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Journals / Şişli Etfal Hastanesi Tıp Bülteni / 2010 / Cilt: 44 - Sayı: 4

Fas and fas-ligand expressions and their relationship with clinicopathologic parameters in laryngeal squamous cell carcinomas

Larinks skuamöz hücreli karsinomlarında fas, fas ligand ekspresyonu ve klinikopatolojik parametreler ile ilişkisi

Murat Hakan KARABULUT
Müberra YILMAZ SEĞMEN
Cumhur Selçuk TOPAL
Medine MURTAZAOĞLU
Dilek YAVUZER
Nimet KARADAYI
Gözde KIR
Pages
145–151
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Key words: Fas, FasL, laryngeal squamous cell carcinomas

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Fas (Apo-1, CD95) and its ligand Fas-Ligand (FasL) are important molecular markers for the regulation of the apoptosis (programmed cell death).
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Key Findings & Data:In this retrospective study, expression of Fas and FasL and their relationship with the clinical, histopathologic parameters and prognosis, in squamous cell carcinomas (SCC) which consists 96% of laryngeal cancers, was investigated.
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Conclusion & Impact:Key words: Fas, FasL, laryngeal squamous cell carcinomas
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Fas (Apo-1, CD95) and its ligand Fas-Ligand (FasL) are important molecular markers for the regulation of the apoptosis (programmed cell death). In this retrospective study, expression of Fas and FasL and their relationship with the clinical, histopathologic parameters and prognosis, in squamous cell carcinomas (SCC) which consists 96% of laryngeal cancers, was investigated. Materials and method: Immunohistochemical staining for the CD95 (FAS) (Ab-4 Rabbit Polyclonal Antibody, Lab Vision Corp. Neomarkers, Fremont CA, USA. 60 minutes) and FasL (Rabbit Polyclonal Antibody, Lab Vision Corp. Neomarkers, Fremont CA, USA. 60 minutes) was applied on the slides obtained from paraffin blocks of the 53 laryngeal SCC. Results: We found significantly positive relationship (p<0.01) between the Fas expression and histologic differentiation and between Fas and FasL (p<0.05) at the tumor cells. Conclusion: In this study, Fas and FasL are found to be in correlation with histologic grade. Key words: Fas, FasL, laryngeal squamous cell carcinomas

Özet

Larinks skuamöz hücreli karsinomlarında fas, fas ligand ekspresyonu ve klinikopatolojik parametreler ile ilişkisi Amaç: Fas (CD95-Apo1) ve bunun ligandı Fas –ligand (FasL) apoptozun (programlı hücre ölümü) düzenlenmesinde önemli bir moleküler marker’dır. Bu retrospektif çalışmada larinks kanserlerinin %96’sını oluşturan skuamöz hücreli karsinomlarda (SHK) Fas ve Fas-ligand ekspresyonu ve bunun klinik , histopatolojik parametreler ile ilişkisi, prognoz üzerindeki etkileri araştırılmıştır. Materyal ve Metod: Larinkste SHK tanısı almış 53 olgunun parafin bloklarından elde edilen kesitlere CD95 (FAS) Ab-4 (Rabbit Polyclonal Antibody, Lab Vision Corp. Neomarkers, Fremont CA, USA. 60 dakika) ve FAS Ligand (Rabbit Polyclonal Antibody, Lab Vision Corp. Neomarkers, Fremont CA, USA. 60 dakika) ile immünohistokimyasal boyama prosedürü uygulandı. Bulgular: Tümör hücrelerinde Fas ekspresyonu ile; histolojik grade arasında (p<0.01) ve FasL arasında (p<0.05) pozitif yönde anlamlı bir korelasyon saptandı. Sonuç: Bu çalışmada Fas ekspresyonunun larinks SHK’larında histolojik grade ile ilişkili olduğu görülmüştür.

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Cite This Article

KARABULUT, M. H., SEĞMEN, M. Y., TOPAL, C. S., MURTAZAOĞLU, M., YAVUZER, D., KARADAYI, N., KIR, G. (2010). Fas and fas-ligand expressions and their relationship with clinicopathologic parameters in laryngeal squamous cell carcinomas. Şişli Etfal Hastanesi Tıp Bülteni, Cilt: 44 - Sayı: 4, 145–151. https://ajindex.com/articles/363514

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