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Journals / Hacettepe Journal of Biology and Chemistry / 2018 / Cilt: 46 - Sayı: 1

Synthesis and Characterisation of a New Hydrophilic Interaction/Reversed Phase Mixed-Mode Chromatographic Stationary Phase

Hayriye ARAL
Pages
43–52
Publication Date
2018-03-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The stationary phase has also chirality and can be used in enantioseparation of racemic compounds in normal phase chromatography (NPLC).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:A novel multifunctional stationary phase based the amide-silica was synthesised starting from L-isoleucine and 2-aminoacetanilide.
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Key Findings & Data:Hereby, compounds 1, 2, 3 and 4 were synthesised for the first time and 1, 2 and 4 were identified.
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Conclusion & Impact:The stationary phase has also chirality and can be used in enantioseparation of racemic compounds in normal phase chromatography (NPLC).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Mixed-mode stationary phase · Hydrophilic interaction liquid chromatography (HILIC) · Isoleucine

Abstract

A novel multifunctional stationary phase based the amide-silica was synthesised starting from L-isoleucine and 2-aminoacetanilide. Hereby, compounds 1, 2, 3 and 4 were synthesised for the first time and 1, 2 and 4 were identified. The stationary phase was synthesised by a reliable and repeatable method and characterized by elemental analysis, solid state 13C NMR, scanning electron microscope (SEM), and Brunauer, Emmett and Teller (BET). This stationary phase possess four amide groups as polar cites, phenyl ring as aromatic non-polar cite and isopropyl as an aliphatic non-polar cite. Therefore, it can act as both hydrophilic interaction (HILIC) and reversed phase (RPLC) chromatographic stationary phase. The stationary phase has also chirality and can be used in enantioseparation of racemic compounds in normal phase chromatography (NPLC).

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Cite This Article

ARAL, H. (2018). Synthesis and Characterisation of a New Hydrophilic Interaction/Reversed Phase Mixed-Mode Chromatographic Stationary Phase. Hacettepe Journal of Biology and Chemistry, Cilt: 46 - Sayı: 1, 43–52. https://ajindex.com/articles/255741

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References (13)

  1. L. Zhang, Q. Dai, X. Qiao, C. Yu, X. Qin, H. Yan, Mixed-mode chromatographic stationary phases: Recent advancement and its aplications for highperformance liquid chromatography, Trends Anal. Chem., 82 (2016) 143-163.
  2. P. Janas, S. Bocian, P. Jandera, T. Kowalkowski, B. Buszewski, Separation of flavonoids on different phenyl-bonded stationary phases-the influence of polar groups in stationary phase structure, J. Chromatogr. A, 1429 (2016) 198-206.
  3. Y. Liu, Z. Guo, Y. Jin, X. Xue, Q. Xu, F. Zhang, et al., Click oligo(ethylene glycol)”: an excellent orthogonal stationary phase to C18 for twodimensional reversedphase/ reversed-phase liquid chromatography, J. Chromatogr. A, 1206 (2008) 153- 159. P. Jandera, Z. Kucˇerova, J. Urban, Retention times and bandwidths in reversedphase gradient liquid chromatography of peptides and proteins, J. Chromatogr. A, 1218 (2011) 8874–8889.
  4. X. Guo, X. Zhang, Z. Guo, Y. Liu, A. Shen, G. Jin, et al., Hydrophilic interaction chromatography for selective separation of isomeric saponins, J. Chromatogr. A, 1325 (2014) 121-128.
  5. P. Jiang, D.Wu, C.A. Lucy, Determination of void volume in normal phase liquid chromatography, J. Chromatogr. A, 1324 (2014) 63-70.
  6. A.J. Alpert, M. Shukla, A.K. Shukla, L.R. Zieske, S.W. Yuen, M.A.J. Ferguson, A. Mehlert, M. Pauly, R. Orlando, Hydrophilic-interaction chromatography of complex carbohydrates, J. Chromatogr. A, 676 (1994) 191-202
  7. A.J. Alpert, Advances in Chromatography, 44 (2006) 317-329.
  8. M. R. Gama, R. G. C. Silva, C. H. Collins, C. B. G. Bottoli, Hydrophylic interaction chromatography, Trends in Anal. Chem. 37 (2012) 48-60
  9. M.A. Strege, S. Stevensen, S. M.Lawrence, Mixed-mode anion−cation exchange/hydrophilic interaction liquid chromatography−electrospray mass spectrometry as an alternative to reversed phase for small molecule drug discovery, Anal. Chem., 72 (2000) 4629-4633
  10. M.A. Strege, Hydrophilic interaction chromatography− electrospray mass spectrometry analysis of polar compounds for natural product drug discovery, Anal. Chem., 70 (1998) 2439-2445
  11. B.Y. Zhu, C.T. Colin, R.S. Hodges, Hydrophilicinteraction chromatography of peptides on hydrophilic and strong cation-exchange columns, J. Chromatogr., 548 (1991) 13-24.
  12. T. Saga, Y. Inoue, K. Yamaguchi, Determination of carbohydrates by hydrophilic interaction chromatography with pulsed amperometric detection using postcolumn pH adjustment, J. Chromatogr. A, 625 (1992) 151-155.
  13. Q. W. Yu, B. Lin, Y. Q. Feng, F. P. Zou, Application of humic acid bonded