Journals / Van Tıp Dergisi / 2007 / Cilt: 14 - Sayı: 2

The investigation of the causative agents with culture and fluorescence antibody methods in adult patients with community-acquired pneumonia

Pnömoni tanılı erişkin hastalarda kültür ve floresan antikor yöntemleriyle etkenlerin araştırılması

Pages
41–45
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: In pneumoniae, which is an important health problem with high mortality and morbidity rates, it is necessary to diagnose with multiple methods for correct and early diagnosis of the disease.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: In this study was designed to investigate causative agents in patients with community-acquired pneumonia by use of culture and immunofluorescence antibodies.
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Key Findings & Data:Methods: According to this purpose in 50 patients with clinically diagnosed pneumonia, blood cultures using BACTEC 9120 system (Beckton Dickinson - USA), sputum cultures with conventional methods were performed and isolated agents were identified with Sceptor panels (Becton Dickinson - USA).
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Conclusion & Impact:Conclusion: In pneumoniae, which is an important health problem with high mortality and morbidity rates, it is necessary to diagnose with multiple methods for correct and early diagnosis of the disease.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Floresans antikor tekniği · indirekt · Erişkin

Abstract

Aim: In this study was designed to investigate causative agents in patients with community-acquired pneumonia by use of culture and immunofluorescence antibodies. Methods: According to this purpose in 50 patients with clinically diagnosed pneumonia, blood cultures using BACTEC 9120 system (Beckton Dickinson - USA), sputum cultures with conventional methods were performed and isolated agents were identified with Sceptor panels (Becton Dickinson - USA). Also from the serums of the patients, using indirect fluorescence antibody methods (Pneumo Slide test-Poligono Industrial Dos De Octubre) IgM antibody against 9 pneumonia agents were investigated. Results: From a total of 50 patients the diagnosis was determined in 13 (26 %) with blood culture, in 6 (12 %) with sputum culture and in 26 (56 %) with indirect fluorescence antibody methods. In the end of the three study, rates of isolation of specific pathogens responsible for community-acquired pneumonia in our region were as follows: Streptococcus pneumoniae, 18 %, Legionella pneumophilia, 12 %, Mycoplasma pneumoniae 12 %, Influenza A 10 %, Haemophilus influenzae 8 %, Staphylococcus aureus 6 %, Adenovirus 6 %, Klebsiella pneumoniae 4 %, Parainfluenza 4 %, Respiratory syncytial virus 2 %, Influenza B 2 %, Coxiella burnetii 2 %, Chlamydia pneumoniae 2 %, Staphylococcus epidermis 2 %. With those methods couldn’t have been identified specific pathogen in 10 % of patients. Conclusion: In pneumoniae, which is an important health problem with high mortality and morbidity rates, it is necessary to diagnose with multiple methods for correct and early diagnosis of the disease.

Özet

Amaç: Bu çalışmada, toplum kökenli pnömoni tanısı konulan hastalarda kültür ve indirekt floresan antikor yöntemi ile etkenlerin araştırılması amaçlanmıştır. Yöntem: Klinik olarak pnömoni tanısı konmu 50 hastadanın klasik yöntemler kullanılarak balgam kültürleri yapılmış , Bactec 9120 sistemi ile kan kültürleri (Becton Dickinson- USA) alınmış , izole edilen etkenler Sceptor (Becton Dickinson- USA) panelleri ile identifıye edilmiştir. Ayrıca hastalardan alınan serum örneklerinde indirekt floresan antikor yöntemi (Pneumoslide test - Poligono Industrial Dos De Octubre) ile 9 pnömoni etkenine karşı IgM antikorları araştırılmıştır. Bulgular: Çalışma kapsamındaki 50 pnömonili hastanın 13 (% 26)’ünde kan kültürü, 6 (% 12)’sında balgam kültürü, 26 (% 52)’sında ise indirekt floresan antikor yöntemi ile tanı konulmuştur. Bu üç yöntemle yapılan çalışma sonucunda, bölgemizde erişkin yaş grubunda gözlenen toplum kökenli pnömoni vakalarının % 18’inde Streptococcus pneumoniae, % 12’sinde Legionella pneumophilia, % 12’sinde Mycoplasma pneumoniae, % 10’unda Influenza A, % 8’inde Haemophilus influenzae, % 6’sında Staphylococcus aureus ve Adenovirus, % 4’ünde Klebsiella pneumoniae ve Parainfluenza, % 2’sinde Respiratory syncytial virus, Influenza B, Coxiella burnetii, Chlamydia pneumoniae ve Staphylococcus epidermidis’in etken oldukları saptanmıştır. Hastaların % 10’unda ise bu yöntemlerle etken saptanamamıştır. Sonuç: Mortalitesi ve morbiditesi yüksek bir sağlık sorunu olan pnömonilerde, hastalığın doğru ve erken tanısı açısından birden fazla yöntemle tanıya gidilmesi önemlidir.

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Cite This Article

BOZKURT, H., GÜDÜCÜOĞLU, H., ANDİÇ, Ş., BERKTAŞ, M., ÇİFTCİ, İ. H., ÖZBAY, B. (2007). The investigation of the causative agents with culture and fluorescence antibody methods in adult patients with community-acquired pneumonia. Van Tıp Dergisi, Cilt: 14 - Sayı: 2, 41–45. https://ajindex.com/articles/334218

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