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Journals / Mikrobiyoloji Bülteni / 2004 / Cilt: 38 - Sayı: 4

Evaluation of the tween 80 opacity test for detection of the lipolytic activity of various Candida species and its utility in differentiation of C. albicans and C. dubliniensis

Çeşitli Candida türlerinin lipolitik aktivitelerinin saptanmasında tween 80 opaklaşma testinin değerlendirilmesi ve C. albicans ile C. dubliniesis ayırımındaki yeri

GÜLLÜ İŞTAR DOLAPÇI
FAZIL ALPER TEKELİ
SEVTAP ARIKAN AKDAĞLI
Pages
429–433
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Tween 80 medium may be useful for the presumptive identification of some Candida species but regarding our results further large scale studies are necessary to establish the role of the medium in differentiating particularly the two similar species, C.albicans and C.dubliniensis.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study a total of 253 strains belonging to 10 different species of Candida were examined for their lipolytic activity by using the Tween-80 opacity test.
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Key Findings & Data:Hundred and seventy-four of 182 C.albicans, all of 21 C.tropicalis, 1 C.rugosa and 2 of 5 C.famata strains produced halo on Tween 80 medium and around the inoculum after 10 days of incubation pointing out the positive lipolytic (esterase) activity.
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Conclusion & Impact:Tween 80 medium may be useful for the presumptive identification of some Candida species but regarding our results further large scale studies are necessary to establish the role of the medium in differentiating particularly the two similar species, C.albicans and C.dubliniensis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Candida · Candida albicans · Esterazlar

Abstract

In this study a total of 253 strains belonging to 10 different species of Candida were examined for their lipolytic activity by using the Tween-80 opacity test. Hundred and seventy-four of 182 C.albicans, all of 21 C.tropicalis, 1 C.rugosa and 2 of 5 C.famata strains produced halo on Tween 80 medium and around the inoculum after 10 days of incubation pointing out the positive lipolytic (esterase) activity. Seven of 8 negative C.albicans strains were isolated from hemocultures of patients with different malignancies. Remaining strains of Candida species; C.glabrata (12), C.dubliniensis (6), C.kefyr (8), C.guillermondii (5) and C.spherica (3) did not produce the halo. Tween 80 medium may be useful for the presumptive identification of some Candida species but regarding our results further large scale studies are necessary to establish the role of the medium in differentiating particularly the two similar species, C.albicans and C.dubliniensis.

Özet

Bu çalışmada, 10 farklı Candida türünden oluşan toplam 253 susun tween 80 opaklaşma testi kullanılarak, lipolitik aktiviteleri değerlendirilmiştir. Tween 80 besiyerinde 10 günlük izlem sonucunda inokulümün etrafında halo izlenmesi pozitif lipolitik (esteraz) aktivitesi olarak kabul edilmiş ve 182 C.albicans susunun 174'ünün, 21 C.tropicalis susunun tamamının, 1 C.rugosa susu ve 5 C.famata susunun 2'sinin halo oluşturduğu izlenmiştir. Negatif sonuç veren 8 C.albicans susunun 7'sinin çeşitli maligniteleri olan hastaların kan kültürlerinden izole edilen suşlar olduğu görülmüştür. Geri kalan diğer Candida türlerine ait suşlardan; 12 C.glabrata, 6 C.dubliniensis, 8 C.kefyr, 5 C.guillermondii ve 5 C.spherica susunda halo oluşumu izlenmemiştir. Tween 80 besiyerinin bazı Candida türlerinin ön tanısında faydalı olabileceği ancak özellikle çok benzer iki tür olan C.albicans ve C.dubliniensis'in ayırımı için daha detaylı çalışmalara ihtiyaç olduğu düşünülmüştür.

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Cite This Article

DOLAPÇI, G. İ., TEKELİ, F. A., AKDAĞLI, S. A. (2004). Evaluation of the tween 80 opacity test for detection of the lipolytic activity of various Candida species and its utility in differentiation of C. albicans and C. dubliniensis. Mikrobiyoloji Bülteni, Cilt: 38 - Sayı: 4, 429–433. https://ajindex.com/articles/299660

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