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Journals / European Journal of Biology / 2019 / Cilt: 78 - Sayı: 2

Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758)

Sudeshna SARKER
Thangapalam Jawahar ABRAHAM
Pages
139–146
Publication Date
2019-12-06
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Adoption of good nursery practices and appropriate health management measures would help to minimize the development and spread of columnaris disease.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: This study assessed the pathogenicity of Flavobacterium columnare isolated from the gill-rot of catfish, Clarias batrachus in West Bengal, India.
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Key Findings & Data:F. columnare SGM4 was identified based on the morphological, phenotypic and genotypic characterization.
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Conclusion & Impact:Conclusion: Adoption of good nursery practices and appropriate health management measures would help to minimize the development and spread of columnaris disease.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Clarias batrachus · Gill-rot · Flavobacterium columnare

Abstract

Objective: This study assessed the pathogenicity of Flavobacterium columnare isolated from the gill-rot of catfish, Clarias batrachus in West Bengal, India. Materials and Methods: The diseased catfish were examined as per standard laboratory practices. F. columnare SGM4 was identified based on the morphological, phenotypic and genotypic characterization. Abrasion-immersion and agar-disc diffusion methods were followed to assess the pathogenicity and antibiotic sensitivity of F. columnare, respectively. Results: The diseased catfish had white gills, tail rot, body discolouration, saddle-back, peeled skin, emaciation, and inflamed kidney. The yellow-pigmented rhizoid colonies from the gills of catfish were identified as F. columnare. Phylogenetically, F. columnare SGM4 branched with F. columnare strains. In abrasion-immersion challenge experiments, F. columnare SGM4 induced considerable mortalities (45%) in C. batrachus at 7.2×106 cells/mL at 24-30 °C. In challenged catfish, it caused cutaneous lesions, tail rot, white patches on the gills and degeneration of internal organs. F. columnare strains were highly sensitive to broad-spectrum antibiotics except for sulphafurazole. Conclusion: Adoption of good nursery practices and appropriate health management measures would help to minimize the development and spread of columnaris disease.

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Cite This Article

SARKER, S., ABRAHAM, T. J. (2019). Isolation, Identification and Pathogenicity of Flavobacterium columnare SGM4 in Catfish Clarias batrachus (Linnaeus 1758). European Journal of Biology, Cilt: 78 - Sayı: 2, 139–146. https://ajindex.com/articles/33188

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