Journals / Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2016 / Cilt: 20 - Sayı: 3

Gerberanın (Gerbera jamesonii Bolus) Farklı Renklerde Işık Yayan LED Lambalar ile Aydınlatılmış Koşullarda Mikroçoğaltımı

Micropropagation of Gerbera (Gerbera jamesonii Bolus) under Different Color of Light- Emitting Diodes

Pages
468–474
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Rooted plants were successfully acclimatized to outdoor conditions with 84% survival rate in peat + perlite mixture (2:1)

📌 3 Key Takeaways

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Objective & Hypothesis:In present research, the effects of light quality on micropropagation of gerbera were investigated.
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Key Findings & Data:The MS medium containing 1 mg L-1 BAP and 0.1 mg L-1NAA was used in proliferation stage and the MS medium containing 0.05 mg L-1IBA was used in rooting stage of the study.
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Conclusion & Impact:Rooted plants were successfully acclimatized to outdoor conditions with 84% survival rate in peat + perlite mixture (2:1)
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In present research, the effects of light quality on micropropagation of gerbera were investigated. The MS medium containing 1 mg L-1 BAP and 0.1 mg L-1NAA was used in proliferation stage and the MS medium containing 0.05 mg L-1IBA was used in rooting stage of the study. Cultured plants were grown in cabinets illuminated with red, blue, daylight LED lamps separately and their various mixtures. Cool white fluorescent lamp was used as control treatment in the study. The highest ratio of explant with new shoots, the number of shoots per explant and shoot length values were obtained from cool white fluorescent lamps (90%, 5.35 shoots and 2.63 cm, respectivey), 100% blue LED lamp (95%, 5.24 shoots and 2.40 cm, respectively), 100% cool white LED lamp (90%, 4.18 shoots and 2.21 cm, respectively) and 70% red + 30% blue LED lamp treatments (80%, 4.14 shoots and 2.21cm, respectively) in the proliferation stage of the study. These treatments also yielded the highest values in terms of plant fresh and dry weight. While the highest rooting percentage (75%) was obtained from 100% cool white LED light treatment, the differences between the treatments except for 100% red LED light were not significant. Rooted plants were successfully acclimatized to outdoor conditions with 84% survival rate in peat + perlite mixture (2:1)

Özet

Bu çalışmada gerberanın mikroçoğaltımı üzerine ışık kalitesinin etkileri araştırılmıştır. Araştırmanın çoğaltma aşamasında 1 mg L-1 BAP ve 0.1 mg L-1 NAA, köklendirme aşamasında ise 0.05 mg L-1 IBA içeren MS ortamı kullanılmıştır. Kültüre alınan bitkiler; kırmızı, mavi ve gün ışığı yayan LED lambaların yalın ve değişik oranlarda karışımları ile aydınlatılmış kabinlerde geliştirilmiştir. Beyaz fluoresan lamba kontrol olarak kullanılmıştır. Çoğaltma aşamasında; en yüksek yeni sürgün oluşturan eksplant oranı, sürgün sayısı ve sürgün uzunluğu değerleri beyaz fluoresan lamba (sırasıyla, %90, 5.35 adet ve 2.63 cm), %100 mavi LED lamba (sırasıyla, %95, 5.24 adet ve 2.40 cm), %100 soğuk beyaz LED lamba (sırasıyla, %90, 4.18 adet ve 2.21 cm) ve %70 kırmızı + %30 mavi LED lamba (sırasıyla, %80, 4.14 adet ve 2.21 cm) uygulamalarından elde edilmiştir. Bu uygulamalar, bitki yaş ve kuru ağırlıkları bakımından da en yüksek değerleri vermiştir. Çalışmada, %100 kırmızı LED lamba uygulamasının köklenmeyi azalttığı belirlenmiştir. Köklenmiş bitkiler torf+perlit (2:1) karışımı içeren saksılara şaşırtılarak %84 oranında dış koşullara alıştırılmıştır

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Cite This Article

GÖK, K. M., ŞAN, B., BAYHAN, A. K. (2016). Gerberanın (Gerbera jamesonii Bolus) Farklı Renklerde Işık Yayan LED Lambalar ile Aydınlatılmış Koşullarda Mikroçoğaltımı. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 20 - Sayı: 3, 468–474. https://ajindex.com/articles/310583

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