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Journals / Dicle Tıp Dergisi / 2020 / Cilt: 47 - Sayı: 1

Evaluation Of Oxidative Stress With Changes In Inflamation Parameters During Normothermic Cardiopulmonary Bypass

Normotermik Kardiyopulmoner Bypass Sırasında Enflamasyon Parametrelerindeki Değişiklikler İle Oksidatif Stresin Değerlendirilmesi

Esin AKÇA
Mehmet Salih AYDIN
Mahmut PADAK
Mustafa GÖZ
Reşat DİKME
Ömer GÖÇ
Pages
223–231
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Serum CRP levels were not statistically significant between the groups.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of this study was to evaluate the levels of CRP, ceruloplasmin and TAS (total antioxidant level), TOS (total oxidant level), OSI (oxidative stress index) levels in blood serum taken from patients undergoing cardiopulmonary bypass (CPB).
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Key Findings & Data:Methods: A study group was formed by taking 4 gel-free strains of blood from pre-operative, pump (cardiopulmonary machine) entry, cross-clamping and after protamine administration from patients who underwent surgery with CPB for various reasons.
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Conclusion & Impact:Serum CRP levels were not statistically significant between the groups.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this study was to evaluate the levels of CRP, ceruloplasmin and TAS (total antioxidant level), TOS (total oxidant level), OSI (oxidative stress index) levels in blood serum taken from patients undergoing cardiopulmonary bypass (CPB). Methods: A study group was formed by taking 4 gel-free strains of blood from pre-operative, pump (cardiopulmonary machine) entry, cross-clamping and after protamine administration from patients who underwent surgery with CPB for various reasons. Plasma samples were stored at -80 [deg.] C after removal of the blood from the centrifuge. The samples were dissolved in the autoanalyzer on the study day and serum Seruloplasmin, C-Reactive Protein (CRP), TAS, TOS and OSI were measured by colorimetric method. Results: TAS value; 0.95 ± 0.19 preoperatively, 0.73 ± 0.13 at the pump, 0.84 ± 0.18 after cross clamping, 0.92 ± 0.14 after the protamine and p <0.001, TOS value respectively; 12.66 ± 3.21, 10.22 ± 3.28, 19.04 ± 6.00, 25.88 ± 8.14 and p <0.001, OSI; 1.35 ± 0.35, 1.48 ± 0.74, 2.38 ± 0.91, 2.81 ± 0.80 and p <0.001, Seruloplasmin; 1002, 48 ± 209, 20, 554.71 ± 125.77, 581.92 ± 156.67, 633.79 ± 166.69 and p <0.001, CRP; 0.85 ± 1.44, 0.51 ± 1.09, 0.35 ± 0.44, 0.47 ± 0.89 and p = 0.291 respectively. Conclusions: As a result of this study, statistically significant differences were found between TAS, TOS, OSI and ceruloplasmin levels in the groups (p <0.001). Serum CRP levels were not statistically significant between the groups.

Özet

Amaç: Bu çalışmanın amacı, kardiyopulmoner bypass (KPB) ameliyatına giren hastalardan belirli aralıklarla alınan kanınserumundaki CRP, seruloplazmin ve TAS (total antioksidan seviye), TOS (total oksidan seviye), OSİ (oksidatif stres indeksi) seviyeleriniölçerek bir değerlendirmenin yapılması amaçlanmıştır.Yöntemler: Çeşitli sebeplerden dolayı KPB cerrahisi ile ameliyat olan hastalardan ameliyat öncesi, pompaya (kalp akciğer makinesi)giriş, kross klemp sonrası ve protamin verilmesi sonrası olmak üzere 4 jelsiz tüpe kan alınarak bir çalışma grubu oluşturulmuştur.Alınan kanlar santrifüjde ayrıştırıldıktan sonra plazmaları -80 °C’de saklanmıştır. Numuneler çalışma gününde otoanalizör cihazındaçözülerek serum Seruloplazmin, C-Reaktif Protein (CRP), TAS, TOS ve OSİ kolorimetrik yöntemle ölçülmüştür.Bulgular: TAS değeri; ameliyat öncesi 0.95 ± 0.19, pompaya giriş 0.73 ± 0.13, kross klemp sonrası 0.84 ± 0.18, protamin sonrası 0.92 ±0.14 ve p <0,001, TOS değeri sırasıyla; 12.66 ± 3.21, 10.22 ± 3.28, 19.04 ± 6.00, 25.88 ± 8.14 ve p <0,001, OSİ; 1,35 ± 0.35, 1.48±0.74,2.38 ± 0.91, 2.81 ± 0.80 ve p <0,001, Seruloplazmin; 1002,48±209,20, 554.71 ± 125.77, 581.92 ± 156.67, 633.79 ± 166.69 ve p <0,001,CRP; 0.85 ± 1.44, 0.51 ± 1.09, 0.35 ± 0.44, 0.47 ± 0.89 ve p= 0.291 olarak bulunmuştur.Sonuç: Bu çalışma sonucunda TAS, TOS, OSİ ve seruloplazmin düzeyleri gruplar arasında istatistiksel açıdan anlamlı bir farkbulunmuştur (p<0,001). Serum CRP düzeyleri gruplar arasında istatiksel açıdan anlamlı bir fark bulunamamıştır.

