Journals / Hacettepe Journal of Biology and Chemistry / 2016 / Cilt: 44 - Sayı: 4

Antibiofilm Activity and Chemical Contents of Propolis Samples From Manisa-Turkey

Türkiye-Manisa’dan Propolis Örneklerinin Kimyasal İçeriği ve Antibiyofilm Aktivitesi

Pages
505–513
Publication Date
2016-11-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to GC/ MSD analyze, triacontyl acetate was the major compound found in propolis extracts.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the inhibition of biofilm formation and the reduction of preformed or established biofilm by etha- nol extract of propolis samples EEP obtained from Manisa-Turkey was investigated and their chemical com- position was screened.
📊
Key Findings & Data:The antibiofilm effect of the propolis extracts against biofilm forming bacteria Listeria monocytogenes ATCC 7644, Methicillin Sensitive Staphylococcus aureus clinical isolate MSSA M20, S. aureus ATCC 33862, S. aureus ATCC 29213, Enterococcus faecalis ATCC 19433, Pseudomonas fluorescens ATCC 55241, Micrococcus luteus NRRL-B1013 was tested on 96-well polystyrene plates using crystal violet assay.
💡
Conclusion & Impact:According to GC/ MSD analyze, triacontyl acetate was the major compound found in propolis extracts.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Antibiyofilm · Biyofilm indirgeme · Propolis

Abstract

In this study, the inhibition of biofilm formation and the reduction of preformed or established biofilm by etha- nol extract of propolis samples EEP obtained from Manisa-Turkey was investigated and their chemical com- position was screened. The antibiofilm effect of the propolis extracts against biofilm forming bacteria Listeria monocytogenes ATCC 7644, Methicillin Sensitive Staphylococcus aureus clinical isolate MSSA M20, S. aureus ATCC 33862, S. aureus ATCC 29213, Enterococcus faecalis ATCC 19433, Pseudomonas fluorescens ATCC 55241, Micrococcus luteus NRRL-B1013 was tested on 96-well polystyrene plates using crystal violet assay. Also, the antibacterial activity of EEP was evaluated according to Agar Well Diffusion method. Chemical composition of extracts was detected by Gas Chromatography-Mass Spectrometry GC/MSD analyze. The EEP samples exhi- bited good antibiofilm activity against bacteria. The maximum biofilm inhibition activity percentage of MP-1 Manisa-Köprübaşı , MP-2 Manisa-Demirci and MP-3 Manisa-Kula were found as 89.4%, 80.0% and 89.0% for L. monocytogenes ATCC 7644 and 66.0%, 67.0% and 74.0% for MSSA M20, respectively. According to GC/ MSD analyze, triacontyl acetate was the major compound found in propolis extracts.

Özet

B u çalışmada, Manisa’dan elde edilen propolis örneklerinin etanol özütlerinin EEP biyofilm oluşumunun inhibisyonu ve oluşmuş biyofilm tabakasının giderimi üzerine etkileri ile kimyasal kompozisyonları araştırılmıştır. Propolis özütlerinin antibiyofilm etkisi biyofilm oluşturan bakterilere karşı Listeria monocytogenes ATCC 7644, Methicillin Sensitive Staphylococcus aureus clinical isolate MSSA M20, S. aureus ATCC 33862, S. aureus ATCC 29213, Enterococcus faecalis ATCC 19433, Pseudomonas fluorescens ATCC 55241, Micrococcus luteus NRRL-B1013 96 kuyucuklu polisitiren plakalarda kristal viole yöntemi kullanılarak test edilmiştir. Ayrıca, EEP’nin antibakteriyel aktivitesi Agar Kuyu Difüzyon yöntemine göre belirlenmiştir. Özütlerin kimyasal kompozisyonu Gaz Kromatografisi-Kütle Spektrometresi analizi GC/MSD ile tespit edilmiştir. EEP örnekleri bakterilere karşı iyi antibiyofilm aktivite sergilemişlerdir. MP-1 Manisa-Köprübaşı , MP-2 ManisaDemirci ve MP-3 Manisa-Kula ’ün maksimum biyofilm inhibisyon aktivitesi oranları L. monocytogenes ATCC 7644 için sırasıyla %89.4; %80.0 ve %89.0 olarak, MSSA M20 için de sırasıyla %66.0; %67.0 ve %74.0 olarak bulunmuştur. GC/MSD analiz sonucuna göre triakotinil asetat propolis özütlerinde bulunan başlıca bileşiktir

