Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2010 / Cilt: 16 - Sayı: 4

Isolation of Arcanobacterium pyogenes from samples of sheep and cattle and identification by Polimerase Chain Reaction

Koyun ve sığır örneklerinden Arcanobacterium pyogenes izolasyonu ve Polimeraz zincir reaksiyonu ile identifikasyonu

Pages
611–616
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

There is emphasized that this bacterium may cause a variety of infections with economically losses in sheep and cattle.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aims of this study were to isolate Arcanobaterium pyogenes (A. pyogenes) from cattle and sheep, to identify the isolates by conventional methods, Polymerase Chain Reaction (PCR) and to determine antimicrobial susceptibilities.
📊
Key Findings & Data:In this study, a total 716 samples was collected from sheep and cattle (liver, milk, broncho alveoler lavage, lung, suppurative tissue, and fluid of joint).
💡
Conclusion & Impact:There is emphasized that this bacterium may cause a variety of infections with economically losses in sheep and cattle.
🔬Design:Research Article
📍Scope:716 samples
⚙️Variables:izolasyon · koyun · Arcanobacterium pyogenes

Abstract

The aims of this study were to isolate Arcanobaterium pyogenes (A. pyogenes) from cattle and sheep, to identify the isolates by conventional methods, Polymerase Chain Reaction (PCR) and to determine antimicrobial susceptibilities. In this study, a total 716 samples was collected from sheep and cattle (liver, milk, broncho alveoler lavage, lung, suppurative tissue, and fluid of joint). All samples were cultured onto blood agar base containing 5% defibrinated sheep blood and plates were incubated at 37ºC for 48 h. Total 51 A. pyogenes strains isolated from samples were identified in according to properties of biochemical. Then, PCR analyses were made using specific primers for A. pyogenes. All isolates were also confirmed by PCR. In addition, antimicrobial activities were determined to 16 antimicrobial agents, some of which were used in veterinary medicine. There is emphasized that this bacterium may cause a variety of infections with economically losses in sheep and cattle.

Özet

Bu çalışmanın amacı; koyun ve sığır örneklerinden Arcanobacterium pyogenes (A. pyogenes) izole etmek, Polimeraz zincir reaksiyonu (PCR) ile identifiye etmek ve antimikrobiyal duyarlılıklarını belirlemektir. Çalışmada, koyun ve sığırlara ait (süt, akciğer, karaciğer, bronko alveolar yıkantı, eklem sıvısı ve süppuratif doku) toplam 716 adet örnek toplandı. Bütün örnekler, %5 koyun kanı içeren kanlı agara ekimleri yapıldı ve 37ºC’de 48 saat inkübe edildi. Örneklerden izole edilen toplam 51 adet A. pyogenes suşu biyokimyasal özelliklerine göre identifiye edildi. Daha sonra, A. pyogenes için plosin genine spesifik primerler kullanılarak PCR analizleri gerçekleştirildi. Bütün izolatlar PCR ile A. pyogenes olarak doğrulandı. Ayrıca, veteriner sahada kullanılan 16 farklı antimikrobiyal ajana karşı antimikrobiyal aktiviteleri belirlendi. Bu mikroorganizmanın, koyun ve sığırlarda ekonomik kayıplarla seyreden enfeksiyonlara sebep olabileceği vurgulandı.

🔖

Cite This Article

HADİMLİ, H., ERGANİŞ, O., KAV, K., SAYIN, Z. (2010). Isolation of Arcanobacterium pyogenes from samples of sheep and cattle and identification by Polimerase Chain Reaction. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 16 - Sayı: 4, 611–616. https://ajindex.com/articles/273826

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Narayanan S, Nagaraja TG, Staats J, Chengappa MM, Obersts RD: Biochemical and biological characterizations and ribotyping of Actynomyces pyogenes and Actinomyces pyogenes-like organisms from liver abscesses in cattle. Vet Microbiol, 61, 289-303, 1998
  2. Billington SJ, Songer JG, Jost BH: Molecular characterization of the pore-forming toxin, pyolosin, a major virulence determinant of Arcanobacterium pyogenes. Vet Microbiol, 82, 261-274, 2001
  3. Silva E, Gaiva M, Leita S, Jost BH, Carneiro C, Vilela CL, Lopes da Costa L, Mateus L: Genomic characterization of Arcanobacterium pyogenes isolates recovered from the uterus of dairy cows with normal puerperium or clinical metritis. Vet Microbiol, 25, 111-118, 2008
  4. Jost BH, Billington SJ: Arcanobacterium pyogenes: Molecular pathogenesis of an animal opportunist. Antonie van Leeuwenhoek, 88, 87-102, 2005
  5. Simpson RB, Wesen DP, Anderson KL, Armstrong JD, Harvey RW: Subclinical mastitis and milk production in primiparous simmental cows. J Anim Sci, 73, 1552-1558, 1995
  6. Jousimies-Somer H, Pyorala S, Kanervo A: Susceptibilities of bovine summer mastitis bacteria to antimicrobial agents. Antimicrob Agent Chemother, 40, 157-160, 1996
  7. Watts JL, Lowery DE, Teel JF, Rossbacht S: Identification of Corynebacterium bovis and Coryneforms isolated from bovine mammary glands. J Dairy Sci, 83, 273-2379, 2000
  8. Nolte O, Morscher J, Weiss HE, Sonntag HG: Auto- vaccination of dairy cows to treat post partum metritis caused by Actinomyces pyogenes. Vaccine 19, 3146-3153, 2001
  9. Quinn AK, Vermont JJ, Twiss DP: Arcanobacterium pyogenes mastitis in a 18-month-old heifer. New Zealand Vet J, 50, 167-168, 2002
  10. Gouletsou PG, Ethenakis GC, Cripps PJ, Papaioannou N, Lainas T, Psalla D, Amiridis GS: Experimentally induced orchitis associated with Arcanobacterium pyogenes: Clinical, ultrasonographic, seminological and pathological features. Theriogenol, 62, 1307-1328, 2004
  11. Gröhn YT, Wilson DJ, Gonzalez RN, Hertl JA, Schulte H, Bennett G, Schukken YH: Effect of pathogen-specific clinical mastitis on milk yield in dairy cows. J Dairy Sci, 87, 33583374, 2004
  12. Gouletsou PG, Ethenakis GC, Tzora A, Cripps PJ, Saratsis P: Isolation of Arcanobacterium pyogenes from the scrotal skin and the prepuce of healthy rams or from rams with testicular abnormalities. Small Rumin Res, 63, 177-182, 2006
  13. Miller ANA, Williams EJ, Sibley K, Herath S, Lane EA, Fishwick J, Nash DM, Rycroft AN, Dobson H, Bryant CE, Sheldon IM: The e