BIOCHEMICAL ANALYSIS FOR NEUROPROTECTIVE EFFECTS OF GANODERMA LUCIDUM IN EXPERIMENTAL RAT SPINAL CORD TRAUMA MODEL

The results of this biochemical study is supporting the previous studies and showing that GL has the potential of reducing posttraumaticoxidative damage in the spinal cord when given at the appropriate dose.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Injury of the spinal cord is studied in two separate mechanisms as primary and secondary injuries.

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Key Findings & Data:During the secondary injury,spinal cord damage and related neurological defects could increase mostly because of oxidative damage.

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Conclusion & Impact:The results of this biochemical study is supporting the previous studies and showing that GL has the potential of reducing posttraumaticoxidative damage in the spinal cord when given at the appropriate dose.

🔬Design:Modeling & Simulation

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Özet

Objective: Injury of the spinal cord is studied in two separate mechanisms as primary and secondary injuries. During the secondary injury,spinal cord damage and related neurological defects could increase mostly because of oxidative damage. Treatments targeting this process arepromising for reducing neuronal damage. Ganoderma lucidum (GL) has the potential to suppress the inflammatory response and oxidative stress.The aim of this study is to determine the neuroprotective effect of traditional GL hot-water extract during the secondary spinal cord injury (SCI)period on an experimental rat spinal trauma model by measuring the biochemical parameters.Materials and Methods: A total of 34 rats were distributed randomly into 4 groups as trauma, vehicle, low-dose medication group (low-DMG)and high-dose medication group (high-DMG). A modified Walsh-Tator clip was applied extradurally to form an experimental SCI model. GL liquidextract was performed in medication groups with low and high (10 times higher) oral doses. Spinal cord specimens were collected after 5 daysof treatment for biochemical analysis.Results: In the low-DMG, both diphenypicrylhydrasyl (DPPH) and malondialdehyde (MDA) values were found statistically negligible whencompared with the trauma group. Comparison of the Low-DMG and vehicle group showed a significant change in DPPH value, but an insignificantchange in MDA value. A statistically significant positive change in both DPPH and MDA values was found in High-DMG when compared to traumaand vehicle groups.Conclusion: Prevention of secondary SCI is very important, since the neurological condition of the patients may get worse during this period.Dose-dependent positive results were obtained in the favor of GL in terms of both antioxidant efficacy and prevention of lipid peroxidation afterSCI. The results of this biochemical study is supporting the previous studies and showing that GL has the potential of reducing posttraumaticoxidative damage in the spinal cord when given at the appropriate dose.

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Cite This Article

DİVANLIOĞLU, D., SALİHOĞLU, E. M., KORKMAZ, M., SEÇEN, A. E., ÖCAL, Ö., GÜNERHAN, G., BELEN, D., DALGIÇ, A. (2021). BIOCHEMICAL ANALYSIS FOR NEUROPROTECTIVE EFFECTS OF GANODERMA LUCIDUM IN EXPERIMENTAL RAT SPINAL CORD TRAUMA MODEL. Journal of Turkish Spinal Surgery, Cilt: 32 - Sayı: 3, 116–122. https://ajindex.com/articles/347538

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References (16)

Tator CH, Fehlings MG. Review of the secondary injury theory of acute spinal cord trauma with emphasis on vascular mechanisms. J Neurosurg. 1991;75:15-26
Fehlings MG, Sekhon LH, Tator C. The role and timing of decompression in acute spinal cord injury: what do we know? What should we do? Spine (Phila Pa 1976). 2001;26(Suppl 24):S101-110
Hardman J. Cerebrospinal Trauma. In: Davis R, Robertson D (Eds.). Textbook of Neuropathology. Second edition, Williams and Wilkins, Baltimore 1991;pp:962-1003
Crowe MJ, Bresnahan JC, Shuman SL, Masters JN, Beattie MS. Apoptosis and delayed degeneration after spinal cord injury in rats and monkeys. Nat Med. 1997;3:73-6
İplikcioglu C. Omurilik yaralanmasının fizyopatolojisi. İçinde: Özer F, Zileli M (Eds.). Omurilik ve Omurga Cerrahisi. Birinci basım, Saray Medikal Yayıncılık, İzmir 2002;pp:459-65
Popovich PG, Longbrake EE. Can the immune system be harnessed to repair the CNS? Nat Rev Neurosci. 2008;9:481-93
Rossignol S, Schwab M, Schwartz M, Fehlings MG. Spinal cord injury: time to move? J Neurosci. 2007;27:11782-92
Tator C. Neurosurgery. In: Wilkins R, Rengachary S (Eds.). Second edition, McGraw-Hill, New York 1996;pp:2847-59
Popovich PG, Jones TB. Manipulating neuroinflammatory reactions in the injured spinal cord: back to basics. Trends Pharmacol Sci. 2003;24:13-7
Erden M. Serbest radikaller. Turkiye Klinikleri J Med Sci. 1992;12:201-7
Sakamoto A, Ohnishi ST, Ohnishi T, Ogawa R. Relationship between free radical production and lipid peroxidation during ischemiareperfusion injury in the rat brain. Brain Res. 1991;554:186-92
Valenzuela A. The biological significance of malondialdehyde determination in the assessment of tissue oxidative stress. Life Sci. 1991;48:301-9
Chang ST, Buswell JA. Medicinal mushrooms-a prominent source of nutriceuticals for the 21st century. Curr Top Nutraceutical Res. 2003;1:257-80
Wachtel-Galor S, Yuen J, Buswell JA, Benzie IFF. Ganoderma lucidum (Lingzhi or Reishi): A Medicinal Mushroom. In: Benzie IFF, WachtelGalor S (Eds.). Herbal Medicine: Biomolecular and Clinical Aspects. Second edition, CRC Press/Taylor & Francis, Boca Raton (FL) 2011
Dzubak P, Hajduch M, Vydra D, Hustova A, Kvasnica M, Biedermann D, et al. Pharmacological activities of natural triterpenoids and their therapeutic implications. Nat Prod Rep. 2006;23:394-411
Lin Z-B. Cellular and molecular mechanisms of immuno-modulation by Ganoderma lucidum. J Pharmacol Sc