🔖

Cite This Article

DOĞANLI, G. A., ÜLGER, G., DOĞAN, N. M. (2016). Antibiofilm Activity and Chemical Contents of Propolis Samples From Manisa-Turkey. Hacettepe Journal of Biology and Chemistry, Cilt: 44 - Sayı: 4, 505–513. https://ajindex.com/articles/255949

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. C. Solano, M. Echeverz, I. Lasa, Biofilm dispersion and quorum sensing, Current Opinion on Microbiology, 18 (2014) 96-104.
  2. L.A. Keskinen, E.C. Todd, E.T. Ryser, Impact of bacterial stress and biofilm forming ability on transfer of surface-dried Listeria monocytogenes during slicing of delicatessen meats, International Journal of Food Microbiology, 127 (2008) 298-304.
  3. K. Sauer, E. Thatcher, R. Northey, A.A. Gutierrez, Neutral super-oxidised solutions are effective in killing P. aeruginosa biofilms, Biofouling, 25 (2009) 45-54.
  4. H.C. Flemming, J. Wingender, The biofilm matrix, Nature Reviews Microbiology, 9 (2010) 623-633.
  5. S. Esposito, S.M. Purrello, E. Bonnet, A. Novelli, F. Tripodi, R. Pascale, S. Unal, G. Milkovich, Central venous catheter-related biofilm infections: An up- to-date focus on meticillin-resistant Staphylococcus aureus, Journal of Global Antimicrobial Resistance, 1 (2013) 71-78.
  6. J.W. Costerton, P.S. Stewart, E.P. Greenberg, Bacterial biofilms: a common cause of persistent infections, Science, 284 (1999) 1318-1322. 7. S. Fujimura, T. Sato, T. Kikuchi, J. Zaini, K. Gomi, A. Watanabe, Efficacy of clarithromycin plus vancomycin in mice with implant-related infection caused by biofilm forming Staphylococcus aureus, Journal of Orthopaedic Science, 14 (2009) 658-661.
  7. T. Hertiani, R. Edrada-Ebel, S. Ortlepp, R.W. Van Soest, N.J. De Voogd, V. Wray, U. Hentschel, S. Kozytska, W.E. Muller, P. Proksch, From anti-fouling to biofilm inhibition: new cytotoxic secondary metabolites from two Indonesian Agelas sponges, Bioorganic and Medicinal Chemistry, 18 (2010) 1297-1311.
  8. N. Ooi, K. Miller, C. Randall, W. Rhys-Williams, W. Love, I. Chopra, XF-70 and XF-73, novel antibacterial agents active against slow-growing and non-dividing cultures of Staphylococcus aureus including biofilms, Journal of Antimicrobial Chemotherapy, 65 (2010) 72-78.
  9. A. Cuesta, A. Rodriguez, M.A. Esteban, J. Meseguer, In vivo effects of propolis, a honeybee product, on gilthead seabream innate immune responses, Fish and Shellfish Immunology, 18 (2005) 71-80.
  10. I. Jasprica, A. Mornar, Z. Debeljak, A. Smolcic-Bubalo, M. Medic-Saric, L. Mayer et al, In vivo study of propolis supplementation effects on anti-oxidative status and red blood cells, Journal of Ethnopharmacology, 110 (2007) 548-554.
  11. M.C. Marcucci, F. Ferreres, C. Garcia-Viguera, V.S. Bankova, S.L. De Castro, A.P. Dantas, et al, Phenolic compounds from Brazilian propolis with Ethnopharmacology, 74 (2001) 105-112. activities, Journal of