SavedAdd your journal

Journals / Şişli Etfal Hastanesi Tıp Bülteni / 2009 / Cilt: 43 - Sayı: 2

PTEN expression and its relationship with prognostic factors in gastric adenocarcinomas

Mide adenokarsinomlarında PTEN ekspresyonu ve prognostik parametrelerle ilişkisi

Cumhur Selçuk TOPAL
Müberra Seğmen YILMAZ
Murat Hakan KARABULUT
Dilek YAVUZER
Nimet KARADAYI
Pages
77–81
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result PTEN expression was found to be related to malignant transformation, tumor progression and histologic grade.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: PTEN (phosphatase and tensin) is an important molecular marker that works as a tumor supressor gene located on the 10th chromosome (10q23.3).
📊
Key Findings & Data:Study design: Slides obtained from paraffine blocks of 60 gastric adenocarcinoma immunohistochemically stained with PTEN (Clone 28H6, Novocastra, UK).
💡
Conclusion & Impact:As a result PTEN expression was found to be related to malignant transformation, tumor progression and histologic grade.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:PTEN fosfohisrolaz · Adenokarsinom · İmmünohistokimya

Abstract

Objective: PTEN (phosphatase and tensin) is an important molecular marker that works as a tumor supressor gene located on the 10th chromosome (10q23.3). In this retrospective study, PTEN expression and its relationship with prognostic parameters have been investigated. Study design: Slides obtained from paraffine blocks of 60 gastric adenocarcinoma immunohistochemically stained with PTEN (Clone 28H6, Novocastra, UK). Results: Statistically significant relationship was found between Lauren‘s classification, histologic grade and PTEN expression. But we were unable to show significant relationship with PTEN and vascular invasion, perineural invasion, lymph node metastasis, TNM (primary tumor, lymph node, distant metastasis) stage. Conclusions : We found that PTEN expression loss is more prominent in the diffuse type carcinomas than the intestinal types and also there is a reverse correlation between histologic differentiation and PTEN loss. As a result PTEN expression was found to be related to malignant transformation, tumor progression and histologic grade.

Özet

Özet: Amaç: PTEN (phosphatase and tensin); 10.kromozomda (10q23.3) lokalize tümör supresör gen olarak belirlenmiş önemli bir moleküler marker’dır. Bu retrospektif çalışmada mide adenokarsinomlarında PTEN ekspresyonu, PTEN ekspresyonunun prognostik parametre olarak anlamlılığı ve diğer prognostik parametrelerle ilişkisi araştırılmıştır. Gereç ve Yöntem: Mide adenokarsinomu tanısı almış 60 olguya ait tümör içeren parafin bloklardan elde edilen kesitlere PTEN (Clone 28H6, Novocastra, UK) ile immunohistokimyasal boyama prosedürü uygulandı. Bulgular: PTEN ekspresyonu ile Lauren sınıflaması ve histolojik grade arasında istatistiksel olarak anlamlı bir ilişki saptandı. Ancak PTEN ekspresyonu ile vasküler invazyon, perinöral invazyon, lenf nodu metastazı, TNM (primer tümör, lenf nodu, uzak metastaz) evresi arasında istatistiksel olarak anlamlı bir ilişki bulunmamıştır. Sonuç: Çalışmamızda Lauren sınıflamasına göre diffuz tip karsinomlarda, intestinal tip karsinomlara göre PTEN ekspresyon kaybının daha belirgin olduğu ve differansiasyon azaldıkça PTEN ekspresyon kaybının arttğı saptanmıştır. Sonuçta PTEN ekspresyonunun malign transformasyonla, progresyonla ve diğer prognostik parametrelerden histolojik grade ile ilişkili olduğu görülmüştür.

🔖

Cite This Article

TOPAL, C. S., YILMAZ, M. S., KARABULUT, M. H., YAVUZER, D., KARADAYI, N. (2009). PTEN expression and its relationship with prognostic factors in gastric adenocarcinomas. Şişli Etfal Hastanesi Tıp Bülteni, Cilt: 43 - Sayı: 2, 77–81. https://ajindex.com/articles/363537

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (1)

  1. Zhou XP, Gimm O, Hampel H, et al: Epigenetic PTEN silencing in malignant melanomas without PTEN mutation. Am J Pathol. 2000; 157: 1123-1128 2) Roh MS: Loss of PTEN expresion in primary lung cancer. The Korean J Pathol. 2002; 36: 286-291 3) Li J, Yen C, Liaw D, et al: PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science. 1997; 28; 275 (5308): 1943-1947. 4) Kimura F, Watanabe J, Hata H, et al: PTEN immunohistochemical expression is suppressed in G1 endometrioid adenocarcinoma of the uterine corpus. J Cancer Res Clin Oncol. 2003; 20 5) Chung MJ, Jung SH, Lee BJ: Inactivation of the PTEN gene protein product is associated with the invasiveness and metastasis, but not angiogenesis, of breast cancer. Pathol Int 2004; 54: 10-15. 6) Sano T, Lin H, Chen X, et al: Differential expression of MMAC/PTEN in glioblastoma multiforme: relationship to localization and prognosis. Cancer Res. 1999; 15; 59 (8): 1820-1824. 7) Perren A, Weng LP, Boag AH, et al: Immunohistochemical evidence of loss of PTEN expression in primary ductal adenocarcinomas of the breast. Am J Pathol. 1999; 155 (4): 1253-1260. 8) McMenamin ME, Soung P, Perara S, et al: Loss of PTEN expression in paraffine-embedded primary prostate cancer correlates with high Gleason score and advanced stage. Cancer Res 1999; 59: 4291-4296. 9) Kondo K, Yao M, Kobayashi K, et al: PTEN / MMAC1 / TEP1 mutations in human primary renal cell carcinoma and renal cell carcinoma cell lines. Int J Cancer 2001 15; 91 (2): 219-224. 10) Aveyard JS, Skilleter A, Habuchi T, Knowles MA: Somatic mutation of PTEN in bladder carcinoma. Br J Cancer. 1999; 80 (5-6): 904-908. 11) Çelebi JT, Ping XL, Zhang H, et al: Germline PTEN mutations in three families with Cowden syndrome. Exper Dermatol. 2000; 9: 152-156. 12) Marsh DJ, Dahia PL, Zheng Z, et al: Germline mutations in PTEN are present in Bannayan Zonana Syndrome. Nat Genet 1997 16 (4): 333-334. 13) Yang L, Kuang LG, Zheng HC, et al: PTEN encoding product: a marker for tumorogenesis and progression of gastric carcinoma. World J Gastrenterol 2003; 9 (1): 35-39. 14) Zheng HC, Li YL, Sun JM, et al: Growth, invasion, metastasis, differantiation, angiogenesis and apoptosis of gastric cancer regulated by expression of PTEN encoding products. World J Gastroenterol 2003; 9 (8): 1662-1666. 15) Zheng HC, Sun JM, Li XF, et al: Role of PTEN and MMP-7 expression in growth, invasion, metastasis and angiogenesis of gastric carcinoma. Pathol In