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Journals / Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi / 2020 / Cilt: 6 - Sayı: 3

Determination of Optimum Ethanolic Extraction Conditions and Phenolic Profiles of Thyme, Mint, Uckun, Grape Seeds and Green Tea Waste Fiber

Kekik, Nane, Uçkun, Üzüm Çekirdeği ve Yeşil Çay Çöpü Lifinde Optimum Etanolik Ekstraksiyon Şartlarının ve Fenolik Profillerinin Belirlenmesi

Menekşe BULU
Hacer AKPOLAT
Yusuf TUNÇTÜRK
Duried ALWAZEER
Ayşe TÜRKHAN
Pages
605–614
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

HPLC analysis was performed for the extracts showing highest antioxidant activity, and green tea waste fiber (699.89 mg 100 g-1 dw) had the highest concentration of phenolic compounds overall, while mint (173.67 mg 100 g-1 dw) had the lowest amount.

📌 3 Key Takeaways

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Objective & Hypothesis:The objective of this study was to investigate the effect of different ethanol ratios in extraction solvent as well as the antioxidant properties of five plants.
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Key Findings & Data:Individual phenolic components were analyzed with reverse phase high performance liquid chromatography (HPLC).
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Conclusion & Impact:HPLC analysis was performed for the extracts showing highest antioxidant activity, and green tea waste fiber (699.89 mg 100 g-1 dw) had the highest concentration of phenolic compounds overall, while mint (173.67 mg 100 g-1 dw) had the lowest amount.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:antioksidan · antioksidan aktivite · fenolik bileşenler

Abstract

The objective of this study was to investigate the effect of different ethanol ratios in extraction solvent as well as the antioxidant properties of five plants. Thyme, mint, uckun, grape seeds and green tea waste fiber was analyzed to determine total phenolic content (TPC) and antioxidant activity by ABTS and DPPH radical scavenging activity assays. Individual phenolic components were analyzed with reverse phase high performance liquid chromatography (HPLC). TPC varied significantly from 2.00±0.27 to 172.68±0.19 mg GAE g-1 dw depending on the plant type and ethanol ratio of the solvent. The effect of ethanol ratio also varied among different plants. HPLC analysis was performed for the extracts showing highest antioxidant activity, and green tea waste fiber (699.89 mg 100 g-1 dw) had the highest concentration of phenolic compounds overall, while mint (173.67 mg 100 g-1 dw) had the lowest amount. Correlations between TPC and antioxidant activity was significant which is comparable to the previous report.

Özet

Bu çalışmanın amacı beş bitkinin antioksidan özelliklerini ve farklı etanol oranlarının ektraksiyona etkisini belirlemektir. Kekik, nane, uçkun, üzüm çekirdeği ve yeşil çay çöpü lifinin toplam fenolik madde içeriği (TPC) ve ABTS ve DPPH radikal giderme aktivitesi testleri kullanılarak antioksidan aktiviteleri ölçülmüştür. Bireysel fenolik maddeler ters faz yüksek performanslı sıvı kromotografisi (HPLC) kullanılarak analiz edildi. TPC bitki türü ve çözücü etanol oranına bağlı olarak 2.00±0.27 ile 172.68±0.19 mg GAE g-1 dw arasında değişti. Etanol oranının etkisi farklı bitkiler için değişiklik gösterdi. En yüksek antioksidan aktiviteye sahip ekstraktların HPLC analizleri en yüksek fenolik bileşen konsantrasyonu yeşil çay çöpü lifinde (699.89 mg 100 g-1 dw), en düşük ise nanede (173.67 g 100 g-1 dw) bulundu. Toplam fenolik madde içeriği ve antioksidan aktivite arasında daha önceki çalışmalarda da gösterildiği gibi kayda değer bir korelasyon belirlendi.

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Cite This Article

BULU, M., AKPOLAT, H., TUNÇTÜRK, Y., ALWAZEER, D., TÜRKHAN, A. (2020). Determination of Optimum Ethanolic Extraction Conditions and Phenolic Profiles of Thyme, Mint, Uckun, Grape Seeds and Green Tea Waste Fiber. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, Cilt: 6 - Sayı: 3, 605–614. https://ajindex.com/articles/321656

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References (11)

  1. Altmann, H. J., Grunow, W., Mohr, U., Richter-Reichhelm, H.B., & Wester, P.W. (1986). Effects of BHA and related phenols on the forestomach of rats. Food and Chemical Toxicology, 24, 1183–1188.
  2. Arnao, M. B. (2001). Some methodological problems in the determination of antioxidant activity using chromogen radicals: A practical case. Trends in Food Science and Technology, 11, 419–421.
  3. Alwazeer, D., & Dham, S. (2018). Presumptive Relationship between Oxidoreduction Potential and Both Antibacterial and Antioxidant Activities of Herbs and Spices: Oxidoreduction Potential as a Companion Tool for Measuring the Antioxidant Activity. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47, 506–514.
  4. Bahadori, M. B. (2018). Phenolic composition and functional properties of wild mint (Mentha longifolia var. calliantha (Stapf) Briq.). International Journal of Food Properties, 21, 183-193. Barizão, É. O., de Cinque Almeida, V., de Silva, B. C., Visentainer, J. V., Montanher, P. F., & Boeing, J. S. (2014). Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis. Chemistry Central Journal, 8, 1–9.
  5. Beato, V. M., Orgaz, F., Mansilla, F., & Montañon, A. (2011). Changes in Phenolic Compounds in Garlic (Allium sativum L.) owing to the cultivar and location of growth. Plant Foods for Human Nutrition, 66, 218–223.
  6. Blois, M. S. (1958) Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181, 1199–1200.
  7. Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a Free Radical Method to Evaluate Antioxidant Activity. Lebensmittel-Wissenschaft & Technologie, 28, 25–30.
  8. Brito, A., Ramirez, J. E., Areche, C., Sepúlveda, B., & Simirgiotis, M. J. (2014). HPLC-UV-MS profiles of phenolic compounds and antioxidant activity of fruits from three citrus species consumed in Northern Chile. Molecules, 19, 17400–17421.
  9. Dalia, Y., & El-Adawi, H. (2006). Study on grape seeds extraction and optimization: An approach. Journal of Applied Sciences, 6, 2944-2947.
  10. Dai, J., & Mumper, R. J. (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 15, 7313–7352.
  11. Fatiha, B., Didier, H., Naima, G., Khodir, M., Martin, K., Léocadie, K., Caroline, S., Mohamed, C., & Pierre, D. (2015). Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M