Journals / Dicle Tıp Dergisi / 2016 / Cilt: 43 - Sayı: 2

The Investigation of Pathogenic E. coli serogroups in Patients with Diarrhea

Diyareli Hastalarda Patojenik E. coli Serogruplarının Araştırılması

Pages
205–211
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The main way to prevent diarrheal E. coli infections is to obey the hygiene rules.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Diarrheal diseases express a major health problem especially in developing countries.
📊
Key Findings & Data:In this study, it was aimed to determine the presence of bacterial enteropathogens especially diarrheagenic Escherichia coli serogroups in stool samples taken from patients with diarrhea in our geographic region.Methods: 343 stool samples were collected from the patients who were diarrhea.
💡
Conclusion & Impact:The main way to prevent diarrheal E. coli infections is to obey the hygiene rules.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Diarrheal diseases express a major health problem especially in developing countries. The real reasons of diarrheal disease are largely due to low hygiene or sanitation as well as low budgets of primary and secondary health care. In this study, it was aimed to determine the presence of bacterial enteropathogens especially diarrheagenic Escherichia coli serogroups in stool samples taken from patients with diarrhea in our geographic region.Methods: 343 stool samples were collected from the patients who were diarrhea. Stool samples were subjected to macroscopic and microscopic examinations, and then were cultured into EMB Agar, MacConkey Agar and Selenite F to isolate and distinguish E.coli from other intestinal pathogens. Finally, all isolated E.coli species were identified by using specific antisera. Results: 343 (156 female, 187 male) stool samples were bacteriologically and parasitologically examined. Only E. coli presence was detected in 262 (76.4%) samples. 77 (29.4%) of total isolated 262 E.coli strains were identified with latex agglutination test. Most common EHEC, EPEC, ETEC and EIEC strains were detected as following; O26, O55, O128 and O152 respectively. E.coli O157:H7 serovar was not detected.Conclusion: As a consequent, just usage of O antisera (except H7) is not adequate to detect all the pathogenic bacteria. However, determination of bacterial serogroups which often seen in a region may lead to draw the attention of the clinicians on these bacteria and provide an opportunity for more accurate diagnosis and treatment. The main way to prevent diarrheal E. coli infections is to obey the hygiene rules.

Özet

Amaç: Diyare ile ilişkili hastalıklar başta gelişmekte olan ülkeler olmak üzere birçok ülkede ciddi bir sağlık problemidir. Diyare ile ilişkili hastalıkların gerçek nedeni, birincil ve ikincil sağlık hizmetlerinin düşük bütçelerinin yanı sıra sanitasyon ve kötü hijyen olarak tespit edilmiştir. Bu çalışmada, bölgemizdeki ishalli hastalardan alınan dışkı örneklerinde, başta ishal oluşturan Escherichia coli suşları olmak üzere bakteriyel enteropatojenlerin varlığının tespit edilmesi amaçlanmıştır.Yöntemler: İshal şikayeti olan 343 hastadan dışkı örnekleri toplandı. Toplanan dışkı örnekleri ilk olarak makroskobik ve mikroskobik incelemeye tabi tutuldu. Ardından, örneklerin EMB Agar, MacConkey Agar and Selenite F besiyerlerine ekim işlemleri gerçekleştirildi. Son olarak, E.coli bakterileri spesifik antiserumlar kullanılarak tanımlandı. Bulgular: Parazitolojik ve bakteriyolojik incelemeye tabi tutulan 343 dışkı örneğinin 156'sı kadın, 187'si erkek hastalara aitti. 262 dışkı örneğinde (%76,4) yalnızca E.coli bakterisi tespit edildi. E.coli tespit edilen 262 hastanın 77'sinde (%29,4) lateks aglütinasyon yöntemi ile sınıflandırma yapıldı. En çok tespit edilen EHEC, EPEC, ETEC ve EIEC suşları sırasıyla O26, O55, O128 ve O152 alt serotipleri olarak tanımlandı. Bu çalışmada, E.coli O157:H7 suşu tespit edilmedi.Sonuç: Sonuç olarak, sadece O antiserumunun kullanılması tüm patojenik bakterilerin tanımlanması için yeterli olmamaktadır. Ancak, bölgemizdeki bakteriyel serogrupların tanımlanması, konu hakkında klinisyenlerin dikkatini çekecek ve doğru tanı ve tedavi konusunda daha doğru sonuçlara ulaşılmasına olanak sağlayacaktır. İshal oluşturan E.coli suşlarının sebep olduğu infeksiyonların önüne geçebilmenin en temel yolu, hijyen kurallarına özen göstermektir.

