Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2016 / Cilt: 22 - Sayı: 4

Tüketime Hazır Dönerlerde PCR ile Soya Proteininin Aranması

PCR Detection of Soy Protein in Ready to Eat Meat Doners

Pages
557–560
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The detection results of the doner samples showed that any of the 25 samples collected after the regulation enacted did not contain soy ingredient while 3 of 25 sample (12%) collected before the regulation came into force contained soy.

📌 3 Key Takeaways

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Objective & Hypothesis:Addition of soy protein sources in food products is widely used because of their functional properties such as water binding, fat binding, beneficial effects on texture and emulsification capability and providing improved economy with increasing yield.
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Key Findings & Data:Because of these reasons, the most recent meat products regulation of Turkish Food Codex has banned the addition of soy in doners since 2012, like several other countries.
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Conclusion & Impact:The detection results of the doner samples showed that any of the 25 samples collected after the regulation enacted did not contain soy ingredient while 3 of 25 sample (12%) collected before the regulation came into force contained soy.
🔬Design:Research Article
📍Scope:2012 · 25 samples
⚙️Variables:Domain Parameters

Abstract

Addition of soy protein sources in food products is widely used because of their functional properties such as water binding, fat binding, beneficial effects on texture and emulsification capability and providing improved economy with increasing yield. However, the use of soy protein in food products causes economical disadvantages because of replacement of an expensive ingredient like meat with a cheaper ingredient like soy and health risks for the consumers as well. Soy is an important allergy source for sensitive consumers. Because of these reasons, the most recent meat products regulation of Turkish Food Codex has banned the addition of soy in doners since 2012, like several other countries. Detection of soy in food products is performed by detection of soy protein or soy DNA. Because DNA is more stable to processing, PCR methods are shown to be more reliable when used in processed foods. In our study, 50 doner samples were collected from various retail sales points. Twenty-five doner samples were collected before and 25 after the regulation was enacted. DNA was isolated from doner samples and PCR testing of these DNA extracts were performed. The detection results of the doner samples showed that any of the 25 samples collected after the regulation enacted did not contain soy ingredient while 3 of 25 sample (12%) collected before the regulation came into force contained soy.

Özet

Soya proteinleri, gıda endüstrisinde, su bağlama, yağ bağlama, tekstür ve emülsifikasyon yeteneği, verim arttırma gibi fonksiyonel özelliklerinden ve verimin artışına bağlı olarak ekonomik karlılığı arttırmasından dolayı geniş kullanım alanı bulmaktadır. Ancak gıda ürünlerinde soya kullanımı, tüketiciler için ekonomik kayıplar ve sağlık riskleri gibi olumsuzluklara sebep olmaktadır. Soya, hassas tüketiciler için önemli bir alerji kaynağıdır. Bu gibi nedenlerden ülkemizde Aralık 2012'de çıkan Türk Gıda Kodeksi Et ve Et Ürünleri Tebliği ile dönerlerde soya kullanımı birçok diğer ülkelerde de olduğu gibi yasaklanmıştır. Gıdalarda soyanın tespit edilmesi soya proteinin ya da soya DNA'sının tespit edilmesi ile gerçekleştirilir. Ancak, DNA gıda işleme şartlara daha dayanıklı olması sebebiyle işlenmiş gıdalarda çok daha güvenilirdir. Çalışmamızda, 25 adedi Et ve Et Ürünleri Tebliğinin yürürlüğe girmesinden önce, diğer 25 adedinin ise tebliğin yürürlüğe girmesinden sonra olacak şekilde 50 adet döner örneği çeşitli perakende satış noktalarından toplanmıştır. Döner örneklerinden DNA izole edilmiş ve bu DNA'ların PZR testleri gerçekleştirilmiştir. Elde edilen sonuçlara göre Et ve Et Ürünleri Tebliğinin yürürlüğe girmesinden sonra toplanan hiçbir örnekte soya tespit edilemezken, tebliğin yayınlanmasından önce toplanan 25 örnekten 3'ünde (%12) soya varlığı saptanmıştır.

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Cite This Article

MURATOĞLU, K., EKER, F. Y., ARUN, Ö. Ö. (2016). Tüketime Hazır Dönerlerde PCR ile Soya Proteininin Aranması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 22 - Sayı: 4, 557–560. https://ajindex.com/articles/272502

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