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Journals / European Journal of Biology / 2019 / Cilt: 78 - Sayı: 2

Effects of Tempol in Lipopolysaccharide-Induced Liver Injury

Perihan Sinem SERİN
Aslı KANDİL
Huri BULUT
Tugba KASKAVALCİ
Erman Caner BULUT
Cihan DEMİRCİ-TANSEL
Pages
147–155
Publication Date
2019-12-06
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results may contribute to a better understanding of the role of tempol and basic mechanisms of underlying oxidative stressrelated liver injury for further investigations.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Sepsis leads to conditions such as inflammatory and anti-inflammatory process, circulatory abnormalities, cellular and humoral reactions.
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Key Findings & Data:Materials and Methods: Male Wistar rats were divided into four groups: Control, LPS (15 mg/kg), LPS + Tempol group (100 mg/kg Tempol, three hours after LPS administration) and Tempol (100 mg/kg).
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Conclusion & Impact:These results may contribute to a better understanding of the role of tempol and basic mechanisms of underlying oxidative stressrelated liver injury for further investigations.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Lipopolysaccharide · Liver · Tempol

Abstract

Objective: Sepsis leads to conditions such as inflammatory and anti-inflammatory process, circulatory abnormalities, cellular and humoral reactions. Endotoxin-induced oxidative stress causes injury in the liver. The aim of this study was to evaluate the effects of a radical scavenger Tempol in lipopolysaccharide (LPS)-induced liver injury in rats. Materials and Methods: Male Wistar rats were divided into four groups: Control, LPS (15 mg/kg), LPS + Tempol group (100 mg/kg Tempol, three hours after LPS administration) and Tempol (100 mg/kg). Blood glucose and body temperature were measured during the experiment. Superoxide dismutase (SOD), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and C-reactive protein (CRP) levels were measured in plasma or liver tissue. Furthermore, histopathological changes and myeloperoxidase-stained leukocytes infiltration were assessed in liver tissue. Results: LPS caused tissue damage and leukocytes infiltration, increased AST, ALT and CRP levels, and decreased body temperature, blood glucose and SOD levels. Tempol reduced AST and ALT levels and increased SOD levels. Tempol did not prevent tissue damage, leukocytes infiltration and increment of CRP levels. There were no changes in body temperature and blood glucose levels. Conclusion: The present study suggests that tempol may have antioxidant properties in LPS-induced liver injury. These results may contribute to a better understanding of the role of tempol and basic mechanisms of underlying oxidative stressrelated liver injury for further investigations.

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Cite This Article

SERİN, P. S., KANDİL, A., BULUT, H., KASKAVALCİ, T., BULUT, E. C., DEMİRCİ-TANSEL, C. (2019). Effects of Tempol in Lipopolysaccharide-Induced Liver Injury. European Journal of Biology, Cilt: 78 - Sayı: 2, 147–155. https://ajindex.com/articles/33190

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