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Journals / Romatizma Dergisi / 2007 / Cilt: 22 - Sayı: 2

Osteoartrit hastalarında DNA hasarı ve oksidatif stres: Pilot çalışma

DNA damage and oxidative stress in patients with osteoarthritis: a pilot study

Özlem ALTINDAĞ
Abdurrahman KOÇYİĞİT
Necla ÇELİK
HAKİM ÇELİK
Neslihan SORAN
Pages
60–63
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: The findings indicated that Reactive Oxygen Species produced in the progression of the disease may induce oxidative damage in normal cells

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: We aimed to investigate the effect of osteoarthritis on basal levels of DNA strand breaks and on the oxidative/anti-oxidative status of patients with osteoarthritis, and healthy subjects.
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Key Findings & Data:Patients and Methods: The study population contained 29 patients with knee osteoarthritis and 26 healthy controls.
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Conclusion & Impact:Conclusion: The findings indicated that Reactive Oxygen Species produced in the progression of the disease may induce oxidative damage in normal cells
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Tek-hücre analizi · DNA hasarı · Osteoartrit

Abstract

Objective: We aimed to investigate the effect of osteoarthritis on basal levels of DNA strand breaks and on the oxidative/anti-oxidative status of patients with osteoarthritis, and healthy subjects. Patients and Methods: The study population contained 29 patients with knee osteoarthritis and 26 healthy controls. We used single-cell gel electrophoresis (also called comet assay) to measure DNA strand breaks in peripheral blood mononuclear leukocytes. Plasma levels of total antioxidant status (TAS) and total oxidative status (TOS) were determined, and oxidative stress index OSI was calculated using a novel automated measurement method. Results: The mean values of DNA damage, plasma TOS and OSI were significantly higher in patients with osteoarthritis than in controls (p < 0.001, for all). Plasma TAS level was lower in patients than in healthy controls (p < 0.001). There was a significantly positive correlation between OSI and DNA damage (r = 0.530, p < 0.001). Conclusion: The findings indicated that Reactive Oxygen Species produced in the progression of the disease may induce oxidative damage in normal cells

Özet

Amaç: Bu çalışma osteoartritli hastalarda DNA hasarı ile serum oksidatif/ antioksidatif durumun araştırılması amacıyla planlandı. Hastalar ve Yöntem: Çalışmaya diz osteoartriti olan 29 hasta ve 26 sağlıklı kontrol grubu dahil edildi. Periferik lökosit DNA hasarı single-cell jel elektroforez (comet) yöntemi ile, plazma total oksidan, antioksidan miktarı ve oksidatif stres indeksi novel otomatik ölçüm yöntemi ile değerlendirildi. Bulgular: Periferik lökosit DNA hasarı, plazma total oksidan (TOS) miktarı ve oksidatif stres indeksi (OSI) osteoartrit grubunda kontrol grubuna göre anlamlı ölçüde yüksek, total antioksidan miktarı ise düşük bulundu (p < 0.001). Ayrıca DNA hasarı ile oksidatif stres indeksi arasında pozitif korelasyon mevcuttu (r = 0.530, p < 0.001). Sonuç: Çalışma sonuçlarımıza göre osteoartrit patojenezinde oksidatif stresin rolü olabileceğini ve buna bağlı gelişebilecek DNA hasarının osteoartritli hastalarda normal populasyona göre daha fazla olduğu düşünülebilir.

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Cite This Article

ALTINDAĞ, Ö., KOÇYİĞİT, A., ÇELİK, N., ÇELİK, H., SORAN, N. (2007). Osteoartrit hastalarında DNA hasarı ve oksidatif stres: Pilot çalışma. Romatizma Dergisi, Cilt: 22 - Sayı: 2, 60–63. https://ajindex.com/articles/305970

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