Journals / Dicle Tıp Dergisi / 2011 / Cilt: 38 - Sayı: 4

The protective effects of omega-3 fatty acids on rat testicular tissue

Omega-3 yağ asitlerinin sıçan testis dokusu üzerine koruyucu etkileri

Pages
382–386
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: We concluded that ω-3 fatty acid had favorable effects in rat testis tissue by preventing oxidative damage and increasing the level of testosterone.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: In this study, the protective effect of omega-3 fatty acids on testicular tissue was aimed to investigate at biochemical levels.
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Key Findings & Data:Materials and methods: Totally, 16 adult male Wistar rats were divided into two groups.
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Conclusion & Impact:Conclusion: We concluded that ω-3 fatty acid had favorable effects in rat testis tissue by preventing oxidative damage and increasing the level of testosterone.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: In this study, the protective effect of omega-3 fatty acids on testicular tissue was aimed to investigate at biochemical levels. Materials and methods: Totally, 16 adult male Wistar rats were divided into two groups. Rats in Group I were used as control and only saline was given by intragastric gavage. Rats in Group II, 400 mg/kg dose ω-3 fatty acids were given daily by intragastric gavage. At the end of the six-week experimental period, all rats were killed by decapitation. The testicular tissue specimens taken from animals, superoxide dismutase, glutathione peroxidase, malondialdehyde, enzyme activities were measured spectrophotometrically. In addition, blood testosterone levels were examined. Results: In our study, ω-3 fatty acids in rats were given a statistically significant increase in the levels of superoxide dismutase, and glutathione peroxidase a statistically significant decrease in malondialdehyde levels were determined when compared to control group. In addition, ω-3 fatty acids in rats given a statistically significant increase in blood testosterone levels were observed. Conclusion: We concluded that ω-3 fatty acid had favorable effects in rat testis tissue by preventing oxidative damage and increasing the level of testosterone.

Özet

Amaç: Bu çalışmada testis dokusu üzerine omega-3 yağ asitlerinin koruyucu etkisi biyokimyasal düzeyde araştırılması amaçlandı. Gereç ve yöntem: Toplam 16 adet Wistar-Albino cinsi erişkin erkek sıçan 2 gruba ayrılarak incelendi. Grup I’deki sıçanlar kontrol grubu olarak alındı ve sadece intragastrik gavaj ile serum fizyolojik verildi. Grup II’deki sıçanlara ise, 400 mg/kg dozundaki ω-3 yağ asiti intragastrik gavaj yoluyla günlük olarak verildi. Altı haftalık deney süresi sonunda tüm sıçanlar dekapitasyon yöntemiyle öldürüldü. Hayvanlardan alınan testis doku örneklerinde superoksit dismutaz, Glutatyon peroksidaz, malondialdehit aktiviteleri spektrofotometrik olarak tayin edildi. Ayrıca kan testosteron düzeyleri incelendi. Bulgular: Çalışmamızda, ω-3 yağ asidi verilen sıçanlarda superoksit dismutaz, Glutatyon peroksidaz düzeyleri istatistiksel olarak anlamlı bir şekilde artarken, malondialdehit düzeylerinde kontrol grubuna göre istatistiksel olarak anlamlı bir düşüş tespit edildi. Ayrıca omega-3 yağ asidi verilen sıçanların kan testosteron düzeylerinde istatistiksel olarak anlamlı bir artış görüldü. Sonuç: Sonuç olarak omega-3 yağ asitlerinin sıçan testis dokusunda gerek oksidatif hasarı önleyerek, gerekse testosteron düzeyini artırarak olumlu etki gösterdiği tespit edildi.

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Cite This Article

ZARARSIZ, İ., KUŞ, İ., DAVARER, M., KUŞ, M. A., KAMAN, D., SARSILMAZ, M. (2011). The protective effects of omega-3 fatty acids on rat testicular tissue. Dicle Tıp Dergisi, Cilt: 38 - Sayı: 4, 382–386. https://ajindex.com/articles/349852

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References (15)

  1. Nordoy A. Is there a rational use for n-3 fatty acids (fish oils) in clinical medicine? Drugs 1991; 42(3):331-42
  2. Bourre JM, Bonneil M, Chaudiere J, Clement M, Dumont O, Durand G. Structural and functional importance of dietary polyunsaturated fatty acids in the nervous system. Adv Exp Med Biol 1992;318(1):211-29
  3. Masters C. Omega-3 fatty acids and the peroxisome. Mol Cell Biochem 1996;165(2):83-93
  4. Zararsız İ, Kuş İ, Akpolat N, Songur A, Ögetürk M, Sarsılmaz M. Protective Effects of Omega-3 Essential Fatty Acids Against Formaldehyde-Induced Neuronal Damage in Prefrontal Cortex of Rats. Cell Biochem Funct 2006;.24(3):237-44
  5. Zararsiz I, Sonmez MF, Yilmaz HR, et al. Effects of omega-3 essential fatty acids against formaldehyde induced nephropathy in rats. Toxicol Ind Health 2006b;22(5):223-9
  6. Gamoh S, Hashimoto M, Sugioka K, Shahdat Hossain M, Hata N, Misawa Y, Masumura S. Chronic administration of docosahexaenoic acid improves reference memory-related learning ability in young rats. Neuroscience 1999;93(1):237-41
  7. Zararsız İ, Sönmez MF, Yılmaz HR, Pekmez H, Kuş İ, Sarsılmaz M. Sıçanlarda formaldehit uygulamasıyla akciğerlerde oluşan hasar üzerine omega-3 yağ asitlerinin koruyucu etkisi. Selçuk Tıp Dergisi 2004;20(3):93-8
  8. Sarsılmaz M, Songur A, Ozyurt H, et al. Potential role of dietary ω-3 essential fatty acids on some oxidant/antioxidant parameters in rats’ corpus striatum. Prostaglandins Leukot Essent Fatty Acids 2003;69(4):253-9
  9. Wills E D. The role of Dietary Components in Oxidative Stress in Tissues. Sies H (Ed.). Academic Press. Orlando, 1985; s: 197-220
  10. Yılmaz, H.R, Songur A, Özyurt B, Zararsız İ, Sarsılmaz M. The effects of n-3 polyunsaturated fatty acids by gavage on some metabolic enzymes of rat liver. Prostaglandins Leukot Essent Fatty Acids 2004;71(2):131-5
  11. Parker G, Gibson N.A, Brotchie H, Heruc G, Rees A.M, Pavlovic D.H. Omega-3 Fatty Acids and Mood Disorders. Am J Psychiatry 2006;163(6): 969-78
  12. Saravanan P, Davidson NC, Schmidt EB, Calder PC. Cardiovascular effects of marine omega-3 fatty acids. The Lancet 2010; 376 (9740): 540-50
  13. Comhaire FH, Mahmoud A. The role of food supplements in the treatment of the infertile man. Reprod Biomed Online 2003;7(4): 385-91
  14. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hidroxynonenal. Methods Enzymol 1990;186(1):407-21
  15. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide