Journals / Ulusal Travma ve Acil Cerrahi Dergisi / 2009 / Cilt: 15 - Sayı: 6

Omentektominin bakteriyel çoğalma üzerine etkisi: Deneysel çalışma

The influence of omentectomy on bacterial clearance: An experimental study

Pages
541–545
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Peritoneal infection may pursue a more severe course with increased bacterial entrance into the blood in the absence of the omentum.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:BACKGROUND The influence of an omentectomy on peritoneal defense mechanisms and its clinical consequences have not been fully elucidated.
📊
Key Findings & Data:Animals were assigned into five groups as follow: Group 1 (n=10), omentectomy alone; Group 2 (n=10), omentectomy + bacterial peritonitis; Group 3 (n=10), bacterial peritonitis alone; Group 4 (n=10), laparotomy alone; and Group 5 (n=10), sham group.
💡
Conclusion & Impact:Peritoneal infection may pursue a more severe course with increased bacterial entrance into the blood in the absence of the omentum.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Laparotomi · Omentum · Lenfoid doku

Abstract

BACKGROUND The influence of an omentectomy on peritoneal defense mechanisms and its clinical consequences have not been fully elucidated. In the present study, we aimed to investigate the influence of omentectomy on bacterial growth in blood and tissue specimen cultures in rats with experimental peritonitis. METHODS Fifty Wistar-Albino rats were included in the present study. Animals were assigned into five groups as follow: Group 1 (n=10), omentectomy alone; Group 2 (n=10), omentectomy + bacterial peritonitis; Group 3 (n=10), bacterial peritonitis alone; Group 4 (n=10), laparotomy alone; and Group 5 (n=10), sham group. Culture positivity rate and bacterial growth (colony forming units [CFU]/gram tissue) were assessed in mesenteric lymphoid tissue and venous blood of all animals. RESULTS Bacterial growth in lymphoid tissue was significantly higher in Groups 2 and 3 than others (both, p<0.05). CFU of Escherichia coli in lymphoid tissue was significantly higher in Group 2 than in Groups 1 and 3 (both, p<0.05/4). Blood culture positivity was significantly higher in Group 2 than the others (both, p<0.05). CONCLUSION The omentum has an important role in the host peritoneal defense system. Peritoneal infection may pursue a more severe course with increased bacterial entrance into the blood in the absence of the omentum.

Özet

AMAÇ Omentektominin periton defans mekanizmaları üzerine etkisi ve bunun klinik sonuçları her yönü ile incelenmiş değildir. Bu çalışmada, deneysel peritonit oluşturulan sıçanlarda omentektominin kan ve doku örneklerindeki bakteriyel çoğalma üzerine etkisini araştırmayı amaçladık. GEREÇ VE YÖNTEM Çalışmaya 50 adet Wistar Albino cinsi sıçan dahil edildi. Denekler 5 gruba ayrıldı: Grup 1 (n=10), yalnızca omentektomi; grup 2 (n=10), omentektomi+bakteriyel peritonit; grup 3 (n=10), yalnızca bakteriyel peritonit; grup 4 (n=10), yalnızca laparotomi; grup 5 (n=10), sham grubu. Tüm deneklerin mezenterik lenfoid doku ve venöz kanlarında kültür pozitifliği oranı ve üreyen bakteri koloni sayısı değerlendirildi. BULGULAR Grup 2 ve grup 3?teki bakteriyel çoğalma oranı diğer gruplardan anlamlı olarak daha yüksek idi (tümü için, p<0,05). Lenfoid dokuda üreyen Escherichia coli koloni sayısı grup 1 ve grup 3?ten anlamlı olarak daha yüksek idi (her ikisi için, p<0,05). Kan kültürü pozitifliği grup 2?de diğer gruplardan anlamlı olarak daha yüksek idi (tümü için, p<0,05). SONUÇ Omentum, periton defans sistemininde önemli bir role sahiptir. Omentumun olmaması halinde bakterilerin kana karışması artacağından peritonda enfeksiyon daha şiddetli bir seyir izleyebilir.

🔖

Cite This Article

UZUNKÖY, A., ÖZBİLGE, H., HOROZ, M. (2009). Omentektominin bakteriyel çoğalma üzerine etkisi: Deneysel çalışma. Ulusal Travma ve Acil Cerrahi Dergisi, Cilt: 15 - Sayı: 6, 541–545. https://ajindex.com/articles/324595

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Bosscha K, Hulstaert PF, Visser MR, van Vroonhoven TJ, van der Werken C. Open management of the abdomen and planned reoperations in severe bacterial peritonitis. Eur J Surg 2000;166:44-9
  2. Altaca G, Sayek I, Onat D, Gür D, Akalin E. Restoration of bactericidal activity of peritoneal fuid by cimetidine but not ranitidine or famotidine in burned mice. Eur J Surg 199;159:551-4
  3. Dunn DL, Simmons RL. Fibrin in peritonitis. III. The mecha- nism of bacterial trapping by polymerizing fbrin. Surgery 1982;92:513-9
  4. Fukatsu K, Saito H, Han I, Yasuhara H, Lin MT, Inoue T, et al. The greater omentum is the primary site of neutrophil exudation in peritonitis. J Am Coll Surg 1996;18:450-6
  5. Cranshaw ML, Leak LV. Milky spots of the omentum: a source of peritoneal cells in the normal and stimulated ani- mal. Arch Histol Cytol 1990;53 Suppl:165-77
  6. Solvason N, Kearney JF. The human fetal omentum: a site of B cell generation. J Exp Med 1992;175:397-404
  7. Wijffels JF, Hendrickx RJ, Steenbergen JJ, Eestermans IL, Beelen RH. Milky spots in the mouse omentum may play an important role in the origin of peritoneal macrophages. Res Immunol 1992;143:401-9
  8. Topley N, Mackenzie RK, Williams JD. Macrophages and mesothelial cells in bacterial peritonitis. Immunobiology 1996;195:563-73
  9. Agca B, Paksoy M, Polat E, Aksin E, Dirican A, Durgun V, et al. Infuence of omentectomy on peritoneal defense mecha- nisms in an experimental model of intra-abdominal infection. Eur Surg Res 2003;35:35-40
  10. Weese JL, Ottery FD, Emoto SE. Does omentectomy prevent malignant small bowel obstruction? Clin Exp Metastasis 1988;6:319-24
  11. De Bakker JM, De Wit AW, Koerten HK, Ginsel LA, Daems WT. On the origin of peritoneal resident macrophages. III. EM-immunocytochemical studies on the origin of mouse peritoneal resident macrophages. J Submicrosc Cytol 1985;17:153-9
  12. Dux K. Proliferative activity of macrophages in the great- er omentum of the mouse in relation to the early postna- tal development of the vascular structures. J Leukoc Biol 1986;40:445-58
  13. Ambroze WL Jr, Wolff BG, Kelly KA, Beart RW Jr, Dozois RR, Ilstrup DM. Let sleeping dogs lie: role of the omentum in the ileal pouch-anal anastomosis procedure. Dis Colon Rectum 1991;34:563-5
  14. Shimotsuma M, Takahashi T, Kawata M, Dux K. Cellular subsets of the milky spots in the human greater omentum. Cell Tissue Res 1991;264:599-601
  15. Agalar F, Sayek I, Cakmakçi M, Hasçelik G, Abbasoglu O. Effect of omentectomy on per