AI Summary & Key Takeaways
AI InsightAs a result, genotypic distributions were equilibrium in both pure and crossbred examples of Turkish Grey steppe cattle.
📌 3 Key Takeaways
Abstract
Calpastatin (CAST) is a specific inhibitor of calcium-dependent neutral protease μ-calpain found in mammalian tissues. The genetic variants in the bovine CAST gene were analysed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The animal material of the study consisted of 132 bulls and heifers in Turkish Grey steppe cattle and Turkish Grey steppe x Brown Swiss crossbreds. C and G alleles are characterized by CAST/RsaI polymorphism were detected in the sample of animals were studied. Allele frequency C was significantly frequent in the crossbred group compared to the pure Turkish Grey steppe animals (P<0.05). In the total samples of animals, the average allele frequency C was 56.1%. Genotypic frequencies were estimated as 0.257, 0.499 and 0.243 in the purebred Turkish Grey steppe, and 0.388, 0.470 and 0.142 in the Turkish Grey steppe x Brown Swiss crossbred population for genotypes CC, CG and GG, respectively. As a result, genotypic distributions were equilibrium in both pure and crossbred examples of Turkish Grey steppe cattle.
Özet
Kalpastatin (CAST), memeli dokularında bulunan ve kalsiyuma bağımlı doğal bir proteaz olan μ-kalpain’in spesifik bir inhibitörüdür. Sığır CAST geni içindeki genetik varyantlar, polimeraz zincir reaksiyonu-restriksiyon parça uzunluk polimorfizmi (PCR-RFLP) metodu kullanılarak analiz edildi. Çalışmada hayvan materyali olarak, Boz ırk ve Boz ırk x İsviçre Esmer melezlerinden oluşan 132 boğa ve düve kullanıldı. Çalışılan hayvan örneklerinde CAST/RsaI polimorfizminin C ve G alleleri karakterize edildi. C allel frekansı, saf Türk Boz ırklara nazaran melez grup içinde önemli bulunmuştur (P<0.05). Hayvan örneklerinin tümünde, C allel frekansı ortalaması %56.1’dir. Genotipik frekanslar, CC, CG ve GG genotipleri için sırasıyla, safkan Türk Boz ırkta; 0.257, 0.499 ve 0.243 ve Türk Boz ırk x İsviçre Esmer melezi popülasyonda; 0.388, 0.470 ve 0.142 olarak tahmin edildi. Sonuç olarak, genotipik dağılımlar Türk Boz ırk sığırı saf ve melez örneklerinin her ikisinde de dengededir.