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Journals / Uludağ Üniversitesi Veteriner Fakültesi Dergisi / 2004 / Cilt: 23 - Sayı: 1

Effect of partial hepatic ischemic-reperfusion injury on erythrocyte and plasma electrolyte balance, some serum biochemical parameters and liver cell histology in rats

Hepatik iskemi-reperfüzyon hasarının sıçanlarda alyuvar ve plazma elektrolit dengesi, bazı serum biyokimyasal parametreleri ve karaciğer hücre histolojisi üzerine etkisi

CENK AYDIN
Engin KENNERMAN
NURTEN GALİP
Murat YALÇIN
Füsun AK
Cüneyt KAYAALP
Pages
77–82
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was concluded that partial hepatic ischemia-reperfusion injury which causes severe hepato-cellular dysfunction induces changes also in the erythrocyte and plasma electrolyte balance.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the effects of partial hepatic ischemia reperfusion injury on erythrocyte and plasma electrolyte balance, some serum biochemical parameters and liver cell histology were evaluated.
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Key Findings & Data:Liver were subjected to 60 min of partial warm ischemia by clamping the hepatic pedicle which includes the A. hepatica, V. portae vessels and bile duct of the left and middle liver lobes.
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Conclusion & Impact:It was concluded that partial hepatic ischemia-reperfusion injury which causes severe hepato-cellular dysfunction induces changes also in the erythrocyte and plasma electrolyte balance.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:alyuvarlar · potasyum · doku bilimi

Abstract

In this study, the effects of partial hepatic ischemia reperfusion injury on erythrocyte and plasma electrolyte balance, some serum biochemical parameters and liver cell histology were evaluated. Liver were subjected to 60 min of partial warm ischemia by clamping the hepatic pedicle which includes the A. hepatica, V. portae vessels and bile duct of the left and middle liver lobes. After 30 minutes reperfusion, blood samples and liver specimens were taken for evaluation. Ischemia-reperfusjon injury induced increased (p<0.001) serum Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and decreased (p<0.05) plasma sodium (PNa) level and changes in hepatocyte histology. It was concluded that partial hepatic ischemia-reperfusion injury which causes severe hepato-cellular dysfunction induces changes also in the erythrocyte and plasma electrolyte balance.

Özet

Bu çalışmada parsiyel hepatik iskemi-reperfüzyon hasarının alyuvar ve plazma elektrolit dengesi, bazı serum biyokimyasal parametreleri ve karaciğer hücre histolojisi üzerine etkisi değerlendirildi. Karaciğerin sol ve orta loplarına ait A. hepatica, V. porta damarları ile safra kanalını içeren hepatik pedikül bağlanarak 60 dakika iskemi etkisine bırakıldı. Otuz dakikalık reperfüzyon sonrası, değerlendirme için kan ve karaciğer doku örnekleri alındı. İskemi-reperfüzyon hasarı serum Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) değerlerinde artışa (p<0.001), plazma sodyum (PNa) düzeyinde ise azalmaya (p<0.05) ve karaciğer hücre histolojisinde değişimlere neden oldu. Önemli düzeyde hepatosellüler fonksiyon bozukluğuna neden olan parsiyel hepatik iskemi-reperfüzyon hasarının alyuvar ve plazma elektrolit dengesini de değiştirebileceği sonucuna varıldı.

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Cite This Article

AYDIN, C., KENNERMAN, E., GALİP, N., YALÇIN, M., AK, F., KAYAALP, C. (2004). Effect of partial hepatic ischemic-reperfusion injury on erythrocyte and plasma electrolyte balance, some serum biochemical parameters and liver cell histology in rats. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 23 - Sayı: 1, 77–82. https://ajindex.com/articles/326316

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References (15)

  1. ABDENNEBİ, H.B., STEGHENS, J.P., MARGONARI, J., VIRIEUX, S.R., BOILLOT, O.: High-Na⁺ low-K UW cold storage solution reduces reperfusion injuries of the rat liver graft. Transp. Int., 1998, 11: 223-230
  2. DETROZ, B., HONORE, P., DENOISEUX, C., JACQUET, N.: Biology, Physiology and Physiopathology of Clamping during Liver Surgery, Hepato-Gastroenterology, 1998, 45: 359
  3. KAZUO, H., NISHIDA, T., SEIYAMA, A., UESHIMA, S., HAMADA, E., ITO, T., MATSUDA, H.: Recovery of blood flow and oxygen transport after temporary ischemia of rat liver. Am. J. Physiol., 1998, 275 (Heart Physiol. 44): H 243-249
  4. GRACE, P.A.: Ischemia-reperfusion injury. British Journal of Surgery, 1994, 81: 637-647
  5. HAIMOVICI, M.: Muscular, renal and metabolic complications of acute arterial occlusions. Myenephropathic syndrome. Surgery, 1989, 70: 737-57
  6. PARKS, D.A., GRANGER, D.N.: Contributions of ischemia and reperfusion to mucosal lesion formation. Am. J. Physiol., 1986, 250: G749-53
  7. CLEMENS, M.G., CHAUDRY, I.H., BAUE, A.E.: Alterations in hepatic water and electrolyte balance in sepsis. Arch. Surg., 1984, 199: 44-48
  8. PEARSE, F.J., WEISS, P.R., MILLER, J.R.: Effect of hemorrhage, anoxia and graded ischemia on hepatic gluconeogenesis. Surg. Forum, 1982, 244: R794-R800
  9. MOHAMMED, M.S.: Ion transport in circulatory and/or septic shock. Am. J. Physiol., 1987, 21: R809-R821
  10. WANG, J.Y., WANG, H., PANG, Z.O.: An experimental study on the mechanism of impairment of cell membrane during hemorrhagic shock in dogs. Zhonghua Nei ke Za Zhi, 1992 Feb, 31(2): 98-101
  11. ILLNER, H., SHIRES, G.T.: Changes in sodium, potassium and adenosine triphosphate contents of red blood cells in sepsis and septic shock. Circ. Shock, 1982, 9(3): 259-267
  12. AFIFI, F.: Some red blood cell abnormalities in patients with liver cirrhosis and ascites. I. Red blood cell sodium, potassium and water content. Tohoku J. Exp. Med., 1985 Jan, 145(1): 7-14
  13. ASTRUP, J., PRYTZ, H., THOMSEN, A.C., WESTRUP, M.: Red cell sodium and potassium contents in liver cirrhosis. Gastroenterology, 1980 Mar, 78(3): 530-534
  14. FLECHA, B.G., CUTRIN, J.C., BOVERIS, A.: Time course and mechanism of oxidative stress and tissue damage in rat liver subjected to in vivo ischemia-reperfusion. J. Clin. Invest. 1993, 91: 456-464
  15. GUPTA, M., DOBASHI, K., GREENE, E.L., ORAK, J.K., SINGH, I.: Studies on hepatic injury and antioxidant enzyme activities in rat sub cellular organelles following in vivo ischemia and reperfusio