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Journals / Mikrobiyoloji Bülteni / 2004 / Cilt: 38 - Sayı: 1

Esterase activities of Candida species

Candida türlerinde esteraz aktivitesi

SEMA AŞKIN KEÇELİ
Fatma BUDAK
Pages
99–103
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our results showed that, 83 of 86 (96.5%) Candida albicans, and all of the C. tropicalis and C. guilliermondii strains (100%) yielded a halo around their colonies.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to determine the esterase activity of clinically important Candida species and to evaluate tween 80 opacity test medium as an additional identification method.
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Key Findings & Data:A total of 118 Candida isolates (86 C. albicans, 12 C. tropicalis, 5 C. glabrata, 4 C. krusei, 3 C. guilliermondii, 3 C. kefyr, 3 C. parapsilosis, 1 C. famata, 1 C. dubliniensis) were examined for their response to tween 80 opacity test.
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Conclusion & Impact:Our results showed that, 83 of 86 (96.5%) Candida albicans, and all of the C. tropicalis and C. guilliermondii strains (100%) yielded a halo around their colonies.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Esterazlar · Kültür ortamı · Candida

Abstract

The aim of this study was to determine the esterase activity of clinically important Candida species and to evaluate tween 80 opacity test medium as an additional identification method. A total of 118 Candida isolates (86 C. albicans, 12 C. tropicalis, 5 C. glabrata, 4 C. krusei, 3 C. guilliermondii, 3 C. kefyr, 3 C. parapsilosis, 1 C. famata, 1 C. dubliniensis) were examined for their response to tween 80 opacity test. Our results showed that, 83 of 86 (96.5%) Candida albicans, and all of the C. tropicalis and C. guilliermondii strains (100%) yielded a halo around their colonies. The remaining Candida species did not produce a positive test response after 13 days of incubation. It can be concluded that, tween 80 opacity test is a simple and economical test that can be used as a supplementary test for the identification of Candida species.

Özet

Bu çalışmanın amacı, klinik açıdan önemli Candida türlerinin esteraz aktivitesini belirlemek ve bu amaçla tween 80 opasite testinin bir tanımlama metodu olarak kullanılabilirliğini değerlendirmektir. Çalışmada, 118 Candida susu (86 C.albicans, 12 C.tropicalis, 5 C.glabrata, 4 C.krusei, 3 C.guilliermondii, 3 C.kefyr, 3 C.parapsilosis, 1 C.famata, 1 C.dubliniensis) tween 80 opasite testine yanıtı açısından incelenmiştir. C.albicans suşlarının 83'ünde (%96.5), C.tropicalis ve C.guilliermondii suşlarının ise tamamında (%100) inokülasyon bölgesinin etrafında hale oluşumu saptanmıştır. Diğer suşlarda inokülasyondan 13. günden sonra dahi esteraz aktivitesi belirlenmemiştir. Sonuç olarak, basit ve ekonomik olan tween 80 opasite testinin Candida türlerinin ayırımında ek bir tanımlama yöntemi olarak kullanılabileceği düşünülmüştür.

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Cite This Article

KEÇELİ, S. A., BUDAK, F. (2004). Esterase activities of Candida species. Mikrobiyoloji Bülteni, Cilt: 38 - Sayı: 1, 99–103. https://ajindex.com/articles/299418

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