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Journals / Uludağ Üniversitesi Veteriner Fakültesi Dergisi / 2001 / Cilt: 20 - Sayı: 3

Effects of acethylecholin on mice inferior colliculus neurons

Fare colliculus inferior sinir hücreleri üzerine asotilkolinin etkileri

Ramazan BAL
Hayrullah KÖSE
Pages
45–50
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, acethylcholine increased the activity of inferior colliculus.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, it was aimed to study the effects of acethylcholine, a neurotransmitter, on neurons in the inferior colliculus.
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Key Findings & Data:Small amounts of current injections (0.05-0.15 nA) caused the cells to fire many action potentials.
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Conclusion & Impact:In conclusion, acethylcholine increased the activity of inferior colliculus.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:asetilkolin · sinir hücresi · acetylcholine

Abstract

In this study, it was aimed to study the effects of acethylcholine, a neurotransmitter, on neurons in the inferior colliculus. Intracellular recordings using the whole cell patch clamp technique were made from neurons of mice inferior colliculus. Spontaneous activity and the evoked responses elicited with depolarizing and hyperpolarizing current pulses were recorded from neurons with regular firing pattern. Small amounts of current injections (0.05-0.15 nA) caused the cells to fire many action potentials. The same amount of currents following the preconditioning hyperpolarizing pulses ended up with less action potentials. The application of acetylcholine added in the perfusing solution (0.05 mM) depolarized the resting potential by 17 mV, increased the number of action potential occurred spontaneously and caused the membrane potential record to be uneven. In conclusion, acethylcholine increased the activity of inferior colliculus.

Özet

Bu çalışmada nörotransmitterlerden asetilkolinin colliculus inferiore sinir hücrelerini ne şekilde etkilediğinin belirlenmesi amaçlandı. Fare colliculus inferiore'unda yer alan sinir hücrelerinden tüm hücre patch clamp tekniğini kullanılarak hücre içi kayıtlar alındı. Düzenli ateşleme modeli oluşturan sinir hücrelerinin kendiliğinden oluşan aktiviteleri ve katot ile anot akımları uygulanarak uyarılan aktiviteleri kaydedildi. Küçük miktardaki katot akım uygulamaları (0.05-0.15 nA) ile çok sayıda aksiyon potansiyeli oluşturuldu. Dinlenme zar potansiyelinin anot akımlarla hiperpolarize edildikten sonra aynı miktarlarda katot akım uygulamalarına yanıt olarak, aynı hücrelerde uyarılan aksiyon potansiyel sayısının belirgin olarak azaldığı gözlendi. Perfüzyon solusyonu içerisinde uygulanan asetilkolinin (0.05 mM), dinlenme zar potansiyelini 17 mV değerinde depolarize ettiği, kendiliğinden oluşan aksiyon potansiyel sayısını belirgin olarak artırdığı, ve membran potansiyelinde dalgalanmalar meydana getirdiği gözlendi. Sonuç olarak, asetilkolinin colliculus inferiore'un sinir aktivitesini artırdığı belirlendi

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Cite This Article

BAL, R., KÖSE, H. (2001). Effects of acethylecholin on mice inferior colliculus neurons. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 20 - Sayı: 3, 45–50. https://ajindex.com/articles/326390

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References (1)

  1. Bal R, Janahmadi M, Green GG, Sanders DJ: Effect of calcium and calcium channel blockers on transient outward current of F76 and D1 neuronal soma membranes in the subesophageal ganglia of Helix aspersa. J Membr Biol 2000; 173:179-85 2. Bal R, Janahmadi M, Green GG, Sanders DJ: Two kinds of transient outward currents, I(A) and I(Adepol), in F76 and D1 soma membranes of the subesophageal ganglia of Helix aspersa. J Membr Biol 2001; 179: 71-8. 3. Bal R, Oertel D: Hyperpolarization-activated, mixed-cation current (I(h)) in octopus cells of the mammalian cochlear nucleus. J Neurophysiol 2000; 84: 806-17. 4. Bal R: Potassium currents in identified Helix aspersa neurones and in rat inferior colliculus neurones. PhD Thesis, University of Newcastle, 1998. 5. Brown DA, Adams PR: Muscarinic suppression of a novel voltage-sensitive K+ current in a vertebrate neurone. Nature 1980; 283: 673-6. 6. Coggan JS, Purnyn SL, Knoper SR, Kreulen DL: Muscarinic inhibition of two potassium currents in guinea-pig prevertebral neurons: differentiation by extracellular cesium. Neuroscience 1994; 59: 349- 61. 7. Coleman JR, Clerici WJ: Sources of projections to subdivisions of the inferior colliculus in the rat. J Comp Neurol 1987; 262: 215-226. 8. Connor JA, Stevens CF: Prediction of repetitive firing behavior from voltage clamp data on an isolated neuron soma. J Physiol 1971; 213: 31-53. 9. Faye-Lund H, Osen KK: Anatomy of the inferior colliculus in rat. Anat Embryol 1985; 171: 1-20. 10. Faye-Lund H: Projection from the inferior colliculus to the superior olivary complex in the al bino rat. Anat Embryol 1986; 175: 35-52. 11. Fujino K, Oertel D. Contrasting cholinergic responsiveness of T and D stellate cells in the cochlear nucleus of mice. Journal of Neuroscience 2001;(Abstract). 12. Glendenning KK, Baker BN: Neuroanatomical distribution of receptors for three potential inhibitory neurotransmitters in the brainstem auditory nuclei of the cat. J Comp Neurol 1988; 275: 288-308. 13. Guinan JJ: Physiology of olivocochlear efferents. In: The cochlea. Dallos P, Popper AN, Fay RR, eds. New York: Springer, 1996; 435-502. 14. Haj-Dahmane S, Andrade R: Muscarinic activation of a voltage-dependent cation nonselective current in rat association cortex. J Neurosci 1996; 16: 3848-61. 15. Hasuo H, Akasu T, Gallagher JP: Muscarine activates a nonselective cation current through a M3 muscarinic receptor subtype in rat dorsolateral septal nucleus neurons. J Neurophysiol 1996; 76: 2221-30. 16. Hil