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Journals / Şişli Etfal Hastanesi Tıp Bülteni / 2020 / Cilt: 54 - Sayı: 3

Identification of the Mycobacterial Strains Isolated From Clinical Specimens Using hsp65 PCR-RFLP Method

Ayse BARİS
Banu BAYRAKTAR
Pages
364–370
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In the identification of non-tuberculosis mycobacteria with the hsp65 PCR-RFLP method, one isolate wasidentified as M. abcessus and three isolates were identified as M. avium complex.Conclusion: In our study, it was concluded that the hsp65 PCR-RFLP method, which allows identification of mycobacteria, includingNTMs, is a method that is cheap, easy and suitable for routine use to provide rapid information to the clinic.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: It is important to identify mycobacteria at the species level, to distinguish pathogen from non-pathogenic species, tochoose the appropriate treatment regimen and to collect epidemiological data.
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Key Findings & Data:This study aimsto investigate the suitability of the hsp65 Polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) method forroutine laboratory use.Methods: In this study, 141 mycobacterial isolates were obtained from 1632 samples, which were sent to the Medical MicrobiologyLaboratory.Results: In the culture, mycobacteria were identified as 138 M. tuberculosis complex (MTBC) and three non-tuberculosis mycobacteria(NTM) by conventional methods.
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Conclusion & Impact:In the identification of non-tuberculosis mycobacteria with the hsp65 PCR-RFLP method, one isolate wasidentified as M. abcessus and three isolates were identified as M. avium complex.Conclusion: In our study, it was concluded that the hsp65 PCR-RFLP method, which allows identification of mycobacteria, includingNTMs, is a method that is cheap, easy and suitable for routine use to provide rapid information to the clinic.
🔬Design:Research Article
📍Scope:1632 samples
⚙️Variables:Domain Parameters

Özet

Objectives: It is important to identify mycobacteria at the species level, to distinguish pathogen from non-pathogenic species, tochoose the appropriate treatment regimen and to collect epidemiological data. For the identification of mycobacteria, which aretime-consuming and laborious with traditional methods, faster, more sensitive and reliable methods are needed. This study aimsto investigate the suitability of the hsp65 Polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) method forroutine laboratory use.Methods: In this study, 141 mycobacterial isolates were obtained from 1632 samples, which were sent to the Medical MicrobiologyLaboratory.Results: In the culture, mycobacteria were identified as 138 M. tuberculosis complex (MTBC) and three non-tuberculosis mycobacteria(NTM) by conventional methods. Using the hsp65 PCR-RFLP method, 137 isolates were identified as MTBC, four isolatesas NTM. An isolate that was evaluated as MTBC because it was PNB sensitive by the conventional method was determined as NTMwith the hsp65 method. In the identification of non-tuberculosis mycobacteria with the hsp65 PCR-RFLP method, one isolate wasidentified as M. abcessus and three isolates were identified as M. avium complex.Conclusion: In our study, it was concluded that the hsp65 PCR-RFLP method, which allows identification of mycobacteria, includingNTMs, is a method that is cheap, easy and suitable for routine use to provide rapid information to the clinic. The scope of theagar and database used in the method is effective in the definition of the correct species.

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Cite This Article

BARİS, A., BAYRAKTAR, B. (2020). Identification of the Mycobacterial Strains Isolated From Clinical Specimens Using hsp65 PCR-RFLP Method. Şişli Etfal Hastanesi Tıp Bülteni, Cilt: 54 - Sayı: 3, 364–370. https://ajindex.com/articles/363402

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