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Journals / Turkish Journal of Immunology / 2017 / Cilt: 5 - Sayı: 1

Propolis Ekstresinin CD8+ ve CD4+CD25+ T Hücrelerindeki Granzim İfadesi Üzerine Olan Bağışıklık Düzenleyici Etkileri

Immunomodulatory Effect of Propolis Extract on Granzyme Expression in CD8+ and CD4+CD25+ T Cells

Zauhani KUSNUL
Pudji RAHAYU
Muhaimin RİFAİ
Edi WİDJAJANTO
Pages
13–19
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Ekstre, CD4+ CD25+ T lenfositleri ile CD8+ T lenfositlerinde granzim ifadesini değiştirmekte, ancak ekstrenin CD4+ , CD+ CD25+ ve CD8+ T lenfositlerinde granzim ifadesine etkisi görülmemektedir.

📌 3 Key Takeaways

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Objective & Hypothesis:Amaç: Çalışmanın amacı, propolis ekstresinin bağışıklık düzenleyici bir madde olarak CD8+ ve CD4+ CD25+ düzenleyici T hücrelerindeki granzim ifadesi üzerine olan etkisini incelemektir.
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Key Findings & Data:Gereçler ve Yöntemler: Evre 3 serviks kanserli hastalardan elde edilen 10(6) hücre/mL konsantrasyonundaki Periferik Mononükleer Kan Hücreleri(PMKH) izole edilerek propolis eklenen ve eklenmeyen kaplarda kültüre edildi.
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Conclusion & Impact:Ekstre, CD4+ CD25+ T lenfositleri ile CD8+ T lenfositlerinde granzim ifadesini değiştirmekte, ancak ekstrenin CD4+ , CD+ CD25+ ve CD8+ T lenfositlerinde granzim ifadesine etkisi görülmemektedir.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Amaç: Çalışmanın amacı, propolis ekstresinin bağışıklık düzenleyici bir madde olarak CD8+ ve CD4+ CD25+ düzenleyici T hücrelerindeki granzim ifadesi üzerine olan etkisini incelemektir. Gereçler ve Yöntemler: Evre 3 serviks kanserli hastalardan elde edilen 10(6) hücre/mL konsantrasyonundaki Periferik Mononükleer Kan Hücreleri(PMKH) izole edilerek propolis eklenen ve eklenmeyen kaplarda kültüre edildi. Hücreler, %10 FBS, 10 U/ml penisilin, 100 U/mL streptomisin, 2mM L-glutamin ve 50 mikroM 2-ME içeren 24 kuyulu kültür kaplarına paylaştırıldı. Bir PMKH popülasyonu Propolis uygulanmadan kontrol grubu olarak oluşturuldu. Hücreler %5 CO2 içeren kuluçka makinesinde(inkübatör) 48 saat kültüre edildi. Granzim ifade eden CD8+, CD4+CD25+ düzenleyici T lenfositleri akan hücre ölçer ile irdelendi. Bulgular: Propolis uygulanan CD4+ , CD4+ CD25+ ve CD8+ T lenfositlerinin sayıları anlamlı ölçüde azalmadı. CD8+ Granzim+ ve CD4+ CD25+ Granzim+ hücrelerin sayıları, propolis uygulanan hastalarda farklı idi. Sonuç: Sonuç olarak, propolis ekstresi serviks kanserli hastalarda bağışıklığı değiştiren bir madde olarak etki etmektedir. Ekstre, CD4+ CD25+ T lenfositleri ile CD8+ T lenfositlerinde granzim ifadesini değiştirmekte, ancak ekstrenin CD4+ , CD+ CD25+ ve CD8+ T lenfositlerinde granzim ifadesine etkisi görülmemektedir.

Özet

Objective: The purpose of this study was to explore the effect of propolis extract on CD8+ and CD4+ CD25+ regulatory T cell populations and granzyme expression in both cell populations, as propolis has been suggested as an immunomodulatory agent. Materials and Methods: PBMC containing 1x106 cells/ml from cervical cancer stage 3 patients were isolated and cultured with and without propolis extract. The cells were plated in a 24-well plate with RPMI medium supplemented with 10% FBS, 10 U/ml penicillin, 100 U/ml streptomycin, 2 mM L-glutamine and 50 μM 2-ME. Propolis extract was added (5, 10, 25 and 50 μg/ml) to PBMC culture. Negative control was made from PBMC culture without propolis extract for comparison. The cells were incubated for 48 hours in a 5% CO2 incubator at 37ºC. The proportion of CD8+ and CD4+ CD25+ regulatory T cells that expressed granzyme were measured by flow cytometry. Results: The results obtained that the level of CD4+ , CD4+ CD25+ , and CD8+ T cells were not markedly depleted in propolis treatment in all doses compared to control group. The percentage of CD8+ Granzyme+ and CD4+ CD25+ Granzyme+ were significantly different in groups of propolis treatment compared to control group. Conclusion: In summary, propolis extract may serve as an immunomodulatory agent in cervical cancer patients. Propolis extract could modulate the expression CD4+ CD25+ T cells expressing granzyme and CD8+ T cells expressing granzyme while it has no effect on CD4+ , CD4+ CD25+ , and CD8+ T cells

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Cite This Article

KUSNUL, Z., RAHAYU, P., RİFAİ, M., WİDJAJANTO, E. (2017). Propolis Ekstresinin CD8+ ve CD4+CD25+ T Hücrelerindeki Granzim İfadesi Üzerine Olan Bağışıklık Düzenleyici Etkileri. Turkish Journal of Immunology, Cilt: 5 - Sayı: 1, 13–19. https://ajindex.com/articles/374512

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