The comparison of DNA extraction techniques in human bone and tooth samples exposed to high heat

In the study, present extraction methods are compared and as a result it was suggested that phenol-chloroform and Qiagen DNA Mini Kit could be used in routine with a newly developed modified procedure in order to increase efficiency and efficiency in forensic genetic identification.

📌 3 Key Takeaways

🎯

Objective & Hypothesis:In the identification of forensic cases, it is critical to obtain information to identify the victim’s identity from burnt bone and tooth remains.

📊

Key Findings & Data:When bone and tooth are burned, both their physical and chemical properties change significantly, which prevents anthropological evaluations and successful DNA profiling for identification purposes.

💡

Conclusion & Impact:In the study, present extraction methods are compared and as a result it was suggested that phenol-chloroform and Qiagen DNA Mini Kit could be used in routine with a newly developed modified procedure in order to increase efficiency and efficiency in forensic genetic identification.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Özet

In the identification of forensic cases, it is critical to obtain information to identify the victim’s identity from burnt bone and tooth remains. When bone and tooth are burned, both their physical and chemical properties change significantly, which prevents anthropological evaluations and successful DNA profiling for identification purposes. Heat causes difficulties in the identification techniques in which bones and teeth are used, depending on the degree of heat exposure. For this reason, with the changes occurring in bone and tooth during combustion, it is necessary to evaluate the results of observation and analysis, and to be used the information obtained in a certain order. It is very difficult to extract DNA from the samples obtained from fire, explosion, motor vehicle and aircraft accidents and other traumatic events by traditional methods. The only biological material available to determine the typing and the identification of missing persons or unknown remains in different situations, such as mass fatality events, wars or socio-political events, is often only human remains. Effective DNA extraction procedures are critical steps in successful DNA analysis of skeletal remains. Due to variations in DNA and heterogeneity within the bone, unfortunately, the only reliable method for obtaining DNA from overly degraded specimens such as burnt bones and teeth in the literature, is still not available. In the study, present extraction methods are compared and as a result it was suggested that phenol-chloroform and Qiagen DNA Mini Kit could be used in routine with a newly developed modified procedure in order to increase efficiency and efficiency in forensic genetic identification.

🔖

Cite This Article

YUKSELOGLU, E. H., DASTAN, K., YONAR, F. C., RAYİMOĞLU, G., KARATAŞ, Ö., ISLEK, D. S., DOGAN, M. B. (2019). The comparison of DNA extraction techniques in human bone and tooth samples exposed to high heat. Medicine Science, Cilt: 8 - Sayı: 3, 489–495. https://ajindex.com/articles/277852

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (10)

Gustavson G. Dental Identification, Forensic odontology. London; Staples Press; 1996. Yaşar F, Hancı Hİ, Afşin H. Dişlerin ı̇ncelenmesinin adli yönden önemi. Adli Tıp ve Adli Bilimler. Ankara, Seçkin Yayınevi, 2002;213-31. Afşin H, Günce EM. Adli diş hekimliği açısından olay yeri incelemesi. Adli Tıp Dergisi. 2002;24:94-106.
Mayal SS, Agarwal P, Vashist P. Dental DNA finger-printing in identification of human remains. Ann Dental Specialty. 2013;01:01. Datta P, Sood S, Rastogi P, et al. DNA profiling in forensic dentistry. J Indian Acad Forensic Med. 2012;34:2. Tuğ A, Yaşar F. Felaket kurbanlarının kimliklendirilmesi çalışmalarında diş hekimlerinin ve diş incelemelerinin önemi. Hacettepe Diş Hekimliği Fakültesi Dergisi. 2006;30:77-82.
Ricaut FX, Tracqui CK, Crubezy E, et al. STR-Genotyping from human medieval tooth and bone samples. Forensic Sci Int. 2005;151:31-5.
Rohland N, Hofreiter M. Ancient DNA extraction from bones and teeth. Nat Protoc. 2007;2:1756-62.
Silva RHA, Quiezi R, Bertolacini CDP, et al. Human identification analysis using pcr from the root portion of dental elements under different conditions of temparature and exposure time. RSBO. 2012;9:67-73.
Sajantila A, Stro ̈m M, Budowle B, et al. The polymerase chain reaction and post-mortem forensic identity testing: application of amplified D1S80 and HLA-DQa loci to the identification of fire victims. Forensic Sci Int. 1991;51:23–34.
Adler CJ, Haak W, Donlon D, et al. Survival and recovery of DNA from ancient teeth and bones. J Archeol Sci. 2011;38:956-64.
Hill AJ, Lain R, Hewson I. Preservation of dental evidence following exposure to high temperature. Forensic Sci Int. 2010;6185:4. Schwark T, Heinrich A, Preuße-Prange A, et al. Reliable genetic identification of burnt human remains. Forensic Sci Int Genet. 2011;5:393-9. Pa ̈a ̈bo S, Poinar H, Serre D, et al. Genetic analyzes from ancient DNA. Annu Rev Genet. 2004;38:645-79.
Issaquah WA, Bohnert M, Rost T, Pollak S. The degree of destruction of human bodies in relation to the duration of the fire. Forensic Sci Int. 1998;95:11-21.
Icove DJ, DeHaan JD. Forensic Fire Scene Reconstruction-Brady Fire. Upper Saddle River, NJ; Prentice Hall; 2009. DeHaan JD. Repositioning of human bodies during fires. In: Proceedings of ınternational symposium on fire ınvestigation science and technology. Sarasota, FL; 2012. Christensen AM. Experiments in the combustibility of the human body. J Forensic Sci. 2002;47:466-70. Drysdale DD. An Introduction to