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Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2018 / Cilt: 8 - Sayı: 3

Purification, Characterization of NADPH-Cytochrome P450 Reductase from Turkey Liver and Effects of Some Metal Ions on Enzyme Activity

Hindi Karaciğerinden NADPH-Sitokrom P450 Redüktaz’ın Saflaştırılması, Karakterizasyonu ve Bazı Metal İyonlarının Enzim Aktivitesi Üzerindeki Etkileri

Ebru AKKEMİK
Mehmet ÇİFTÇİ
Pages
183–189
Publication Date
2018-09-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It has been found that Ag+, Hg2+ and Cu2+ metal ions have an inhibitory effect on CPR enzymeactivity.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:NADPH-Cytochrome P450 reductase (CPR) is a key enzyme catalyzing electron transfer indetoxification metabolism.
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Key Findings & Data:In the first method, NADPH-Cytochrome P450 reductase was purified using 2ʹ, 5ʹ-ADP Sepharose 4Baffinity column with ~114 purification fold and ~23% yield.
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Conclusion & Impact:It has been found that Ag+, Hg2+ and Cu2+ metal ions have an inhibitory effect on CPR enzymeactivity.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Hindi karaciğeri · inhibisyon · karakterizasyon

Abstract

NADPH-Cytochrome P450 reductase (CPR) is a key enzyme catalyzing electron transfer indetoxification metabolism. In this study, two methods were employed to purify CPR enzyme from turkey livermicrosomes. In the first method, NADPH-Cytochrome P450 reductase was purified using 2ʹ, 5ʹ-ADP Sepharose 4Baffinity column with ~114 purification fold and ~23% yield. In the second method, CPR was purified using DE-52Cellulose anion exchange column and 2ʹ, 5ʹ-ADP Sepharose 4B affinity column with 124 purification fold and 8%yield. Enzyme purity was checked in both methods with SDS-PAGE. Characteristics kinetic features of the purifiedenzyme were identified. The effects of some metal ions on purified PCR enzyme activity have been investigatedin vitro conditions. It has been found that Ag+, Hg2+ and Cu2+ metal ions have an inhibitory effect on CPR enzymeactivity.

Özet

NADPH-Sitokrom P450 redüktaz (CPR) enzimi, detoksifikasyon metabolizmasında elektron transferini

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Cite This Article

AKKEMİK, E., ÇİFTÇİ, M. (2018). Purification, Characterization of NADPH-Cytochrome P450 Reductase from Turkey Liver and Effects of Some Metal Ions on Enzyme Activity. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 8 - Sayı: 3, 183–189. https://ajindex.com/articles/257115

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