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Cite This Article

AKÇA, E., AYDIN, M. S., PADAK, M., GÖZ, M., DİKME, R., GÖÇ, Ö. (2020). Evaluation Of Oxidative Stress With Changes In Inflamation Parameters During Normothermic Cardiopulmonary Bypass. Dicle Tıp Dergisi, Cilt: 47 - Sayı: 1, 223–231. https://ajindex.com/articles/349890

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References (16)

  1. Giachelli CM. Vasculer Calcification Mechanisms. J Am Soc Nephrol, 2004; 15: 2959- 64
  2. Ketteler M., Bongartz P., Westenfeld R., et al. Association of low fetuin-A (AHSG) concentrations in serum with cardiovascular mortality in patients on dialysis: a crosssectional study. Lancet, 2003; 61: 827-33
  3. Merx MW., Schafer C., Westenfeld R. et al, Myocardial stiffness, cardiac remodeling, and diastolic dysfunction in calcification-prone fetuin-A-deficient mice. J Am Soc Nephrol, 2005; 16: 3357-64
  4. Lebreton JP., Joisel F., Raoult JP., et al. Serum concentration of human alpha 2 HS glycoprotein during the inflammatory process: evidence that alpha 2 HS glycoprotein is a negative acute-phase reactant. J Clin Invest, 1979; 64: 1118-29
  5. Farzaneh-Far A., Shanahan CM., Biology of Vascular Calcification in Renal Disease, Nephron Exp Nephrol, 2005; 101: 134–8
  6. Libby P., Atherom: More than mush, Lancet, 348 (supp l): 1996; s4-s7
  7. Niebauer J., Volk H-d, Kemp M., et al. Endotoxin and immune activation in chronic heart failure: a prospective cohort study. Lancet, 1999; 353: 1838–42
  8. Loudianos G., Gitlin JD. (2000), Wilson‘s Disease Sem Liv Dis, 20353-64
  9. Cross, CE., Halliwell, B., Borish, E.Tet al. Oxygen radicals and human disease, Ann. intern Med, 1987; 107: 526- 45
  10. McCord JM., Oxygen-derived free radicals in postischemic tissue injury, N. Engl. J. Med. 1985; 312: 159-63
  11. Stocker R., Yamamoto Y., McDonagh AF et al., Bilirubin is an antioxidant of possible physiological importance, Science, 1987; 235: 4792; 1043-6. 52 12. Yiğit S., Yurdakök M., Kilin K ve ark., Yenidoğanlarda serbest radikallere bağlı hastalıklar, Çocuk Sağlığı ve Hastalıkları Dergisi, 1997; 39: 749-65
  12. Moison RMW., Haasnoot AA., ZoerenGrobben DV. et al., Plasma proteins in acute and chronic lung disease of the newborn, Free Radic Biol Med, 25: 3: 321-8
  13. Erel O. (2005), A new automated colorimetric method for measuring total oxidant status, Clin Biochem, 1998; 38: 1103- 11
  14. Erel O. A novel automated direct measurement method for total antioxidantcapacity using a new generation, more stable ABTS radical cation, Clinical Biochemistry, 2004; 37: 277–85
  15. Aycicek A., Erel O., Kocyigit A., Increased oxidative stres in infants exposed o passive smoking, Eur J Pediatr, 2005;164:775–8
  16. Aycicek A., Varma M., Ahmet K., Abdurrahim K., Erel O., Maternal active or passive smoking causes oxidative stress in placental tissue, Eur J Pediatr, 2011 May; 170: 645-51. 18.