Journals / Tekirdağ Ziraat Fakültesi Dergisi / 2015 / Cilt: 12 - Sayı: 1

Kırklareli nde Üretilen Bazı Süt Ürünlerinin Mikrobiyolojik Kalitesinin Araştırılması: Salmonellaspp. ve Listeria Monoctyogenes in Real Time PCR Kullanarak Teşhis Edilmesi

Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR

Pages
74–80
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results also showed poor microbiological quality and inadequateproduction conditions of these products.

📌 3 Key Takeaways

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Objective & Hypothesis:This study was carried out for the evaluation of the microbiological quality of various dairy productsincluding white cheese, kashar cheese, butter, and milk cream purchased in retail markets in the province Kirklareli.
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Key Findings & Data:Coliform bacteria, Escherichia coliand mold-yeast counts were investigated by the standard cultural method and found to be 59 (74.7 %) and 54 (68.4 %), and 68 (86.1 %) of the total 79 samples, respectively.
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Conclusion & Impact:These results also showed poor microbiological quality and inadequateproduction conditions of these products.
🔬Design:Research Article
📍Scope:79 samples
⚙️Variables:Domain Parameters

Abstract

This study was carried out for the evaluation of the microbiological quality of various dairy productsincluding white cheese, kashar cheese, butter, and milk cream purchased in retail markets in the province Kirklareli. Coliform bacteria, Escherichia coliand mold-yeast counts were investigated by the standard cultural method and found to be 59 (74.7 %) and 54 (68.4 %), and 68 (86.1 %) of the total 79 samples, respectively. Staphylococcusaureuswas detected in 18 (22.8 %) samples. Listeria monocytogenesand Salmonella spp detected by the Real Time PCR and Listeria monocytogeneswas detected in only two kashar cheese samples, while Salmonella spp. were not detected in any samples . The microbiological contamination level was higher than the permitted level according to the European standard in most of the investigated products in spite of the low level of the pathogen detection. These results also showed poor microbiological quality and inadequateproduction conditions of these products.

Özet

Bu çalışma Kırklareli piyasasından temin edilen beyaz peynir, kaşar peynir, tereyağı, ve krema gibi çeşitli süt ürünlerinin mikrobiyolojik kalitelerinin belirlenmesi amacıyla yürütülmüştür. 79 örnekten 59 (%74,7), 54 (%68,4) ve 68 (%86,1) örnekte sırasıyla Koliformbakteri, E.colive küf/maya bulunmuştur. Staphylococcusaureus ise18 (% 22, 8) örnekte tespit edilmiştir. Listeria monoctyogenesyalnızca 1 kaşar örneğinde tespit edilirken, herhangi bir örnekte Salmonellaspp. bulgusuna rastlanılmamıştır. Yalnızca bir örnekte patojen mikroorganizma bulgusuna rastlanmasına rağmen örneklerin çoğunun mikrobiyolojik yükü Avrupa standartlarına göre izin verilen seviyenin üzerinde çıkmıştır. Bu sonuçlar ürünlerin mikrobiyolojik kalitesinin çok düşük olduğuna ve yetersiz hijyen ve sanitasyona işaret etmektedir.

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Cite This Article

ÇETİN, B., KARASU, S., ATİK, A., DURAK, M. Z. (2015). Kırklareli nde Üretilen Bazı Süt Ürünlerinin Mikrobiyolojik Kalitesinin Araştırılması: Salmonellaspp. ve Listeria Monoctyogenes in Real Time PCR Kullanarak Teşhis Edilmesi. Tekirdağ Ziraat Fakültesi Dergisi, Cilt: 12 - Sayı: 1, 74–80. https://ajindex.com/articles/367094

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References (10)

  1. Akıneden Ö, Hassan AA, Schneider E and Usleber E (2008) Enterotoxigenic Properties of Staphylococcus AureusIsolated from Goats' Milk Cheese. Int J Food Microbiol. 124, 211-216.
  2. Aksu H, Çolak H, Vural A and Erkan ME (1999) Diyarbakır Bölgesinde Üretilen Örgü Peynirlerde Mikrobiyolojik ve Kimyasal Özellikler Üzerine Bir Araştırma. YYU Vet Fak Derg. 10, 8-11.
  3. Akyüz N, Tutşı MF, Mengel Z, Ocak E and Altun I (1998)
  4. Örgü Peynirinin Üretim Tekniği, Bazı Mikrobiyolojik Ve Kimyasal Özellikleri. V. Süt Ve Süt Ürünleri Sempozyumu-Geleneksel Süt Ürünleri. Milli Prodüktivite Merkezi Yayınları, Pp 328-337, Ankara. Aman IM,Knappsteın K and Hahn G (1998) Examination Of Verotoxin Producing Escherichia Coli in Some Egyptian Dairy Products with Special Reference to Serotype O157:H7. Milchwissenschaft, 53: 676–679. Andre MCDPB, Campos MRH, Borges LJ, Kıpnıs A, Pımenta FC and Serafini AB (2008) Comparison of Staphylococcus Aureus İsolates from Food Handlers, Raw Bovine Milk and Minas Frescal Cheese By Antibiogram and Pulsed-Field Gel Electrophoresis Following Smaı Digestion. Food Control 19, 200–207. Anonymous (2011).
  5. Mikrobiyolojik Kriterler Tebliği, Türk Gıda Kodeksi Yönetmeliği, 29.12. 2011 tarihli 28157 sayılı Resmi Gazete, Ankara.
  6. Anonymous (2013) Türkiye İstatistik Kurumu (Tüik), Temel İstatistikler, Tarım, Hayvansal Üretim, Http://www.Tuik.Gov.Tr/Ustmenu.Do?Metod=Temelist Ansay SE and Kaspar CW (1997)
  7. Survey of Retail Cheeses, Dairy Processing Environments and Raw Milk for Escherichia Coli O157:H7. Lett Appl Microbiol 25, 131–134. Arau ́Jo VS, Paglıares VA, Queıroz ML And Freitas-Almeıda AC (2002) Occurrence Of Staphylococcus and Enteropathogens in Soft Cheese Commercialized in The City Of Rio De Janeiro. Braz J Appl Microbiol 92, 1172–1177. Arici M, Demirci M and Gündüz HH (1999) An Investigation on Listeria Spp. Contamination in White Cheese Made From Sheep’s Milk in Tekirdağ. Milchwissenschaft 54, 90-91.
  8. Aygün O, Pehlivanlar S (2006) Listeria Spp. in The Raw Milk and Dairy Products in Antakya, Turkey. Food Control 17, 676-679.
  9. Berrada H, Soriano JM, Picó Y and Mañes J (2008) Application of Real-Time Polymerase Chain Reaction for Rapid Determination of Salmonella In Restaurant Foods. J Rapid Meth and Auto Microbiol. 16, 299-307.
  10. Beuchat LR and Ryu J H (1997) Produce Handling and Processing Practices. Emerg Infect Dis. 3, 459–465. Can HY and Çelık TH (2012): Detection of Enterotoxigenic and Antimicrobial Resistant S. Aureus in Turkish Cheeses. Food Control 24, 100-103.