Journals / Türk Tarım - Gıda Bilim ve Teknoloji dergisi / 2021 / Cilt: 9 - Sayı: 4

Karyotypical Identification of Some Important Alfalfa (Medicago sativa L.) Lines in Turkey

Pages
740–744
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results of the karyotype asymmetry index analysis showed that intrachromosomal asymmetry is higher than interchromosomal asymmetry in the karyotypes of alfalfa lines.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Chromosome line and characterization of nine Turkish alfalfa (Medicago sativa L.) lines were investigated using karyological techniques in this study.
📊
Key Findings & Data:Root tips were obtained from germinated alfalfa seeds in petri dishes at room temperature (25°C) for visualizing somatic chromosomes.
💡
Conclusion & Impact:The results of the karyotype asymmetry index analysis showed that intrachromosomal asymmetry is higher than interchromosomal asymmetry in the karyotypes of alfalfa lines.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Özet

Chromosome line and characterization of nine Turkish alfalfa (Medicago sativa L.) lines were investigated using karyological techniques in this study. Root tips were obtained from germinated alfalfa seeds in petri dishes at room temperature (25°C) for visualizing somatic chromosomes. The chromosome numbers of nine alfalfa lines were determined as 2n=4x=32. The karyotype formula of 2n=32= 30m+ 2sm (30 median + 2 submedian) were noted for Line 1, 2and 3. Whereas, Line 4, 6, 8, 9 showed the karyotype formula of 2n=32=32m (32 median). The karyotype formula of Line 5 and 7 were 2n=32=28m+4sm (28 median + 4 submedian). Satellite chromosome pairs were observed from Line 5. The results of the karyotype asymmetry index analysis showed that intrachromosomal asymmetry is higher than interchromosomal asymmetry in the karyotypes of alfalfa lines.

🔖

Cite This Article

ÖZKAN, U., BENLİOĞLU, B. (2021). Karyotypical Identification of Some Important Alfalfa (Medicago sativa L.) Lines in Turkey. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, Cilt: 9 - Sayı: 4, 740–744. https://ajindex.com/articles/341276

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Albayrak S, Turk M, Sevimay CS, Anakhatoon E. 2015. Karyotype Characterization of Alfalfa (Medicago sativa L.) Collected from Lake Regions of Turkey. Scientific Papers. Series A. Agronomy, 58: 351-353.
  2. Benlioglu, B. 2020. Cytogenetic Diversity and Characterization of Vicia sativa Subspecies. Legume Research. Doi: 10.18805/LR-567. Cluster PD, Calderini O, Pupilli F, Crea F, Damiani F, Arcioni S. 1996. The fate of ribosomal genes in three interspecific somatic hybrids of Medicago sativa: three different outcomes including the rapid amplification of new spacer-length variants. Theor. Appl. Genetics, 93: 801–808. Falistocco E. 1987. Cytogenetic investigations and karyological relationships of two Medicago: Medicago sativa L. (alfalfa) and Medicago arborea L. Caryologia, 40: 339–346.
  3. Farshadfar M, Boloorchıan F, Safarı H, Shırvanı H. 2017. Analysis of genetic and cytogenetic variations between alfalfa (Medicago sativa L.) genotypes in Iran. Iranian Journal of Field Crop Science, Volume 48 (3): 695- 708. Fyad-Lameche FZ, Iantcheva A, Siljak-Yakovlev S, Brown SC. 2016. Chromosome number, genome size, seed storage protein profile and competence for direct somatic embryo formation in Algerian annual Medicago species. Plant Cell, Tissue and Organ Culture, 124(3): 531-540. Genckan MS. 1983.
  4. Forage Crops. Ege University, Faculty of Agriculture. No: 464-519. pg: 5-6, Izmir. (In Turkish) Huziwara Y. 1962. Karyotype analysis in some genera of Compositae. VIII. Further studies on the chromosomes of Aster. American Journal of Botany, 49.2:116-119. Lapiņa L, Grauda D, Rashal I. 2011. Characterization of Latvian alfalfa Medicago sativa genetic resources. Acta Biol. Univ. Daugavp, 11: 134-140.
  5. Levan A, Fredga K, Sandberg AA. 1964. Nomenclature for centromeric position on chromosomes. Hereditas, 52: 201- 220. Mariani A, Pupilli F, Calderini O. 1996. Cytological and molecular analysis of annual species of the genus Medicago. Canadian Journal of Botany, 74 (2): 299-307.
  6. Martin E, Yıldız HK, Kahraman A, Binzat OK, Eroglu HE. 2018. Detailed chromosome measurements and karyotype asymmetry of some Vicia (Fabaceae) taxa from Turkey. Caryologia, 71(3): 224-232.
  7. McCoy TJ, Bingham ET. 1988. Cytology and cytogenetics of alfalfa. In: alfalfa and alfalfa improvement. Agron. Monogr. 29. ASA, CSSA and SSSA. Madison, WI, pp 737–776.
  8. Peruzzi L, Eroglu E. 2013. Karyotype asymmetry: again, how to measure and what to measure? Comparative Cytogenetics, 7: 1-9.
  9. Quiros CF, Bauchan GR. 1988. The genu