Journals / Türk Üroloji Dergisi/Turkish Journal of Urology / 2002 / Cilt: 28 - Sayı: 1

Assessment of epididymal sperm and ductus deferens histomorphology in medical sympathectomized rats

Medikal sempatektomi yapılan sıçanlarda duktus deferens histomorfolojisi ve epididimal sperm değerlendirmesi

Pages
12–15
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

We found significant histomorphological changes in the ductus deferens although sperm concentration and motility did not change in caput and cauda epididymis in sympathectomized rats.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study we aimed to investigate the histomorphological changes of the ductus deferens and the sperm density and motility in the caput and cauda epididymis of the medically sympathectomized rats.
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Key Findings & Data:Thirty mature male Spraque-Dawley rats (18 of them in study group and 12 in control group) were used in this study.
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Conclusion & Impact:We found significant histomorphological changes in the ductus deferens although sperm concentration and motility did not change in caput and cauda epididymis in sympathectomized rats.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Sempatektomi · Epididimis · Sıçanlar

Abstract

In this study we aimed to investigate the histomorphological changes of the ductus deferens and the sperm density and motility in the caput and cauda epididymis of the medically sympathectomized rats. Thirty mature male Spraque-Dawley rats (18 of them in study group and 12 in control group) were used in this study. Low dose (10 mg/kg/ day) intraperitoneal guanethidine (CIBA) injection was performed in the study group as well as serum physiologic injected in control group for 15 days. Orchiepididymectomy was performed bilaterally under ether anesthesia at 15th day. Specimens extracted were used for counting epididymal sperm cells on the right side and for histomorphological examination of ductus deferens on the left side. Although both of caput and caudal sperm concentrations were lower in subjects than controls, these were not significant (p>0.05). When the study group was compared with the control group for sperm motility, there were not any differences between these groups either in caput or in cauda (p>0.05). On the other hand diminishes of the muscle layer and epithelial height of ductus deference and an expansion of the lumen were observed in sympathectomized group. We found significant histomorphological changes in the ductus deferens although sperm concentration and motility did not change in caput and cauda epididymis in sympathectomized rats.

Özet

Bu çalışmamızda medikal sempatektomi yapılan sıçanlarda duktus deferensteki histomorfolojik değişikliklerle, kaput ve kauda epididimisteki sperm konsantrasyon ve hareketliliğindeki değişimin araştırılmasını amaçladık. Çalışmada 18'i çalışma grubunda ve 12'si kontrol grubunda olmak üzere toplam 30 adet erişkin Spraque-Dawley erkek sıçan kullanıldı. Çalışma grubuna 15 gün süreyle düşük doz (10mg/kg/gün) intraperitoneal guanethidin (CIBA), kontrol grubuna ise serum fizyolojik enjeksiyonu yapıldı. 15. günde eter anestezisi altında bilateral orşiepididimektomi yapıldı. Her iki grupta sağ taraftan çıkarılan spesimenler epididimal sperm hücresi sayımında, sol taraf ise vaz deferensin histomorfolojik incelemesinde kullanıldı. Kaput ve kaudadaki sperm sayıları çalışma grubunda kontrole göre daha düşük olmakla birlikte istatistiksel olarak anlamlı değildi (p>0.05). Her iki grup sperm hareketliliği yönünden karşılaştırıldığında hem kaput hem de kaudal sperm hücreleri arasında fark yoktu (p>0.05). Bu sonuçlara karşın sempatektomili grupta duktus deferens kas tabakası ve epitel yüksekliğinde azalma ve tümenlerinde genişleme gözlenmiştir. Sempatektomi yapılan sıçanlarda kaput ve kauda epididimisteki sperm yoğunluğu ve hareketliliğinde değişiklik saptanmazken duktus deferens histomorfolojisinde anlamlı değişiklikler bulunmuştur.

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Cite This Article

AYDINLIOĞLU, A., ODABAŞ, Ö., ÖZBEK, H., ERDOĞAN, E., GÖÇER, F., RAĞBETLİ, M. Ç. (2002). Assessment of epididymal sperm and ductus deferens histomorphology in medical sympathectomized rats. Türk Üroloji Dergisi/Turkish Journal of Urology, Cilt: 28 - Sayı: 1, 12–15. https://ajindex.com/articles/328863

References (1)

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