Difficulties In Synthesis and Characterization of Viral Capsid Peptides

Key Findings & Data:In this work we synthesised 3 different viral peptide sequences with microwave enhanced Solid Phase Peptide Synthesis SPPS method and characerized with LC-ESI-MS system.

💡

Conclusion & Impact:We briefly describe the difficulties in synthesis and characterization.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Synthetic viral peptide · LC-ESI-MS analysis · Solid Phase Peptide Synthesis

Abstract

Peptides and proteins play a central role in numerous biological and physiological processes in living organisms. Viral capsid pepdides are part of the viruses outer shell of genetic materials. Viruses are recognized by immune system via capsid peptides. Depending on this property of capsid peptides, totally synthetic vaccine prototypes can be developed. In this work we synthesised 3 different viral peptide sequences with microwave enhanced Solid Phase Peptide Synthesis SPPS method and characerized with LC-ESI-MS system. We briefly describe the difficulties in synthesis and characterization.

Keywords

Synthetic viral peptide LC-ESI-MS analysis Solid Phase Peptide Synthesis

🔖

Cite This Article

ÖZDEMİR, Z. Ö., KARABULUT, E., TOPUZOĞULLARI, M., MUSTAFAEVA, Z. (2008). Difficulties In Synthesis and Characterization of Viral Capsid Peptides. Hacettepe Journal of Biology and Chemistry, Cilt: 36 - Sayı: 4, 329–337. https://ajindex.com/articles/256226

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (14)

Fischer, E. & Fourneau, E. (1901) Ueber einige Derivate des Glykokolls. Ber. Dtsch. Chem. Ges. 34, 2868–2879
Curtius, T. (1882) Ueber einige neue Hippursa¨ureanalog constituierte synthetisch dargestellte Aminosa¨uren. J. Prakt. Chemie 26, 145–208
Kimmerlin, T. & Seebach, D. ‘100 years of peptide synthesis’: ligation methods for peptide and protein synthesis with applications to bpeptide assemblies. J. Peptide Res., 2005, 65, 229–260
Merrifield, R. B. Solid Phase Synthesis. Nobel lecture, 8 December, 1984, The Rockefeller University, 1230 York Avenue, New York, N.Y. 10021-6399 5. Merrifield, R. B. (1963) Solid phase peptide synthesis. I. The synthesis of a tetrapeptide. J. Am. Chem. Soc. 85, 2149–2154
Carpino, L.A. (1957) Oxidative reactions of hydrazines. IV. Elimination of nitrogen from 1,1- disubstituted-2-arensulfonhydrazides. J. Am. Chem. Soc. 79, 4427–4430
McKay, F.C. & Albertson, N.F. (1957) New amine-masking groups for peptide synthesis. J. Am. Chem. Soc. 79, 4686–4690
Carpino, L.A. & Han, G.Y. (1970) The 9- fluorenylmethoxycarbonyl function, a new base-sensitive amino-protecting group. J. Am. Chem. Soc. 92, 5748–5749
Palese P (2006). “Making better influenza virus vaccines?”. Emerging Infect. Dis. 12 (1): 61–5
Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K., Watson, J. D., (1994). Molecular Biology of the Cell, 4, 280
Petrov R. V., Mustafaev, M. I., Norimov A., Physicochemical criteria for the construction of artificial immunomodulators and immunogens on the basis of polyelectrolyte complexes. In: Sov Med Rev D Immunol. UK: Harwood Academic Publishers GmbH; 1992. p. 1–113
Mustafaev M.,, “Functionally Biopolymer Systems”, Sigma Journal of Engineering and Natural Science, 4, 2004, pp. 1-201. 13. van Lierop, M. J. C., Wagenaar, J.A., van Noort, J.M., Hensen, E.J., Journal of Virology, 1995, 69, p. 4511–4514
Yedidia, T. B., Arnon, R., Synthetic peptidebased vaccines against influenza,1997, International Journal of Peptide Research and Therapeutics
Chapman, J.R. (2000) Mass Spectrometry of Proteins and Peptides. Sale, Manchester, UK
Chakraborty, A. B. and Berger, S. J., Optimization of Reversed-Phase Peptide Liquid Chromatography Ultraviolet Mass Spectrometry Analyses Using an Automated Blending Methodology, Journal of Biomolecular Techniques 16:325–333, 2005 ABRF 17. Aguilar, M.-I. (2004) HPLC of Peptides and Proteins: Methods and Protocols. Monash University, Clayton, Victoria, Aust