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Journals / Turkish journal of nephrology (Online) / 2021 / Cilt: 30 - Sayı: 4

Effect of Epigallocatechin Gallate on Cisplatin-Induced Nephrotoxicity in Rats

Mustafa TİMURKAN
Mustafa DEMİR
Muhammet Rıdvan GÖMLEKSİZ
İbrahim Hanifi ÖZERCAN
Kazım ŞAHİN
Ayhan DOĞUKAN
Pages
262–268
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The EGCG that reduces oxidative stress and changes the expression of Bax and Bcl-2 proteins may potentially have a significant role in the prevention of CDDP-induced renal injury.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: Nephrotoxicity caused by CDDP is attributed to an increase in kidney oxidative stress, which restricts the therapeutic application of CDDP.
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Key Findings & Data:Methods: Twenty-eight male Wistar rats (8 weeks, 200-215 g) were used in the research and were separated into 4 equal groups: (1) control rats, (2) EGCG (100 mg/kg, daily) control rats, (3) CDDP-injected rats, CDDP group (7 mg/kg, i.p., single dose), and (4) EGCG-treated (100 mg/kg, daily) plus CDDP-injected rats (EGCG + CDDP group).
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Conclusion & Impact:The EGCG that reduces oxidative stress and changes the expression of Bax and Bcl-2 proteins may potentially have a significant role in the prevention of CDDP-induced renal injury.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Özet

Objectives: Nephrotoxicity caused by CDDP is attributed to an increase in kidney oxidative stress, which restricts the therapeutic application of CDDP. EGCG is a very strong green tea-derived antioxidant. To investigate the effect of epigallocatechin gallate (EGCG) on cisplatin (CDDP)-induced lipid peroxidation and nephrotoxicity in rats. Methods: Twenty-eight male Wistar rats (8 weeks, 200-215 g) were used in the research and were separated into 4 equal groups: (1) control rats, (2) EGCG (100 mg/kg, daily) control rats, (3) CDDP-injected rats, CDDP group (7 mg/kg, i.p., single dose), and (4) EGCG-treated (100 mg/kg, daily) plus CDDP-injected rats (EGCG + CDDP group). Results: There was a substantial rise in malondialdehyde (MDA) levels in the CDDP-injected rats (P < .05). The EGCG prevented the rise of the MDA (P < .05). In CDDP-injected rats, renal Bax protein expression was substantially higher compared with control rats, and EGCG therapy significantly decreased Bax protein levels (P < .05). In EGCG + CDDPadministered rats, Bcl-2 protein expression was higher than in CDDP-injected rats (P < .05). In the EGCG + CDDP group, the expression of renal heat shock protein 60 (Hsp60) and heat shock protein 70 (HSP70) was significantly lower compared to CDDP-alone injected rats (P < .001). CDDP-induced renal histopathological changes were significantly improved with EGCG. Conclusion: CDDP produces oxidative stress and renal damage. The EGCG that reduces oxidative stress and changes the expression of Bax and Bcl-2 proteins may potentially have a significant role in the prevention of CDDP-induced renal injury.

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Cite This Article

TİMURKAN, M., DEMİR, M., GÖMLEKSİZ, M. R., ÖZERCAN, İ. H., ŞAHİN, K., DOĞUKAN, A. (2021). Effect of Epigallocatechin Gallate on Cisplatin-Induced Nephrotoxicity in Rats. Turkish journal of nephrology (Online), Cilt: 30 - Sayı: 4, 262–268. https://ajindex.com/articles/374791

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References (6)

  1. Perazella MA, Portilla D, Safar AM, Cisplatin Nephrotoxicity. In: UpToDate, Post, TW (Ed), UpToDate, Waltham, MA, 2021
  2. Cooley ME, Davis LE, Stefano M, Abraham J. Cisplatin: a clinical review, Part 1: current uses of cisplatin and administration guideliness. Cancer Nurs. 1994;17:283–293
  3. Zhang JG, Lindup WE. Role of mitochondria in cisplatin–induced oxidative damage exhibited by rat renal cortical slices. Biochem Pharmacol. 1993;45(11):2215–2222. [CrossRef] 4. Seven A, Candan G. Antioksidan savunma sistemleri. Cerrahpaşa J Med. 1996;27:41–55
  4. Stanner S, Weichselbaum E. Antioxidants. In: Caballero B, ed. Encyclopedia of Human Nutrition. 3rd ed. London UK: Elsevier; 2013:88–99. [CrossRef] 6. Katiyar SK, Mukhtar H. Tea antioxidants in cancer chemoprevention. J Cell Bioch Suppl. 1997;27:59–67
  5. Ahmad N, Mukhtar H. Cutaneous photochemoprotection by green tea: a brief review. Skin Pharmacol Appl Skin Physiol. 2001;14(2):69–76. [CrossRef] 8. Higdon JV, Frei B. Tea catechins and polyphenols: health effects, metabolism, and antioxidant functions. Crit Rev Food Sci Nutr. 2003;43(1):89–143. [CrossRef] 9. Na HK, Surh YJ. Modulation of Nrf2-mediated antioxidant and detoxifying enzyme induction by the green tea polyphenol EGCG. Food Chem Toxicol. 2008;46(4):1271–1278. [CrossRef] 10. Sriram N, Kalayarasan S, Sudhandiran G. Epigallocatechin-3-gallate augments antioxidant activities and inhibits inflammation during bleomycin-induced experimental pulmonary fibrosis through Nrf2-Keap1 signaling. Pulm Pharmacol Ther. 2009;22(3):221–236. [CrossRef] 11. Senthil Kumaran V, Arulmathi K, Srividhya R, Kalaiselvi P. Repletion of antioxidant status by EGCG and retardation of oxidative damage induced macromolecular anomalies in aged rats. Exp Gerontol. 2008;43(3):176–183. [CrossRef] 12. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680–685. [CrossRef] 13. Pawlina W, (ed). Histology: A Text and Atlas with Correlated Cell and Molecular Biology, Philedelphia. Tokyo: Wolters Kluwer Health; 2016
  6. Manohar S, Leung N. Cisplatin nephrotoxicity: A review of the literature. J Nephrol. 2018;31(1):15–25. [CrossRef] 15. Chirino YI, Pedraza-Chaverri J. Role of oxidative and nitrosative stress in cisplatin-induced nephrotoxicity. Exp Toxicol Pathol. 2009;61(3):223–242. [CrossRef] 16. Yoshida M, Iizuka K, Terada A, et al. Prevention of nephrotoxicity of cisplatin by repeated oral administration of ebselen in r