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Journals / Veteriner Bilimleri Dergisi . Eurasian Journal of Veterinary Sciences / 2001 / Cilt: 17 - Sayı: 3

The studies on virus isolation from feces in calves

Yeni doğan buzağı ve danalarda gaitadan virus izolasyonu çalışmaları

Sibel YAVRU
Atilla ŞİMŞEK
Pages
51–58
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Serum neutralization 50 (SN50) values of seropositive animals were detected to be 1/14.1-1/531, 1/14.1-1/316 and 1/14.1-1/531 for BAV-1,2 and 3 respectively.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, 100 feces samples from 0-1 old year calves with clinical sign of respiratory and digestive systems, fever and acut diarrhae symptom in eight private managements of Konya and its arround were used for virus isolation studies in sensitive cell cultures and checked for BAV 1,2 and 3.
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Key Findings & Data:As a result of inoculation of 100 feces samples onto foetal calf kidney cell cultures, 2 viruses were isolated.
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Conclusion & Impact:Serum neutralization 50 (SN50) values of seropositive animals were detected to be 1/14.1-1/531, 1/14.1-1/316 and 1/14.1-1/531 for BAV-1,2 and 3 respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:izolasyon · Adenoviridae · buzağı

Abstract

In this study, 100 feces samples from 0-1 old year calves with clinical sign of respiratory and digestive systems, fever and acut diarrhae symptom in eight private managements of Konya and its arround were used for virus isolation studies in sensitive cell cultures and checked for BAV 1,2 and 3. As a result of inoculation of 100 feces samples onto foetal calf kidney cell cultures, 2 viruses were isolated. The isolated viruses were identified as BAV-1 by cross microneutralization test with rabbit hyperimmun sera. Blood serum samples from these animals were tested using microneutralization test for neutralizing antibody to BAV 1, 2 and 3. The presence of neutralizing antibody was detected in 17 out of 100 blood sera (17%) against BAV-1, in 21 (21%) against BAV-2 and in 23 (23%) against BAV-3. Serum neutralization 50 (SN50) values of seropositive animals were detected to be 1/14.1-1/531, 1/14.1-1/316 and 1/14.1-1/531 for BAV-1,2 and 3 respectively.

Özet

Bu araştırmada; Konya ve çevresinde bulunan sekiz adet özel işletmeye ait solunum ve sindirim sistemi klinik belirtileri gösteren vücut ısısı yüksek ve diyareli 0-1 yaş arası buzağı ve danalardan alınan 100 adet gaita örneği Bovine Adenovirus tip1, 2 ve 3 (BAV-1, 2 ve 3) yönünden kontrol edilerek, duyarlı hücre kültürlerinde virus izolasyonu çalışmaları yapıldı. İncelenen 100 gaita örneğinin fötal dana böbrek hücre kültürlerine inokulasyonu sonucu, 2 adet virus izole edildi. Tavşanlardan hazırlanan hiperimmun serum kullanılarak izole edilen virusların 2'si de çapraz mikronötralizasyon testi sonuçlarına göre BAV-1 olarak identifiye edildi. Gaita örneği alınan buzağılardan elde edilen kan serumlarında BAV-1, 2 ve 3'e karşı nötralizan antikor varlığı mikronötralizasyon testi ile araştırıldı. Hastalıklı hayvanlardan alınan 100 adet kan serumundan 17'inde (%17) BAV-1'e karşı, 21'inde (%21) BAV-2'ye karşı, 23'ünde (%23) BAV-3'e karşı nötralizan antikor varlığı ortaya konuldu. Pozitif bulunan hayvanların en düşük ve en yüksek serum nötralizasyon 50 (SN50) değerleri BAV-1, 2 ve 3 için sırasıyla 1/14.1-1/531 , 1/14.1-1/316 ve 1/14.1-1/531 olarak tespit edildi.

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Cite This Article

YAVRU, S., ŞİMŞEK, A. (2001). The studies on virus isolation from feces in calves. Veteriner Bilimleri Dergisi . Eurasian Journal of Veterinary Sciences, Cilt: 17 - Sayı: 3, 51–58. https://ajindex.com/articles/333640

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