Adsorption of BSA on Metal Loaded Monosize p GMA Microspheres

Objective & Hypothesis:Immobilized metal ion affinity chromatography IMAC is an efficient method for the adsorption of histidine- having proteins so poly glycidyl methacrylate [p GMA ] monosize microspheres with an average diameter of 1.6 μm were produced by dispersion polymerization for adsorption of bovine serum albumin.

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Key Findings & Data:The maximum adsorption capacity of the p GMA -IDA-Cu II microspheres was found to be 278.6 mg/g polymer at pH 5.0.

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Conclusion & Impact:The obtained results showed that the metal chelated p GMA -IDA-Cu II microspheres can be evaluated as an efficicent adsorbent for albumin adsorption.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:İmmobilize metal iyon afinite kromatografi · Albumin · Proteomik

Abstract

Immobilized metal ion affinity chromatography IMAC is an efficient method for the adsorption of histidine- having proteins so poly glycidyl methacrylate [p GMA ] monosize microspheres with an average diameter of 1.6 μm were produced by dispersion polymerization for adsorption of bovine serum albumin. Cu II ions were chelated on the poly glycidyl methacrylate -iminodiacetic acid [p GMA -IDA] microspheres to be used in bovine serum albumin adsorption studies in batch system. Characterizations of the microspheres were carried out by scanning electron microscopy SEM , elemental analysis, Fourier transform infrared FTIR spectroscopy. The maximum adsorption capacity of the p GMA -IDA-Cu II microspheres was found to be 278.6 mg/g polymer at pH 5.0. The elution studies were performed by 1.0 M NaCl solution. The obtained results showed that the metal chelated p GMA -IDA-Cu II microspheres can be evaluated as an efficicent adsorbent for albumin adsorption.

Özet

İ mmobilize metal iyon afinite kromatografi İMAK , histidin taşıyan proteinlerin adsorpsiyonu için etkili bir yöntem olduğundan sığır serum albuminin uzaklaştırılması için dispersiyon polimerizasyon yöntemiyle, 1.6 µm çapında eş boyutlu poli glisidil metakrilat [p GMA ] mikroküreler üretilmiştir. Cu II iyonları, kesikli sistemde sığır serum albuminin adsorpsiyon çalışmalarında kullanılmak üzere p GMA -IDA mikroküreleri ile şelatlanmıştır. Mikrokürelerin karakterizasyonu taramalı elektron mikroskobu SEM , elementel analiz, Fourier dönüşüm kızılötesi FTIR spektroskopisi kullanılarak gerçekleştirilmiştir. p GMA -IDA-Cu II mikrokürelerinin maksimum adsorpsiyon kapasitesi pH 5.0’te 278.6 mg/g polimer bulunmuştur. Elüsyon çalışmaları 1.0 M NaCl çözeltisi ile gerçekleştirilmiştir. Elde edilen sonuçlar p GMA -IDA-Cu II mikrokürelerin albüminin adsorpsiyonu için etkin bir adsorban olarak kabul edilebileceğini göstermiştir

Keywords

İmmobilize metal iyon afinite kromatografi Albumin Proteomik Poli glisidil metakrilat Immobilized metal ion affinity chromatography Proteomics Poly glycidyl methacrylate

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Cite This Article

ALTUNTAŞ, E. B., KARTAL, F., TÜRKMEN, D., DERAZSHAMSHİR, A., DENİZLİ, A. (2015). Adsorption of BSA on Metal Loaded Monosize p GMA Microspheres. Hacettepe Journal of Biology and Chemistry, Cilt: 43 - Sayı: 3, 225–233. https://ajindex.com/articles/255814

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