Journals / Toraks Dergisi / 2005 / Cilt: 6 - Sayı: 1

The diagnostic yield of bedside inoculation of pleural fluid into BACTEC liquid media in tuberculous pleurisy

Tüberküloza bağlı plevra sıvılarının yatakbaşı BACTEC sıvı besiyerine inokülasyonunun tanısal verimliliği

Pages
37–43
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

In conclusion, bedside inoculation of PF into the BACTEC liquid medium enhanced the isolation rate of M. tuberculosis as compared with the cultivation methods performed in the L-J medium.

📌 3 Key Takeaways

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Objective & Hypothesis:This comparative and prospective study was designed in order to investigate the diagnostic value of bedside inoculation of pleural fluids (PF), which were obtained from the adult patients with tuberculous pleurisy, into the BACTEC liquid medium.
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Key Findings & Data:PF were subdivided into three groups: Group 1 was inoculated into the liquid medium at the bedside.
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Conclusion & Impact:In conclusion, bedside inoculation of PF into the BACTEC liquid medium enhanced the isolation rate of M. tuberculosis as compared with the cultivation methods performed in the L-J medium.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Tüberküloz · plöral · Erişkin

Abstract

This comparative and prospective study was designed in order to investigate the diagnostic value of bedside inoculation of pleural fluids (PF), which were obtained from the adult patients with tuberculous pleurisy, into the BACTEC liquid medium. Thoracentesis and pleural biopsy were performed for all the patients with exudative pleurisy. PF were subdivided into three groups: Group 1 was inoculated into the liquid medium at the bedside. Group 2 and 3 were inoculated into the Lowenstein-Jensen (L-J) medium after being transported to the laboratory in the tubes with and without anticoagulant, respectively. Additionally, cytological analysis was done for all PF. Histopathological analysis and cultivation in the L-J medium were performed for all biopsy materials. Of the 66 cases, a total of 43 were diagnosed as tuberculosis. M. tuberculosis was isolated in 16 (37.2%) of the PF inoculated at the bedside. Mycobacterial growth in the pleural biopsies cultivated in the L-J medium was observed the bedside. None of the PF transported in the tubes with or without anticoagulant and cultivated in the L-J medium showed mycobacterial growth. Resistance against H was found in one of the cases with positive mycobacterial culture, whereas another case was resistant to both H and S. In conclusion, bedside inoculation of PF into the BACTEC liquid medium enhanced the isolation rate of M. tuberculosis as compared with the cultivation methods performed in the L-J medium.

Özet

Bu çalışma, tüberküloz plörezili erişkin hastalarda plevra sıvısının yatakbaşı (BACTEC) sıvı besiyerine inokülasyonunun tanısal değerini araştırmak amacıyla yapılmış karşılaştırmalı ve prospektif bir çalışmadır. Çalışmaya alınan tüm eksüdatif plörezili olgulara plevral ponksiyon ve plevral biyopsi yapıldı. Plevral sıvı örnekleri 3 gruba ayrıldı: Grup 1 yatakbaşı sıvı besiyerine inoküle edildi. Grup 2 ve 3 sırasıyla antikoagülanlı ve antikoagülansız tüpler içinde laboratuvara gönderilerek Löwenstein-Jensen (L-J) katı besiyerine inoküle edildi. Ayrıca plevral sıvıların tümünün sitolojik incelemesi yapıldı. Plevral biyopsi örneklerinin histopatolojik incelemesi ve L-J besiyerinde kültürü yapıldı. Altmış altı olgudan 43’üne tüberküloz tanısı konuldu. Bunların 24’üne (%55.81) yalnız histopatolojiyle, 15’ine (%34.88) hem histopatoloji hem de bakteriyolojiyle, 2’sine (%4.65) yalnız bakteriyolojiyle ve 2’sine (%4.65) de klinik ve radyolojik olarak tanı konulmuştur. Yatakbaşı sıvı besiyerine inoküle edilen plevral sıvıların 16’sında (%37.2) M. tuberculosis izole edilmiştir. L-J besiyerine ekilen plevral biyopsi örneklerinin 4’ünde (%9.3) üreme olmuş ve bunların 3’ünün aynı zamanda yatakbaşı sıvı besiyerine inoküle edilen plevra sıvılarında da üreme saptanmıştır. Antikoagülanlı ve antikoagülansız kaplarla taşınan ve L-J besiyerine ekilen plevral sıvıların hiçbirinde üreme saptanmamıştır. Üreme saptanan olguların birinde H direnci ve başka birinde de H+S direnci saptanmıştır. Sonuç olarak TB plörezi düşünülen olgularda plevra sıvısının yatakbaşı BACTEC sıvı besiyerine inokülasyonunun (L-J besiyerine göre) M. tuberculosis’in üreme oranını artırdığı saptanmıştır.

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Cite This Article

YALÇIN, Y. A., ERER, O. F., BİÇMEN, C., ÖZKAN, S. A. (2005). The diagnostic yield of bedside inoculation of pleural fluid into BACTEC liquid media in tuberculous pleurisy. Toraks Dergisi, Cilt: 6 - Sayı: 1, 37–43. https://ajindex.com/articles/368234

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