🔖

Cite This Article

CALİK, Z. Z., KARAMEŞE, M., AKTAŞ, O. (2016). The Investigation of Pathogenic E. coli serogroups in Patients with Diarrhea. Dicle Tıp Dergisi, Cilt: 43 - Sayı: 2, 205–211. https://ajindex.com/articles/348834

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Assis FE, Wolf S, Surek M, et al. Impact of Aeromonas and diarrheagenic Escherichia coli screening in patients with diarrhea in Paraná, southern Brazil. J Infect Dev Ctries 2014;8:1609-1614
  2. World Health Organization. Diarrheal Diseases. Geneva: WHO. Available: http://www.who.int/vaccine_research/ diseases/diarrhoeal/en/index.html Accessed: 12 January 2015
  3. Sjöling A, Sadeghipoorjahromi L, Novak D, et al. Detection of major diarrheagenic bacterial pathogens by multiplex PCR panels. Microbiol Res 2014;5013:151-157
  4. Marcos LA, DuPont HL. Advances in defining etiology and new therapeutic approaches in acute diarrhea. J Infect 2007;55:385-393
  5. Tan J, File T, Salata R, et al. Infectious diseases. Expert guide series. The American College of Physicians. Philadelphia: Versa Press 2008;95
  6. Rey A, Verjan N, Ferguson HW, et al. Pathogenesis of Aeromonas hydrophila strain KJ99 infection and its extracellular products in two species of fish. Vet Rec 2009;164:493-499
  7. Chen Y, Chen X, Zheng S, et al. Serotypes, genotypes and antimicrobial resistance patterns of human diarrhoeagenic Escherichia coli isolates circulating in southeastern China. Clin Microbiol Infect 2014;20:52-58
  8. Turk H, Findik D. Investigation of rotavirus and adenovirus antigens in patients with acute gastroenteritis. J Clin Exp Invest 2014;5:256-260
  9. Leclerc H, Schwartzbrod L, Dei-Cas E. Microbial agents associated with waterborne diseases. Crit Rev Microbiol 2002;28:371-409
  10. Guerrant RL, Hughes JM, Lima NL, et al. Diarrhea in developed and developing countries: magnitude, special settings, and etiologies. Rev Infect Dis 1990;1:41-50
  11. Bayram Y, Parlak M, Çıkman A. The prevalence of Giardia intestinalis and Entamoeba histolytica/dispar in Van Regional Training and Research Hospital: A four-year monitoring. Dicle Med J 2013;40:40-44
  12. Robins-Browne RM, Levine MM, Rowe B, et al. Failure to detect conventional enterotoxins in classical enteropathogenic (serotyped) Escherichia coli strains of proven pathogenicity. Infect Immun 1982;38:798-801
  13. Atmar RL, Estes MK. Diagnosis of noncultivatable gastroenteritis viruses, the human caliciviruses. Clin Microbiol Rev 2001;14:15-37
  14. Liptakova A, Siegfried L, Rosocha J, et al. A family outbreak of haemolyticuraemic syndrome and haemorrhagic colitis caused by verocytotoxigenic Escherichia coli O157 from unpasteurised cow's milk in. Clin Microbiol Infect 2004;10:576-578
  15. Havelaar AH, Van Duynhoven YT, Nauta MJ, et al. D