekanslarını belirlemektir.Gereç ve Yöntemler: PKOS'li 15 hasta ve 11 sağlıklı kontrol hastası çalışmaya dahil edildi. Tüm hastalardan servikal smearleri ve periferik kan toplandı. Numuneler, MN frekansları açısından analiz edildi ve gruplar arasında karşılaştırıldı. Hem MN sıklığı, hem de genotoksisite ve sitotoksite bağlı diğer nükleer anomaliler değerlendirildi. Bulgular: Servikal smear ve periferik kan lenfositlerinde MN frekansları PKOS hastaları ve normal kontrollerde karşılaştırıldı. Periferik kan lenfositlerinde MN frekansı açısından gruplar arasında istatistiksel olarak anlamlı fark yoktu (p=0,239). PKOS hastalarının eksfoliyatif servikal hücrelerindeki ortalama MN skorları ve normal kontrollerinki sırasıyla 1,19±0,57 ve 0,74±0,34 idi. Servikal hücrelerdeki MN frekansları istatistiksel olarak gruplar arasında anlamlıydı (p=0,032). Sonuç: Çalışma grubu küçük olmasına rağmen çalışma sonuçlarımız, PKOS hastalarının eksfoliyatif hücrelerinde, hastalıkta genetik tehlikenin belirleyicisi olan MN sıklığının arttığını desteklemektedir.

🔖

Cite This Article

KARATAYLI, R., ZAMANİ, A. G., GEZGİNÇ, K., TUNCEZ, E., SOYSAL, S., KARANFİL, F., ACAR, A., YILDIRIM, M. S. (2017). Micronuclei frequencies in lymphocytes and cervical cells of women with polycystic ovarian syndrome. Turkish Journal of Obstetrics and Gynecology, Cilt: 14 - Sayı: 3, 151–155. https://ajindex.com/articles/376423

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Asunción M, Calvo RM, San Millán JL, Sancho J, Avila S, EscobarMorreale HF. A prospective study of the prevalence of the polycystic ovary syndrome in unselected Caucasian women from Spain. J Clin Endocrinol Metab 2000;85:2434-8
  2. Fenech M, Holland N, Zeiger E, Chang WP, Burgaz S, Thomas P, et al. The HUMN and HUMNxL international collaboration projects on human micronucleus assays inlymphocytes and buccal cells--past, present and future. Mutagenesis 2011;26:239-45
  3. Barber TM, Franks S. Genetics of polycystic ovary syndrome. Front Horm Res 2013;40:28-39
  4. Escobar-Morreale HF, Luque-Ramírez M, San Millán JL. The molecular-genetic basis of functional hyperandrogenism and the polycystic ovary syndrome. Endocr Rev 2005;26:251-82
  5. Fenkci V, Fenkci S, Yilmazer M, Serteser M. Decreased total antioxidant status and increased oxidative stress in women with polycystic ovary syndrome may contribute to the risk of cardiovascular disease. Fertil Steril 2003;80:123-7
  6. Yesilada E, Sahin I, Ozcan H, Yildirim IH, Yologlu S, Taskapan C. Increased micronucleus frequencies in peripheral blood lymphocytes in women with polycystic ovary syndrome. Eur J Endocrinol 2006;154:563-8
  7. Carrano AV, Natarajan AT. International Commission for Protection Against Environmental Mutagens and Carcinogens. ICPEMC publication no
  8. Considerations for population monitoring using cytogenetic techniques. Mutat Res 1988;204:379-406
  9. Stich HF, Rosin MP. Quantitating the synergistic effect of smoking and alcohol consumption with the micronucleus test on human buccal mucosa cells. Int J Cancer 1983;31:305-8
  10. Slavotinek A, McMillan TJ, Steel CM. A comparison of micronucleus frequency and radiation survival in lymphoblastoid cell lines. Mutagenesis 1993;8:569-75
  11. Tomanin R, Ballarin C, Nardini B, Mastrangelo G, Sarto F. Influence of smoking habit on the frequency of micronuclei in human lymphocytes by the cytokinesis block method. Mutagenesis 1991;6:123-6
  12. Eastham AM, Atkinson J, West CM. Relationships between clonogenic cell survival, DNA damage and chromosomal radiosensitivity in nine human cervix carcinoma cell lines. Int J Radiat Biol 2001;77:295- 302
  13. Ozkal P, Ilgin-Ruhi H, Akdogan M, Elhan AH, Kaçar S, Sasmaz N. The genotoxic effects of hepatitis B virus to host DNA. Mutagenesis 2005;20:147-50
  14. Ames BN, Gold LS, Willett WC. The causes and prevention of cancer. Proc Natl Acad Sci U S A 1995;92:5258-65
  15. Simic MG. DNA markers of oxidative processes in viv