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Journals / Mikrobiyoloji Bülteni / 2003 / Cilt: 37 - Sayı: 1

In-vitro activity of antimicrobial agents against Haemophilus influenzae strains isolated from various clinical specimens

Klinik örneklerden izole edilen Haemophilus influenzae suşlarının çeşitli antimikrobik ilaçlara in-vitro duyarlılığı

Fatma BUDAK
Deniz GÜR
Pages
19–25
DOI
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⚡ TL;DR(Too Long; Didn't Read)

According to these results, resistance to ampicillin is low in our H.influenzae isolates compared to otherEuropean countries but resistance to TMP/SMX is very high and this finding has to be taken into account in the empirical therapy of community acguired respiratory tract infections.

📌 3 Key Takeaways

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Objective & Hypothesis:In-vitro activity of several antimicrobiâl agents were evaluated against Haemophilus influenzae (n: 127) isolated from clinical specimens vvithin the years 2000 and 2002 in a children's hospital.
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Key Findings & Data:Resistance rates for the antibiotics were as follows: ampicillin 5.6%, trimethoprim-sulfamethoxazole (TMP/SMX) 27.5%, tetracycline 8.6%, clarithromycin 7%, chloramphenicol 4.7%.
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Conclusion & Impact:According to these results, resistance to ampicillin is low in our H.influenzae isolates compared to otherEuropean countries but resistance to TMP/SMX is very high and this finding has to be taken into account in the empirical therapy of community acguired respiratory tract infections.
🔬Design:Research Article
📍Scope:2000 Verileri
⚙️Variables:Mikrobiyal duyarlılık testleri · beta-Laktamazlar · İlaç direnci

Abstract

In-vitro activity of several antimicrobiâl agents were evaluated against Haemophilus influenzae (n: 127) isolated from clinical specimens vvithin the years 2000 and 2002 in a children's hospital. Antimicrobiâl susceptibility tests were performed by disk diffusion method according to the recommendations of the NCCLS standards and beta-lactamase activity was investigated by nitrocefin test. Resistance rates for the antibiotics were as follows: ampicillin 5.6%, trimethoprim-sulfamethoxazole (TMP/SMX) 27.5%, tetracycline 8.6%, clarithromycin 7%, chloramphenicol 4.7%. Five isolates (3.9%) were found multiple resistant. Beta-lactamase activities were detected in ali of the ampicillin resistant isolates. None of the isolates were resistant to ampicillin-sulbactam, cefaclor, cefuroxime, cefprozil, cefpodoksim, cefotaxime, meropenem ör ciprofloxacin. According to these results, resistance to ampicillin is low in our H.influenzae isolates compared to otherEuropean countries but resistance to TMP/SMX is very high and this finding has to be taken into account in the empirical therapy of community acguired respiratory tract infections.

Özet

Hacettepe Üniversitesi Çocuk Hastanesi Klinik Mikrobiyoloji Laboratuvarına 2000-2002 yılları arasında gelen klinik örneklerden izole edilen 127 Haemophilus influenzae susunun 13 antimikrobik ilaca in-vitro duyarlılığı NCCLS standartlarına uyularak disk difüzyon yöntemi ile araştırılmış, beta-laktamaz aktivitesi nitrosefin yöntemi ile belirlenmiştir. Ampisiline %5.6, trimetoprim sulfametoksazole (TMP/SMX) %27.5, tetrasikline %8.6, klaritromisine %7, kloramfenikole %4.7 oranında direnç gözlenirken, suşlarm hiç birinde ampisilin-sulbaktam, sefaklor, sefuroksim, sefprozil, sefpodoksim, sefotaksim, meropenem ve siprofloksasine direnç saptanmamıştır. Ampisiline dirençli tüm suşlarda beta-laktamaz aktivitesi gösterilmiştir. Beş izolatta (%3.9) çoğul direnç saptanmıştır. Bu sonuçlar, toplum kökenli H.influenzae izolatlarında ampisiline direncin'diğer Avrupa ülkelerine kıyasla daha düşük olduğunu, ancak TMP/SMK ve tetrasikline karşı direncin yüksek olduğunu göstermektedir.

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Cite This Article

BUDAK, F., GÜR, D. (2003). In-vitro activity of antimicrobial agents against Haemophilus influenzae strains isolated from various clinical specimens. Mikrobiyoloji Bülteni, Cilt: 37 - Sayı: 1, 19–25. https://ajindex.com/articles/299441

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